A nanocompartment system contributes to defense against oxidative stress in Mycobacterium tuberculosis

Encapsulin nanocompartments are an emerging class of prokaryotic protein-based organelle consisting of an encapsulin protein shell that encloses a protein cargo. Genes encoding nanocompartments are widespread in bacteria and archaea, and recent works have characterized the biochemical function of se...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Katie A Lien, Kayla Dinshaw, Robert J Nichols, Caleb Cassidy-Amstutz, Matthew Knight, Rahul Singh, Lindsay D Eltis, David F Savage, Sarah A Stanley
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	Mycobacterium tuberculosis encapsulin oxidative defense Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/b3fff152f7ee4ad4a13e56e9e94db622
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b3fff152f7ee4ad4a13e56e9e94db622
record_format	dspace
spelling	oai:doaj.org-article:b3fff152f7ee4ad4a13e56e9e94db6222021-12-01T15:40:49ZA nanocompartment system contributes to defense against oxidative stress in Mycobacterium tuberculosis10.7554/eLife.743582050-084Xe74358https://doaj.org/article/b3fff152f7ee4ad4a13e56e9e94db6222021-11-01T00:00:00Zhttps://elifesciences.org/articles/74358https://doaj.org/toc/2050-084XEncapsulin nanocompartments are an emerging class of prokaryotic protein-based organelle consisting of an encapsulin protein shell that encloses a protein cargo. Genes encoding nanocompartments are widespread in bacteria and archaea, and recent works have characterized the biochemical function of several cargo enzymes. However, the importance of these organelles to host physiology is poorly understood. Here, we report that the human pathogen Mycobacterium tuberculosis (Mtb) produces a nanocompartment that contains the dye-decolorizing peroxidase DyP. We show that this nanocompartment is important for the ability of Mtb to resist oxidative stress in low pH environments, including during infection of host cells and upon treatment with a clinically relevant antibiotic. Our findings are the first to implicate a nanocompartment in bacterial pathogenesis and reveal a new mechanism that Mtb uses to combat oxidative stress.Katie A LienKayla DinshawRobert J NicholsCaleb Cassidy-AmstutzMatthew KnightRahul SinghLindsay D EltisDavid F SavageSarah A StanleyeLife Sciences Publications LtdarticleMycobacterium tuberculosisencapsulinoxidative defenseMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Mycobacterium tuberculosis encapsulin oxidative defense Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	Mycobacterium tuberculosis encapsulin oxidative defense Medicine R Science Q Biology (General) QH301-705.5 Katie A Lien Kayla Dinshaw Robert J Nichols Caleb Cassidy-Amstutz Matthew Knight Rahul Singh Lindsay D Eltis David F Savage Sarah A Stanley A nanocompartment system contributes to defense against oxidative stress in Mycobacterium tuberculosis
description	Encapsulin nanocompartments are an emerging class of prokaryotic protein-based organelle consisting of an encapsulin protein shell that encloses a protein cargo. Genes encoding nanocompartments are widespread in bacteria and archaea, and recent works have characterized the biochemical function of several cargo enzymes. However, the importance of these organelles to host physiology is poorly understood. Here, we report that the human pathogen Mycobacterium tuberculosis (Mtb) produces a nanocompartment that contains the dye-decolorizing peroxidase DyP. We show that this nanocompartment is important for the ability of Mtb to resist oxidative stress in low pH environments, including during infection of host cells and upon treatment with a clinically relevant antibiotic. Our findings are the first to implicate a nanocompartment in bacterial pathogenesis and reveal a new mechanism that Mtb uses to combat oxidative stress.
format	article
author	Katie A Lien Kayla Dinshaw Robert J Nichols Caleb Cassidy-Amstutz Matthew Knight Rahul Singh Lindsay D Eltis David F Savage Sarah A Stanley
author_facet	Katie A Lien Kayla Dinshaw Robert J Nichols Caleb Cassidy-Amstutz Matthew Knight Rahul Singh Lindsay D Eltis David F Savage Sarah A Stanley
author_sort	Katie A Lien
title	A nanocompartment system contributes to defense against oxidative stress in Mycobacterium tuberculosis
title_short	A nanocompartment system contributes to defense against oxidative stress in Mycobacterium tuberculosis
title_full	A nanocompartment system contributes to defense against oxidative stress in Mycobacterium tuberculosis
title_fullStr	A nanocompartment system contributes to defense against oxidative stress in Mycobacterium tuberculosis
title_full_unstemmed	A nanocompartment system contributes to defense against oxidative stress in Mycobacterium tuberculosis
title_sort	nanocompartment system contributes to defense against oxidative stress in mycobacterium tuberculosis
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/b3fff152f7ee4ad4a13e56e9e94db622
work_keys_str_mv	AT katiealien ananocompartmentsystemcontributestodefenseagainstoxidativestressinmycobacteriumtuberculosis AT kayladinshaw ananocompartmentsystemcontributestodefenseagainstoxidativestressinmycobacteriumtuberculosis AT robertjnichols ananocompartmentsystemcontributestodefenseagainstoxidativestressinmycobacteriumtuberculosis AT calebcassidyamstutz ananocompartmentsystemcontributestodefenseagainstoxidativestressinmycobacteriumtuberculosis AT matthewknight ananocompartmentsystemcontributestodefenseagainstoxidativestressinmycobacteriumtuberculosis AT rahulsingh ananocompartmentsystemcontributestodefenseagainstoxidativestressinmycobacteriumtuberculosis AT lindsaydeltis ananocompartmentsystemcontributestodefenseagainstoxidativestressinmycobacteriumtuberculosis AT davidfsavage ananocompartmentsystemcontributestodefenseagainstoxidativestressinmycobacteriumtuberculosis AT sarahastanley ananocompartmentsystemcontributestodefenseagainstoxidativestressinmycobacteriumtuberculosis AT katiealien nanocompartmentsystemcontributestodefenseagainstoxidativestressinmycobacteriumtuberculosis AT kayladinshaw nanocompartmentsystemcontributestodefenseagainstoxidativestressinmycobacteriumtuberculosis AT robertjnichols nanocompartmentsystemcontributestodefenseagainstoxidativestressinmycobacteriumtuberculosis AT calebcassidyamstutz nanocompartmentsystemcontributestodefenseagainstoxidativestressinmycobacteriumtuberculosis AT matthewknight nanocompartmentsystemcontributestodefenseagainstoxidativestressinmycobacteriumtuberculosis AT rahulsingh nanocompartmentsystemcontributestodefenseagainstoxidativestressinmycobacteriumtuberculosis AT lindsaydeltis nanocompartmentsystemcontributestodefenseagainstoxidativestressinmycobacteriumtuberculosis AT davidfsavage nanocompartmentsystemcontributestodefenseagainstoxidativestressinmycobacteriumtuberculosis AT sarahastanley nanocompartmentsystemcontributestodefenseagainstoxidativestressinmycobacteriumtuberculosis
_version_	1718404836845682688

A nanocompartment system contributes to defense against oxidative stress in Mycobacterium tuberculosis

Ejemplares similares