Regulation of indoleamine 2,3-dioxygenase in primary human saphenous vein endothelial cells

Petros XE Mouratidis,* Andrew JT George*  Department of Immunology, Imperial College London, London, UK *These authors contributed equally to this work Background: Indoleamine 2,3-dioxygenase (IDO) is an enzyme associated with the regulation of immune responses. Cytokines such as IFN&g...

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Autores principales:	Mouratidis PX, George AJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/b402d1a8a70142638dbc9815ed38f123
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spelling	oai:doaj.org-article:b402d1a8a70142638dbc9815ed38f1232021-12-02T02:11:06ZRegulation of indoleamine 2,3-dioxygenase in primary human saphenous vein endothelial cells1178-7031https://doaj.org/article/b402d1a8a70142638dbc9815ed38f1232015-05-01T00:00:00Zhttp://www.dovepress.com/regulation-of-indoleamine-23-dioxygenase-in-primary-human-saphenous-ve-peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Petros XE Mouratidis,* Andrew JT George*  Department of Immunology, Imperial College London, London, UK *These authors contributed equally to this work Background: Indoleamine 2,3-dioxygenase (IDO) is an enzyme associated with the regulation of immune responses. Cytokines such as IFNγ induce its expression in endothelial cells originating from immune-privileged sites. In this study, we investigate regulators of IDO in primary endothelial cells from a non-immune-privileged site and determine whether IDO expression affects immune cell behavior.Methods: IDO expression was determined using real-time quantitative polymerase chain reaction and immunoblotting. IDO activity was estimated using an IDO enzyme assay. Primary cells were transfected using microporation, and T-cell migration was determined using a cell transmigration assay.Results: IDO is expressed in human saphenous vein endothelial cells after stimulation with IFNγ but not after treatment with TNFα, IL-1β, IL-2, IL-4, IL-6, or IL-10. VEGFβ and heparin negatively regulate IFNγ-driven increases in IDO. Overexpression of IDO in endothelial cells does not affect transmigration of T-cells.Conclusion: IDO is expressed in human saphenous vein endothelial cells after stimulation with IFNγ. Heparin and angiogenesis stimulators such as VEGFβ negatively regulate its expression. Keywords: IFNγ, VEGFβ, endothelium, inflammation, HSVECMouratidis PXGeorge AJDove Medical PressarticlePathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol 2015, Iss default, Pp 97-106 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Mouratidis PX George AJ Regulation of indoleamine 2,3-dioxygenase in primary human saphenous vein endothelial cells
description	Petros XE Mouratidis,* Andrew JT George*  Department of Immunology, Imperial College London, London, UK *These authors contributed equally to this work Background: Indoleamine 2,3-dioxygenase (IDO) is an enzyme associated with the regulation of immune responses. Cytokines such as IFNγ induce its expression in endothelial cells originating from immune-privileged sites. In this study, we investigate regulators of IDO in primary endothelial cells from a non-immune-privileged site and determine whether IDO expression affects immune cell behavior.Methods: IDO expression was determined using real-time quantitative polymerase chain reaction and immunoblotting. IDO activity was estimated using an IDO enzyme assay. Primary cells were transfected using microporation, and T-cell migration was determined using a cell transmigration assay.Results: IDO is expressed in human saphenous vein endothelial cells after stimulation with IFNγ but not after treatment with TNFα, IL-1β, IL-2, IL-4, IL-6, or IL-10. VEGFβ and heparin negatively regulate IFNγ-driven increases in IDO. Overexpression of IDO in endothelial cells does not affect transmigration of T-cells.Conclusion: IDO is expressed in human saphenous vein endothelial cells after stimulation with IFNγ. Heparin and angiogenesis stimulators such as VEGFβ negatively regulate its expression. Keywords: IFNγ, VEGFβ, endothelium, inflammation, HSVEC
format	article
author	Mouratidis PX George AJ
author_facet	Mouratidis PX George AJ
author_sort	Mouratidis PX
title	Regulation of indoleamine 2,3-dioxygenase in primary human saphenous vein endothelial cells
title_short	Regulation of indoleamine 2,3-dioxygenase in primary human saphenous vein endothelial cells
title_full	Regulation of indoleamine 2,3-dioxygenase in primary human saphenous vein endothelial cells
title_fullStr	Regulation of indoleamine 2,3-dioxygenase in primary human saphenous vein endothelial cells
title_full_unstemmed	Regulation of indoleamine 2,3-dioxygenase in primary human saphenous vein endothelial cells
title_sort	regulation of indoleamine 2,3-dioxygenase in primary human saphenous vein endothelial cells
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/b402d1a8a70142638dbc9815ed38f123
work_keys_str_mv	AT mouratidispx regulationofindoleamine23dioxygenaseinprimaryhumansaphenousveinendothelialcells AT georgeaj regulationofindoleamine23dioxygenaseinprimaryhumansaphenousveinendothelialcells
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Regulation of indoleamine 2,3-dioxygenase in primary human saphenous vein endothelial cells

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