Identification of spectral modifications occurring during reprogramming of somatic cells.
Recent technological advances in cell reprogramming by generation of induced pluripotent stem cells (iPSC) offer major perspectives in disease modelling and future hopes for providing novel stem cells sources in regenerative medicine. However, research on iPSC still requires refining the criteria of...
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2012
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oai:doaj.org-article:b4035ce8299448e7aa2e8624dfe9aca92021-11-18T07:22:23ZIdentification of spectral modifications occurring during reprogramming of somatic cells.1932-620310.1371/journal.pone.0030743https://doaj.org/article/b4035ce8299448e7aa2e8624dfe9aca92012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22514597/?tool=EBIhttps://doaj.org/toc/1932-6203Recent technological advances in cell reprogramming by generation of induced pluripotent stem cells (iPSC) offer major perspectives in disease modelling and future hopes for providing novel stem cells sources in regenerative medicine. However, research on iPSC still requires refining the criteria of the pluripotency stage of these cells and exploration of their equivalent functionality to human embryonic stem cells (ESC). We report here on the use of infrared microspectroscopy to follow the spectral modification of somatic cells during the reprogramming process. We show that induced pluripotent stem cells (iPSC) adopt a chemical composition leading to a spectral signature indistinguishable from that of embryonic stem cells (ESC) and entirely different from that of the original somatic cells. Similarly, this technique allows a distinction to be made between partially and fully reprogrammed cells. We conclude that infrared microspectroscopy signature is a novel methodology to evaluate induced pluripotency and can be added to the tests currently used for this purpose.Christophe SandtOlivier FéraudNoufissa OudrhiriMarie Laure BonnetMarie Claude MeunierYannick ValogneAngelina BertrandMartine RaphaëlFrank GriscelliAli G TurhanPaul DumasAnnelise Bennaceur-GriscelliPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e30743 (2012) |
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EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Christophe Sandt Olivier Féraud Noufissa Oudrhiri Marie Laure Bonnet Marie Claude Meunier Yannick Valogne Angelina Bertrand Martine Raphaël Frank Griscelli Ali G Turhan Paul Dumas Annelise Bennaceur-Griscelli Identification of spectral modifications occurring during reprogramming of somatic cells. |
| description |
Recent technological advances in cell reprogramming by generation of induced pluripotent stem cells (iPSC) offer major perspectives in disease modelling and future hopes for providing novel stem cells sources in regenerative medicine. However, research on iPSC still requires refining the criteria of the pluripotency stage of these cells and exploration of their equivalent functionality to human embryonic stem cells (ESC). We report here on the use of infrared microspectroscopy to follow the spectral modification of somatic cells during the reprogramming process. We show that induced pluripotent stem cells (iPSC) adopt a chemical composition leading to a spectral signature indistinguishable from that of embryonic stem cells (ESC) and entirely different from that of the original somatic cells. Similarly, this technique allows a distinction to be made between partially and fully reprogrammed cells. We conclude that infrared microspectroscopy signature is a novel methodology to evaluate induced pluripotency and can be added to the tests currently used for this purpose. |
| format |
article |
| author |
Christophe Sandt Olivier Féraud Noufissa Oudrhiri Marie Laure Bonnet Marie Claude Meunier Yannick Valogne Angelina Bertrand Martine Raphaël Frank Griscelli Ali G Turhan Paul Dumas Annelise Bennaceur-Griscelli |
| author_facet |
Christophe Sandt Olivier Féraud Noufissa Oudrhiri Marie Laure Bonnet Marie Claude Meunier Yannick Valogne Angelina Bertrand Martine Raphaël Frank Griscelli Ali G Turhan Paul Dumas Annelise Bennaceur-Griscelli |
| author_sort |
Christophe Sandt |
| title |
Identification of spectral modifications occurring during reprogramming of somatic cells. |
| title_short |
Identification of spectral modifications occurring during reprogramming of somatic cells. |
| title_full |
Identification of spectral modifications occurring during reprogramming of somatic cells. |
| title_fullStr |
Identification of spectral modifications occurring during reprogramming of somatic cells. |
| title_full_unstemmed |
Identification of spectral modifications occurring during reprogramming of somatic cells. |
| title_sort |
identification of spectral modifications occurring during reprogramming of somatic cells. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/b4035ce8299448e7aa2e8624dfe9aca9 |
| work_keys_str_mv |
AT christophesandt identificationofspectralmodificationsoccurringduringreprogrammingofsomaticcells AT olivierferaud identificationofspectralmodificationsoccurringduringreprogrammingofsomaticcells AT noufissaoudrhiri identificationofspectralmodificationsoccurringduringreprogrammingofsomaticcells AT marielaurebonnet identificationofspectralmodificationsoccurringduringreprogrammingofsomaticcells AT marieclaudemeunier identificationofspectralmodificationsoccurringduringreprogrammingofsomaticcells AT yannickvalogne identificationofspectralmodificationsoccurringduringreprogrammingofsomaticcells AT angelinabertrand identificationofspectralmodificationsoccurringduringreprogrammingofsomaticcells AT martineraphael identificationofspectralmodificationsoccurringduringreprogrammingofsomaticcells AT frankgriscelli identificationofspectralmodificationsoccurringduringreprogrammingofsomaticcells AT aligturhan identificationofspectralmodificationsoccurringduringreprogrammingofsomaticcells AT pauldumas identificationofspectralmodificationsoccurringduringreprogrammingofsomaticcells AT annelisebennaceurgriscelli identificationofspectralmodificationsoccurringduringreprogrammingofsomaticcells |
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1718423557588910080 |