Netosis in patients with systemic lupus erythematosus

The article presents the data on assessment of functional features of neutrophils in 34 patients with systemic lupus erythematosus (SLE). Development of neutrophil extracellular traps (NETs) was evaluated in cell cultures incubated in vitro for 30 and 150 minutes (basal levels, NETBAS30 and NETBAS15...

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Autores principales:	V. V. Zhelezko, I. A. Novikova
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2020
Materias:	systemic lupus erythematosus neutrophilic extracellular traps Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/b408af89307b46b7947847b333dc7f0d
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spelling	oai:doaj.org-article:b408af89307b46b7947847b333dc7f0d2021-11-18T08:03:50ZNetosis in patients with systemic lupus erythematosus1563-06252313-741X10.15789/1563-0625-NIP-1978https://doaj.org/article/b408af89307b46b7947847b333dc7f0d2020-12-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1978https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XThe article presents the data on assessment of functional features of neutrophils in 34 patients with systemic lupus erythematosus (SLE). Development of neutrophil extracellular traps (NETs) was evaluated in cell cultures incubated in vitro for 30 and 150 minutes (basal levels, NETBAS30 and NETBAS150, respectively), and in the presence of heat-inactivated S. аureus (strain ATCC 25923, 108 CFU/ml) (stimulated levels, NETST30 and NETST150, respectively). NET looks like thin free-lying extracellular fibrillar structures, 2-3 times exceeding the size of unchanged granulocyte. The result was expressed as percentage and relative amount of extracellular traps per 100 counted leukocytes. Phagocytic activity of neutrophils was evaluated as phagocytosis of S. аureus by counting the percentage of neutrophils that engulfed phagocytic index of microbial particles (PI); the average number of phagocytosed objects per neutrophil phagocytic number (PC). ROS-producing activity was determined in the reduction of Nitroblue Tetrazolium tested in spontaneous and stimulated S. аureus variants (NBTBAS and NBTST, respectively). The result was expressed as the percentage of formazan-positive cells per 100 white blood cells. Nitroxide-producing properties were determined using the Crow (1999) method in spontaneous and stimulated samples for the accumulation of the nitrated amino acid tyrosine (3-nitrothyrosine, 3-NTBAS, and 3-NTST, respectively). We revealed a decrease in ROS production, phagocytosis and NO-forming activity of neutrophils associated with increased netosis. Activation of the netosis was observed in cell cultures without stimulation, indicating the in vivo formation of networks in SLE. The NET increase is most pronounced in the patients with lupus nephritis (p < 0.05), and in remission of the disease (p < 0.05). We have revealed a correlation of NET formation parameters with duration and degree of SLE activity (rs = -0.6; p = 0.001, and rs = 0.39; p = 0.02, respectively); autoantibody titers (anti-dsDNA and ANA) (rs = 0.67; р = 0.047 and rs = 0.59; р = 0.034, respectively); prothrombin complex activity (rs = 0.6; p = 0.036), as well and urea and creatinine levels (rs = 0.47; p = 0.037 and rs = 0.39; p = 0.048, respectively). The parameters of NETs can be considered a promising biomarker for verifying the diagnosis of SLE, evaluation of clinical activity, disease severity, and predicting the development of complications.V. V. ZhelezkoI. A. NovikovaSPb RAACIarticlesystemic lupus erythematosusneutrophilic extracellular trapsImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 22, Iss 5, Pp 987-992 (2020)
institution	DOAJ
collection	DOAJ
language	RU
topic	systemic lupus erythematosus neutrophilic extracellular traps Immunologic diseases. Allergy RC581-607
spellingShingle	systemic lupus erythematosus neutrophilic extracellular traps Immunologic diseases. Allergy RC581-607 V. V. Zhelezko I. A. Novikova Netosis in patients with systemic lupus erythematosus
description	The article presents the data on assessment of functional features of neutrophils in 34 patients with systemic lupus erythematosus (SLE). Development of neutrophil extracellular traps (NETs) was evaluated in cell cultures incubated in vitro for 30 and 150 minutes (basal levels, NETBAS30 and NETBAS150, respectively), and in the presence of heat-inactivated S. аureus (strain ATCC 25923, 108 CFU/ml) (stimulated levels, NETST30 and NETST150, respectively). NET looks like thin free-lying extracellular fibrillar structures, 2-3 times exceeding the size of unchanged granulocyte. The result was expressed as percentage and relative amount of extracellular traps per 100 counted leukocytes. Phagocytic activity of neutrophils was evaluated as phagocytosis of S. аureus by counting the percentage of neutrophils that engulfed phagocytic index of microbial particles (PI); the average number of phagocytosed objects per neutrophil phagocytic number (PC). ROS-producing activity was determined in the reduction of Nitroblue Tetrazolium tested in spontaneous and stimulated S. аureus variants (NBTBAS and NBTST, respectively). The result was expressed as the percentage of formazan-positive cells per 100 white blood cells. Nitroxide-producing properties were determined using the Crow (1999) method in spontaneous and stimulated samples for the accumulation of the nitrated amino acid tyrosine (3-nitrothyrosine, 3-NTBAS, and 3-NTST, respectively). We revealed a decrease in ROS production, phagocytosis and NO-forming activity of neutrophils associated with increased netosis. Activation of the netosis was observed in cell cultures without stimulation, indicating the in vivo formation of networks in SLE. The NET increase is most pronounced in the patients with lupus nephritis (p < 0.05), and in remission of the disease (p < 0.05). We have revealed a correlation of NET formation parameters with duration and degree of SLE activity (rs = -0.6; p = 0.001, and rs = 0.39; p = 0.02, respectively); autoantibody titers (anti-dsDNA and ANA) (rs = 0.67; р = 0.047 and rs = 0.59; р = 0.034, respectively); prothrombin complex activity (rs = 0.6; p = 0.036), as well and urea and creatinine levels (rs = 0.47; p = 0.037 and rs = 0.39; p = 0.048, respectively). The parameters of NETs can be considered a promising biomarker for verifying the diagnosis of SLE, evaluation of clinical activity, disease severity, and predicting the development of complications.
format	article
author	V. V. Zhelezko I. A. Novikova
author_facet	V. V. Zhelezko I. A. Novikova
author_sort	V. V. Zhelezko
title	Netosis in patients with systemic lupus erythematosus
title_short	Netosis in patients with systemic lupus erythematosus
title_full	Netosis in patients with systemic lupus erythematosus
title_fullStr	Netosis in patients with systemic lupus erythematosus
title_full_unstemmed	Netosis in patients with systemic lupus erythematosus
title_sort	netosis in patients with systemic lupus erythematosus
publisher	SPb RAACI
publishDate	2020
url	https://doaj.org/article/b408af89307b46b7947847b333dc7f0d
work_keys_str_mv	AT vvzhelezko netosisinpatientswithsystemiclupuserythematosus AT ianovikova netosisinpatientswithsystemiclupuserythematosus
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Netosis in patients with systemic lupus erythematosus

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