Anti-tumor effect of polysaccharide from Pleurotus ostreatus on H22 mouse Hepatoma ascites in-vivo and hepatocellular carcinoma in-vitro model

Abstract Hepatocellular carcinoma is one of the leading causes of cancer-associated death across the globe. Malignant ascites are the major clinical attributes in cancer patients. Despite the advancements in HCC treatments such as chemotherapy, radiotherapy, surgery, and hormonal therapy, researcher...

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Autores principales:	Kavish Hasnain Khinsar, Sattar Abdul, Akbar Hussain, Riaz Ud Din, Liu Lei, Jing Cao, Majid Abbasi, Ata Ur Rehman, Nabeel Farooqui, Xin Yi, Huang Min, Liang Wang, Zhong Mintao
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Pleurotus ostreatus Hepatocarcinoma Malignant ascites Immunohistochemistry Polysaccharides Biotechnology TP248.13-248.65 Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/b412292fcffb49ef9e37d3e88fc4ffc6
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spelling	oai:doaj.org-article:b412292fcffb49ef9e37d3e88fc4ffc62021-12-05T12:08:33ZAnti-tumor effect of polysaccharide from Pleurotus ostreatus on H22 mouse Hepatoma ascites in-vivo and hepatocellular carcinoma in-vitro model10.1186/s13568-021-01314-52191-0855https://doaj.org/article/b412292fcffb49ef9e37d3e88fc4ffc62021-12-01T00:00:00Zhttps://doi.org/10.1186/s13568-021-01314-5https://doaj.org/toc/2191-0855Abstract Hepatocellular carcinoma is one of the leading causes of cancer-associated death across the globe. Malignant ascites are the major clinical attributes in cancer patients. Despite the advancements in HCC treatments such as chemotherapy, radiotherapy, surgery, and hormonal therapy, researchers are pursuing novel natural edible compounds for the treatment of cancer to eliminate dreadful side effects. Pleurotus ostreatus is one of the most edible cuisines in Asia as well as all over the world. It has been a source of nutritious diet since it was classified as an edible mushroom with no or negligible side effects. The present study focused on the natural anti-cancerous and anti-ascites capabilities of polysaccharides extracted from Pleurotus ostreatus in-vivo as well as in-vitro. Administration of polysaccharide Pleurotus ostreatus showed a significant decrease in tumor cell metastasis while the increase in the survival period among mice models of H22 malignant ascites. Downregulation of regenerative genes Foxp3 and Stat3 and secretion of immunological factors such as IL-2, TNF α, and INF γ were observed after treating with the partially pure extracted polysaccharide. Twining with the hypothesis of tumor suppression in-vivo model polysaccharide showed a decrease in invasion and migration abilities and henceforth responsible for the gene regulation such Cytochrome C which supposedly induced the chain of gene regulation process resulting in apoptosis in HCC cell lines observed in-vitro experiments. Collective research findings manifested that polysaccharide extracted from Pleurotus ostreatus bears anti-proliferative activity and thus influence tumor suppression in-vivo and in-vitro against hepatocellular carcinoma and can be used for therapeutic purposes as a potential anti-cancerous source in the future.Kavish Hasnain KhinsarSattar AbdulAkbar HussainRiaz Ud DinLiu LeiJing CaoMajid AbbasiAta Ur RehmanNabeel FarooquiXin YiHuang MinLiang WangZhong MintaoSpringerOpenarticlePleurotus ostreatusHepatocarcinomaMalignant ascitesImmunohistochemistryPolysaccharidesBiotechnologyTP248.13-248.65MicrobiologyQR1-502ENAMB Express, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pleurotus ostreatus Hepatocarcinoma Malignant ascites Immunohistochemistry Polysaccharides Biotechnology TP248.13-248.65 Microbiology QR1-502
spellingShingle	Pleurotus ostreatus Hepatocarcinoma Malignant ascites Immunohistochemistry Polysaccharides Biotechnology TP248.13-248.65 Microbiology QR1-502 Kavish Hasnain Khinsar Sattar Abdul Akbar Hussain Riaz Ud Din Liu Lei Jing Cao Majid Abbasi Ata Ur Rehman Nabeel Farooqui Xin Yi Huang Min Liang Wang Zhong Mintao Anti-tumor effect of polysaccharide from Pleurotus ostreatus on H22 mouse Hepatoma ascites in-vivo and hepatocellular carcinoma in-vitro model
description	Abstract Hepatocellular carcinoma is one of the leading causes of cancer-associated death across the globe. Malignant ascites are the major clinical attributes in cancer patients. Despite the advancements in HCC treatments such as chemotherapy, radiotherapy, surgery, and hormonal therapy, researchers are pursuing novel natural edible compounds for the treatment of cancer to eliminate dreadful side effects. Pleurotus ostreatus is one of the most edible cuisines in Asia as well as all over the world. It has been a source of nutritious diet since it was classified as an edible mushroom with no or negligible side effects. The present study focused on the natural anti-cancerous and anti-ascites capabilities of polysaccharides extracted from Pleurotus ostreatus in-vivo as well as in-vitro. Administration of polysaccharide Pleurotus ostreatus showed a significant decrease in tumor cell metastasis while the increase in the survival period among mice models of H22 malignant ascites. Downregulation of regenerative genes Foxp3 and Stat3 and secretion of immunological factors such as IL-2, TNF α, and INF γ were observed after treating with the partially pure extracted polysaccharide. Twining with the hypothesis of tumor suppression in-vivo model polysaccharide showed a decrease in invasion and migration abilities and henceforth responsible for the gene regulation such Cytochrome C which supposedly induced the chain of gene regulation process resulting in apoptosis in HCC cell lines observed in-vitro experiments. Collective research findings manifested that polysaccharide extracted from Pleurotus ostreatus bears anti-proliferative activity and thus influence tumor suppression in-vivo and in-vitro against hepatocellular carcinoma and can be used for therapeutic purposes as a potential anti-cancerous source in the future.
format	article
author	Kavish Hasnain Khinsar Sattar Abdul Akbar Hussain Riaz Ud Din Liu Lei Jing Cao Majid Abbasi Ata Ur Rehman Nabeel Farooqui Xin Yi Huang Min Liang Wang Zhong Mintao
author_facet	Kavish Hasnain Khinsar Sattar Abdul Akbar Hussain Riaz Ud Din Liu Lei Jing Cao Majid Abbasi Ata Ur Rehman Nabeel Farooqui Xin Yi Huang Min Liang Wang Zhong Mintao
author_sort	Kavish Hasnain Khinsar
title	Anti-tumor effect of polysaccharide from Pleurotus ostreatus on H22 mouse Hepatoma ascites in-vivo and hepatocellular carcinoma in-vitro model
title_short	Anti-tumor effect of polysaccharide from Pleurotus ostreatus on H22 mouse Hepatoma ascites in-vivo and hepatocellular carcinoma in-vitro model
title_full	Anti-tumor effect of polysaccharide from Pleurotus ostreatus on H22 mouse Hepatoma ascites in-vivo and hepatocellular carcinoma in-vitro model
title_fullStr	Anti-tumor effect of polysaccharide from Pleurotus ostreatus on H22 mouse Hepatoma ascites in-vivo and hepatocellular carcinoma in-vitro model
title_full_unstemmed	Anti-tumor effect of polysaccharide from Pleurotus ostreatus on H22 mouse Hepatoma ascites in-vivo and hepatocellular carcinoma in-vitro model
title_sort	anti-tumor effect of polysaccharide from pleurotus ostreatus on h22 mouse hepatoma ascites in-vivo and hepatocellular carcinoma in-vitro model
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/b412292fcffb49ef9e37d3e88fc4ffc6
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Anti-tumor effect of polysaccharide from Pleurotus ostreatus on H22 mouse Hepatoma ascites in-vivo and hepatocellular carcinoma in-vitro model

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