Visualizing changes in brain-derived neurotrophic factor (BDNF) expression using bioluminescence imaging in living mice

Abstract Brain-derived neurotrophic factor (BDNF) plays a fundamental role in expressing various neural functions including memory consolidation. Alterations of BDNF levels in the brain are associated with neurodegenerative and neuropsychiatric disorders. Therefore, it is important to understand how...

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Autores principales:	Mamoru Fukuchi, Hironori Izumi, Hisashi Mori, Masahiro Kiyama, Satoshi Otsuka, Shojiro Maki, Yosuke Maehata, Akiko Tabuchi, Masaaki Tsuda
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b4141928f2b141788bb1cc12a0d39c29
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spelling	oai:doaj.org-article:b4141928f2b141788bb1cc12a0d39c292021-12-02T15:05:49ZVisualizing changes in brain-derived neurotrophic factor (BDNF) expression using bioluminescence imaging in living mice10.1038/s41598-017-05297-x2045-2322https://doaj.org/article/b4141928f2b141788bb1cc12a0d39c292017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05297-xhttps://doaj.org/toc/2045-2322Abstract Brain-derived neurotrophic factor (BDNF) plays a fundamental role in expressing various neural functions including memory consolidation. Alterations of BDNF levels in the brain are associated with neurodegenerative and neuropsychiatric disorders. Therefore, it is important to understand how levels of BDNF are controlled. Recently we generated a novel transgenic mouse strain, termed the Bdnf-Luciferase transgenic (Bdnf-Luc Tg) mouse, to monitor changes in Bdnf expression. In the present study, we detected the bioluminescence signal from living Bdnf-Luc Tg mice after intraperitoneal administration of d-luciferin. Despite high levels of Bdnf expression in the brain, it was difficult to detect a signal from the brain region, probably because of its poorly penetrable (short-wavelength) bioluminescence. However, we could detect the changes in the bioluminescence signal in the brain region using a luciferin analogue generating a near-infrared wavelength of bioluminescence. We also found a strong correlation between increases in body weight and bioluminescence signal in the abdominal region of Tg mice fed a high-fat diet. These results show that changes in Bdnf expression can be visualized using living mice, and that the Tg mouse could be a powerful tool for clarification of the role of Bdnf expression in pathophysiological and physiological conditions.Mamoru FukuchiHironori IzumiHisashi MoriMasahiro KiyamaSatoshi OtsukaShojiro MakiYosuke MaehataAkiko TabuchiMasaaki TsudaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mamoru Fukuchi Hironori Izumi Hisashi Mori Masahiro Kiyama Satoshi Otsuka Shojiro Maki Yosuke Maehata Akiko Tabuchi Masaaki Tsuda Visualizing changes in brain-derived neurotrophic factor (BDNF) expression using bioluminescence imaging in living mice
description	Abstract Brain-derived neurotrophic factor (BDNF) plays a fundamental role in expressing various neural functions including memory consolidation. Alterations of BDNF levels in the brain are associated with neurodegenerative and neuropsychiatric disorders. Therefore, it is important to understand how levels of BDNF are controlled. Recently we generated a novel transgenic mouse strain, termed the Bdnf-Luciferase transgenic (Bdnf-Luc Tg) mouse, to monitor changes in Bdnf expression. In the present study, we detected the bioluminescence signal from living Bdnf-Luc Tg mice after intraperitoneal administration of d-luciferin. Despite high levels of Bdnf expression in the brain, it was difficult to detect a signal from the brain region, probably because of its poorly penetrable (short-wavelength) bioluminescence. However, we could detect the changes in the bioluminescence signal in the brain region using a luciferin analogue generating a near-infrared wavelength of bioluminescence. We also found a strong correlation between increases in body weight and bioluminescence signal in the abdominal region of Tg mice fed a high-fat diet. These results show that changes in Bdnf expression can be visualized using living mice, and that the Tg mouse could be a powerful tool for clarification of the role of Bdnf expression in pathophysiological and physiological conditions.
format	article
author	Mamoru Fukuchi Hironori Izumi Hisashi Mori Masahiro Kiyama Satoshi Otsuka Shojiro Maki Yosuke Maehata Akiko Tabuchi Masaaki Tsuda
author_facet	Mamoru Fukuchi Hironori Izumi Hisashi Mori Masahiro Kiyama Satoshi Otsuka Shojiro Maki Yosuke Maehata Akiko Tabuchi Masaaki Tsuda
author_sort	Mamoru Fukuchi
title	Visualizing changes in brain-derived neurotrophic factor (BDNF) expression using bioluminescence imaging in living mice
title_short	Visualizing changes in brain-derived neurotrophic factor (BDNF) expression using bioluminescence imaging in living mice
title_full	Visualizing changes in brain-derived neurotrophic factor (BDNF) expression using bioluminescence imaging in living mice
title_fullStr	Visualizing changes in brain-derived neurotrophic factor (BDNF) expression using bioluminescence imaging in living mice
title_full_unstemmed	Visualizing changes in brain-derived neurotrophic factor (BDNF) expression using bioluminescence imaging in living mice
title_sort	visualizing changes in brain-derived neurotrophic factor (bdnf) expression using bioluminescence imaging in living mice
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b4141928f2b141788bb1cc12a0d39c29
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Visualizing changes in brain-derived neurotrophic factor (BDNF) expression using bioluminescence imaging in living mice

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