Measuring expression heterogeneity of single-cell cytoskeletal protein complexes

Existing methods for multimeric protein complex quantification in single cells suffer from limited selectivity and sensitivity. Here the authors report Single-cell protein Interaction Fractionation Through Electrophoresis and immunoassay Readout (SIFTER) and use this to probe the effects of cellular...

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Autores principales:	Julea Vlassakis, Louise L. Hansen, Ryo Higuchi-Sanabria, Yun Zhou, C. Kimberly Tsui, Andrew Dillin, Haiyan Huang, Amy E. Herr
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/b415092cbb764619a17faea6a93f2682
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spelling	oai:doaj.org-article:b415092cbb764619a17faea6a93f26822021-12-02T18:01:12ZMeasuring expression heterogeneity of single-cell cytoskeletal protein complexes10.1038/s41467-021-25212-32041-1723https://doaj.org/article/b415092cbb764619a17faea6a93f26822021-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-25212-3https://doaj.org/toc/2041-1723Existing methods for multimeric protein complex quantification in single cells suffer from limited selectivity and sensitivity. Here the authors report Single-cell protein Interaction Fractionation Through Electrophoresis and immunoassay Readout (SIFTER) and use this to probe the effects of cellular stress.Julea VlassakisLouise L. HansenRyo Higuchi-SanabriaYun ZhouC. Kimberly TsuiAndrew DillinHaiyan HuangAmy E. HerrNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Julea Vlassakis Louise L. Hansen Ryo Higuchi-Sanabria Yun Zhou C. Kimberly Tsui Andrew Dillin Haiyan Huang Amy E. Herr Measuring expression heterogeneity of single-cell cytoskeletal protein complexes
description	Existing methods for multimeric protein complex quantification in single cells suffer from limited selectivity and sensitivity. Here the authors report Single-cell protein Interaction Fractionation Through Electrophoresis and immunoassay Readout (SIFTER) and use this to probe the effects of cellular stress.
format	article
author	Julea Vlassakis Louise L. Hansen Ryo Higuchi-Sanabria Yun Zhou C. Kimberly Tsui Andrew Dillin Haiyan Huang Amy E. Herr
author_facet	Julea Vlassakis Louise L. Hansen Ryo Higuchi-Sanabria Yun Zhou C. Kimberly Tsui Andrew Dillin Haiyan Huang Amy E. Herr
author_sort	Julea Vlassakis
title	Measuring expression heterogeneity of single-cell cytoskeletal protein complexes
title_short	Measuring expression heterogeneity of single-cell cytoskeletal protein complexes
title_full	Measuring expression heterogeneity of single-cell cytoskeletal protein complexes
title_fullStr	Measuring expression heterogeneity of single-cell cytoskeletal protein complexes
title_full_unstemmed	Measuring expression heterogeneity of single-cell cytoskeletal protein complexes
title_sort	measuring expression heterogeneity of single-cell cytoskeletal protein complexes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b415092cbb764619a17faea6a93f2682
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Measuring expression heterogeneity of single-cell cytoskeletal protein complexes

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