Molecular diversity and relationships of fig associated nematodes from South Africa.

Nematodes of figs and fig wasps have received limited attention in Africa since their discovery in 1973. Sixteen of the 25 species of native South African figs were sampled for nematode associates using molecular barcoding with three loci (SSU, LSU D2-D3 and mtCOI) and fourteen (93%) were positive f...

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Autores principales:	Meike S Kruger, Natsumi Kanzaki, Robin M Giblin-Davis, Jaco M Greeff
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b41b2d24a51a4ebaaaf8ca03c3528fe9
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spelling	oai:doaj.org-article:b41b2d24a51a4ebaaaf8ca03c3528fe92021-12-02T20:18:27ZMolecular diversity and relationships of fig associated nematodes from South Africa.1932-620310.1371/journal.pone.0255451https://doaj.org/article/b41b2d24a51a4ebaaaf8ca03c3528fe92021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255451https://doaj.org/toc/1932-6203Nematodes of figs and fig wasps have received limited attention in Africa since their discovery in 1973. Sixteen of the 25 species of native South African figs were sampled for nematode associates using molecular barcoding with three loci (SSU, LSU D2-D3 and mtCOI) and fourteen (93%) were positive for at least one nematode species. Thirty-three putative species of nematodes were identified and classified according to the loci that were amplified and successfully sequenced. Fourteen putative nematode species were classified as Aphelenchoididae, of which nine were identified as Ficophagus from four species of Ficus from the section Galoglychia (i.e., five ex F. burkei including one shared with F. natalensis, one ex F. glumosa, one ex F. lutea, and one ex F. stuhlmannii) and one species ex F. sur from the section Sycomorus. In addition, there were four nematode species classified as Schistonchus s.s. from section Galoglychia figs (i.e., one ex F. burkei, two ex F. trichopoda, and one ex F. glumosa). There was also one species of Bursaphelenchus nematode recovered from F. sur from the section Sycomorus. Sixteen putative nematode species were classified as Diplogastridae, of which eight occurred in two clades of what is currently called Parasitodiplogaster with one (P. salicifoliae) being recovered from two Ficus species in the section Urostigma (F. salicifolia and F. ingens) and seven diplogastrids being associated with six species of Ficus from the section Galoglychia (i.e., two ex F. burkei including P. sycophilon, one ex F. stuhlmannii, one ex F. burtt-davyi, one ex F. trichopoda, one ex F. abutilifolia and one ex F. sansibarica). Three Acrostichus spp., a Teratodiplogaster and a Pristionchus species were recovered from F. sur and two Teratodiplogaster spp. and Pristionchus sycomori were recovered from F. sycomorus with both Ficus species belonging to the subgenus and section Sycomorus. The identities of the previously described T. martini and Parasitodiplogaster doliostoma (= Pristionchus sp. 35) are discussed. Lastly, there was a panagrolaimid identified from F. petersii.Meike S KrugerNatsumi KanzakiRobin M Giblin-DavisJaco M GreeffPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0255451 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Meike S Kruger Natsumi Kanzaki Robin M Giblin-Davis Jaco M Greeff Molecular diversity and relationships of fig associated nematodes from South Africa.
description	Nematodes of figs and fig wasps have received limited attention in Africa since their discovery in 1973. Sixteen of the 25 species of native South African figs were sampled for nematode associates using molecular barcoding with three loci (SSU, LSU D2-D3 and mtCOI) and fourteen (93%) were positive for at least one nematode species. Thirty-three putative species of nematodes were identified and classified according to the loci that were amplified and successfully sequenced. Fourteen putative nematode species were classified as Aphelenchoididae, of which nine were identified as Ficophagus from four species of Ficus from the section Galoglychia (i.e., five ex F. burkei including one shared with F. natalensis, one ex F. glumosa, one ex F. lutea, and one ex F. stuhlmannii) and one species ex F. sur from the section Sycomorus. In addition, there were four nematode species classified as Schistonchus s.s. from section Galoglychia figs (i.e., one ex F. burkei, two ex F. trichopoda, and one ex F. glumosa). There was also one species of Bursaphelenchus nematode recovered from F. sur from the section Sycomorus. Sixteen putative nematode species were classified as Diplogastridae, of which eight occurred in two clades of what is currently called Parasitodiplogaster with one (P. salicifoliae) being recovered from two Ficus species in the section Urostigma (F. salicifolia and F. ingens) and seven diplogastrids being associated with six species of Ficus from the section Galoglychia (i.e., two ex F. burkei including P. sycophilon, one ex F. stuhlmannii, one ex F. burtt-davyi, one ex F. trichopoda, one ex F. abutilifolia and one ex F. sansibarica). Three Acrostichus spp., a Teratodiplogaster and a Pristionchus species were recovered from F. sur and two Teratodiplogaster spp. and Pristionchus sycomori were recovered from F. sycomorus with both Ficus species belonging to the subgenus and section Sycomorus. The identities of the previously described T. martini and Parasitodiplogaster doliostoma (= Pristionchus sp. 35) are discussed. Lastly, there was a panagrolaimid identified from F. petersii.
format	article
author	Meike S Kruger Natsumi Kanzaki Robin M Giblin-Davis Jaco M Greeff
author_facet	Meike S Kruger Natsumi Kanzaki Robin M Giblin-Davis Jaco M Greeff
author_sort	Meike S Kruger
title	Molecular diversity and relationships of fig associated nematodes from South Africa.
title_short	Molecular diversity and relationships of fig associated nematodes from South Africa.
title_full	Molecular diversity and relationships of fig associated nematodes from South Africa.
title_fullStr	Molecular diversity and relationships of fig associated nematodes from South Africa.
title_full_unstemmed	Molecular diversity and relationships of fig associated nematodes from South Africa.
title_sort	molecular diversity and relationships of fig associated nematodes from south africa.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/b41b2d24a51a4ebaaaf8ca03c3528fe9
work_keys_str_mv	AT meikeskruger moleculardiversityandrelationshipsoffigassociatednematodesfromsouthafrica AT natsumikanzaki moleculardiversityandrelationshipsoffigassociatednematodesfromsouthafrica AT robinmgiblindavis moleculardiversityandrelationshipsoffigassociatednematodesfromsouthafrica AT jacomgreeff moleculardiversityandrelationshipsoffigassociatednematodesfromsouthafrica
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Molecular diversity and relationships of fig associated nematodes from South Africa.

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