Effect of particle size on phytochemical composition and antioxidant properties of Sargassum cristaefolium ethanol extract

Abstract Sample particle size is an important parameter in the solid–liquid extraction system of natural products for obtaining their bioactive compounds. This study evaluates the effect of sample particle size on the phytochemical composition and antioxidant activity of brown macroalgae Sargassum c...

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Autores principales:	E. S. Prasedya, A. Frediansyah, N. W. R. Martyasari, B. K. Ilhami, A. S. Abidin, H. Padmi, Fahrurrozi, A. B. Juanssilfero, S. Widyastuti, A. L. Sunarwidhi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b41b4810976c46adb253c3550f3e6023
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spelling	oai:doaj.org-article:b41b4810976c46adb253c3550f3e60232021-12-02T19:12:25ZEffect of particle size on phytochemical composition and antioxidant properties of Sargassum cristaefolium ethanol extract10.1038/s41598-021-95769-y2045-2322https://doaj.org/article/b41b4810976c46adb253c3550f3e60232021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95769-yhttps://doaj.org/toc/2045-2322Abstract Sample particle size is an important parameter in the solid–liquid extraction system of natural products for obtaining their bioactive compounds. This study evaluates the effect of sample particle size on the phytochemical composition and antioxidant activity of brown macroalgae Sargassum cristaefolium. The crude ethanol extract was extracted from dried powders of S.cristeafolium with various particle sizes (> 4000 µm, > 250 µm, > 125 µm, > 45 µm, and < 45 µm). The ethanolic extracts of S.cristaefolium were analysed for Total Phenolic Content (TPC), Total Flavonoid Content (TFC), phenolic compound concentration and antioxidant activities. The extract yield and phytochemical composition were more abundant in smaller particle sizes. Furthermore, the TPC (14.19 ± 2.08 mg GAE/g extract to 43.27 ± 2.56 mg GAE/g extract) and TFC (9.6 ± 1.8 mg QE/g extract to 70.27 ± 3.59 mg QE/g extract) values also significantly increased as particle sizes decreased. In addition, phenolic compounds epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC), and Epigallocatechin gallate (EGCG) concentration were frequently increased in samples of smaller particle sizes based on two-way ANOVA and Tukey’s multiple comparison analysis. These results correlate with the significantly stronger antioxidant activity in samples with smaller particle sizes. The smallest particle size (< 45 µm) demonstrated the strongest antioxidant activity based on DPPH, ABTS, hydroxyl assay and FRAP. In addition, ramp function graph evaluates the desired particle size for maximum phytochemical composition and antioxidant activity is 44 µm. In conclusion, current results show the importance of particle size reduction of macroalgae samples to increase the effectivity of its biological activity.E. S. PrasedyaA. FrediansyahN. W. R. MartyasariB. K. IlhamiA. S. AbidinH. PadmiFahrurroziA. B. JuanssilferoS. WidyastutiA. L. SunarwidhiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q E. S. Prasedya A. Frediansyah N. W. R. Martyasari B. K. Ilhami A. S. Abidin H. Padmi Fahrurrozi A. B. Juanssilfero S. Widyastuti A. L. Sunarwidhi Effect of particle size on phytochemical composition and antioxidant properties of Sargassum cristaefolium ethanol extract
description	Abstract Sample particle size is an important parameter in the solid–liquid extraction system of natural products for obtaining their bioactive compounds. This study evaluates the effect of sample particle size on the phytochemical composition and antioxidant activity of brown macroalgae Sargassum cristaefolium. The crude ethanol extract was extracted from dried powders of S.cristeafolium with various particle sizes (> 4000 µm, > 250 µm, > 125 µm, > 45 µm, and < 45 µm). The ethanolic extracts of S.cristaefolium were analysed for Total Phenolic Content (TPC), Total Flavonoid Content (TFC), phenolic compound concentration and antioxidant activities. The extract yield and phytochemical composition were more abundant in smaller particle sizes. Furthermore, the TPC (14.19 ± 2.08 mg GAE/g extract to 43.27 ± 2.56 mg GAE/g extract) and TFC (9.6 ± 1.8 mg QE/g extract to 70.27 ± 3.59 mg QE/g extract) values also significantly increased as particle sizes decreased. In addition, phenolic compounds epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC), and Epigallocatechin gallate (EGCG) concentration were frequently increased in samples of smaller particle sizes based on two-way ANOVA and Tukey’s multiple comparison analysis. These results correlate with the significantly stronger antioxidant activity in samples with smaller particle sizes. The smallest particle size (< 45 µm) demonstrated the strongest antioxidant activity based on DPPH, ABTS, hydroxyl assay and FRAP. In addition, ramp function graph evaluates the desired particle size for maximum phytochemical composition and antioxidant activity is 44 µm. In conclusion, current results show the importance of particle size reduction of macroalgae samples to increase the effectivity of its biological activity.
format	article
author	E. S. Prasedya A. Frediansyah N. W. R. Martyasari B. K. Ilhami A. S. Abidin H. Padmi Fahrurrozi A. B. Juanssilfero S. Widyastuti A. L. Sunarwidhi
author_facet	E. S. Prasedya A. Frediansyah N. W. R. Martyasari B. K. Ilhami A. S. Abidin H. Padmi Fahrurrozi A. B. Juanssilfero S. Widyastuti A. L. Sunarwidhi
author_sort	E. S. Prasedya
title	Effect of particle size on phytochemical composition and antioxidant properties of Sargassum cristaefolium ethanol extract
title_short	Effect of particle size on phytochemical composition and antioxidant properties of Sargassum cristaefolium ethanol extract
title_full	Effect of particle size on phytochemical composition and antioxidant properties of Sargassum cristaefolium ethanol extract
title_fullStr	Effect of particle size on phytochemical composition and antioxidant properties of Sargassum cristaefolium ethanol extract
title_full_unstemmed	Effect of particle size on phytochemical composition and antioxidant properties of Sargassum cristaefolium ethanol extract
title_sort	effect of particle size on phytochemical composition and antioxidant properties of sargassum cristaefolium ethanol extract
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b41b4810976c46adb253c3550f3e6023
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Effect of particle size on phytochemical composition and antioxidant properties of Sargassum cristaefolium ethanol extract

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