Multiplexed Single-Cell in situ RNA Profiling

The ability to quantify a large number of varied transcripts in single cells in their native spatial context is crucial to accelerate our understanding of health and disease. Bulk cell RNA analysis masks the heterogeneity in the cell population, while the conventional RNA imaging approaches suffer f...

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Autores principales:	Yu-Sheng Wang, Jia Guo
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	fluorescence in situ hybridization fish transcripts transcriptomics genomics Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/b42140a3f5ab48639d22ce319d40ab8c
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spelling	oai:doaj.org-article:b42140a3f5ab48639d22ce319d40ab8c2021-11-11T07:27:18ZMultiplexed Single-Cell in situ RNA Profiling2296-889X10.3389/fmolb.2021.775410https://doaj.org/article/b42140a3f5ab48639d22ce319d40ab8c2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmolb.2021.775410/fullhttps://doaj.org/toc/2296-889XThe ability to quantify a large number of varied transcripts in single cells in their native spatial context is crucial to accelerate our understanding of health and disease. Bulk cell RNA analysis masks the heterogeneity in the cell population, while the conventional RNA imaging approaches suffer from low multiplexing capacity. Recent advances in multiplexed fluorescence in situ hybridization (FISH) methods enable comprehensive RNA profiling in individual cells in situ. These technologies will have wide applications in many biological and biomedical fields, including cell type classification, signaling network analysis, tissue architecture, disease diagnosis and patient stratification, etc. In this minireview, we will present the recent technological advances of multiplexed single-cell in situ RNA profiling assays, discuss their advantages and limitations, describe their biological applications, highlight the current challenges, and propose potential solutions.Yu-Sheng WangJia GuoFrontiers Media S.A.articlefluorescence in situ hybridizationfishtranscriptstranscriptomicsgenomicsBiology (General)QH301-705.5ENFrontiers in Molecular Biosciences, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	fluorescence in situ hybridization fish transcripts transcriptomics genomics Biology (General) QH301-705.5
spellingShingle	fluorescence in situ hybridization fish transcripts transcriptomics genomics Biology (General) QH301-705.5 Yu-Sheng Wang Jia Guo Multiplexed Single-Cell in situ RNA Profiling
description	The ability to quantify a large number of varied transcripts in single cells in their native spatial context is crucial to accelerate our understanding of health and disease. Bulk cell RNA analysis masks the heterogeneity in the cell population, while the conventional RNA imaging approaches suffer from low multiplexing capacity. Recent advances in multiplexed fluorescence in situ hybridization (FISH) methods enable comprehensive RNA profiling in individual cells in situ. These technologies will have wide applications in many biological and biomedical fields, including cell type classification, signaling network analysis, tissue architecture, disease diagnosis and patient stratification, etc. In this minireview, we will present the recent technological advances of multiplexed single-cell in situ RNA profiling assays, discuss their advantages and limitations, describe their biological applications, highlight the current challenges, and propose potential solutions.
format	article
author	Yu-Sheng Wang Jia Guo
author_facet	Yu-Sheng Wang Jia Guo
author_sort	Yu-Sheng Wang
title	Multiplexed Single-Cell in situ RNA Profiling
title_short	Multiplexed Single-Cell in situ RNA Profiling
title_full	Multiplexed Single-Cell in situ RNA Profiling
title_fullStr	Multiplexed Single-Cell in situ RNA Profiling
title_full_unstemmed	Multiplexed Single-Cell in situ RNA Profiling
title_sort	multiplexed single-cell in situ rna profiling
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/b42140a3f5ab48639d22ce319d40ab8c
work_keys_str_mv	AT yushengwang multiplexedsinglecellinsiturnaprofiling AT jiaguo multiplexedsinglecellinsiturnaprofiling
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Multiplexed Single-Cell in situ RNA Profiling

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