High throughput viscoelastic particle focusing and separation in spiral microchannels

Abstract Passive particle manipulation using inertial and elasto-inertial microfluidics have received substantial interest in recent years and have found various applications in high throughput particle sorting and separation. For separation applications, elasto-inertial microfluidics has thus far b...

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Autores principales:	Tharagan Kumar, Harisha Ramachandraiah, Sharath Narayana Iyengar, Indradumna Banerjee, Gustaf Mårtensson, Aman Russom
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b42542d48d694074a4c61da6eb5eeea3
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spelling	oai:doaj.org-article:b42542d48d694074a4c61da6eb5eeea32021-12-02T15:27:06ZHigh throughput viscoelastic particle focusing and separation in spiral microchannels10.1038/s41598-021-88047-42045-2322https://doaj.org/article/b42542d48d694074a4c61da6eb5eeea32021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88047-4https://doaj.org/toc/2045-2322Abstract Passive particle manipulation using inertial and elasto-inertial microfluidics have received substantial interest in recent years and have found various applications in high throughput particle sorting and separation. For separation applications, elasto-inertial microfluidics has thus far been applied at substantial lower flow rates as compared to inertial microfluidics. In this work, we explore viscoelastic particle focusing and separation in spiral channels at two orders of magnitude higher Reynolds numbers than previously reported. We show that the balance between dominant inertial lift force, dean drag force and elastic force enables stable 3D particle focusing at dynamically high Reynolds numbers. Using a two-turn spiral, we show that particles, initially pinched towards the inner wall using an elasticity enhancer, PEO (polyethylene oxide), as sheath migrate towards the outer wall strictly based on size and can be effectively separated with high precision. As a proof of principle for high resolution particle separation, 15 µm particles were effectively separated from 10 µm particles. A separation efficiency of 98% for the 10 µm and 97% for the 15 µm particles was achieved. Furthermore, we demonstrate sheath-less, high throughput, separation using a novel integrated two-spiral device and achieved a separation efficiency of 89% for the 10 µm and 99% for the 15 µm particles at a sample flow rate of 1 mL/min—a throughput previously only reported for inertial microfluidics. We anticipate the ability to precisely control particles in 3D at extremely high flow rates will open up several applications, including the development of ultra-high throughput microflow cytometers and high-resolution separation of rare cells for point of care diagnostics.Tharagan KumarHarisha RamachandraiahSharath Narayana IyengarIndradumna BanerjeeGustaf MårtenssonAman RussomNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tharagan Kumar Harisha Ramachandraiah Sharath Narayana Iyengar Indradumna Banerjee Gustaf Mårtensson Aman Russom High throughput viscoelastic particle focusing and separation in spiral microchannels
description	Abstract Passive particle manipulation using inertial and elasto-inertial microfluidics have received substantial interest in recent years and have found various applications in high throughput particle sorting and separation. For separation applications, elasto-inertial microfluidics has thus far been applied at substantial lower flow rates as compared to inertial microfluidics. In this work, we explore viscoelastic particle focusing and separation in spiral channels at two orders of magnitude higher Reynolds numbers than previously reported. We show that the balance between dominant inertial lift force, dean drag force and elastic force enables stable 3D particle focusing at dynamically high Reynolds numbers. Using a two-turn spiral, we show that particles, initially pinched towards the inner wall using an elasticity enhancer, PEO (polyethylene oxide), as sheath migrate towards the outer wall strictly based on size and can be effectively separated with high precision. As a proof of principle for high resolution particle separation, 15 µm particles were effectively separated from 10 µm particles. A separation efficiency of 98% for the 10 µm and 97% for the 15 µm particles was achieved. Furthermore, we demonstrate sheath-less, high throughput, separation using a novel integrated two-spiral device and achieved a separation efficiency of 89% for the 10 µm and 99% for the 15 µm particles at a sample flow rate of 1 mL/min—a throughput previously only reported for inertial microfluidics. We anticipate the ability to precisely control particles in 3D at extremely high flow rates will open up several applications, including the development of ultra-high throughput microflow cytometers and high-resolution separation of rare cells for point of care diagnostics.
format	article
author	Tharagan Kumar Harisha Ramachandraiah Sharath Narayana Iyengar Indradumna Banerjee Gustaf Mårtensson Aman Russom
author_facet	Tharagan Kumar Harisha Ramachandraiah Sharath Narayana Iyengar Indradumna Banerjee Gustaf Mårtensson Aman Russom
author_sort	Tharagan Kumar
title	High throughput viscoelastic particle focusing and separation in spiral microchannels
title_short	High throughput viscoelastic particle focusing and separation in spiral microchannels
title_full	High throughput viscoelastic particle focusing and separation in spiral microchannels
title_fullStr	High throughput viscoelastic particle focusing and separation in spiral microchannels
title_full_unstemmed	High throughput viscoelastic particle focusing and separation in spiral microchannels
title_sort	high throughput viscoelastic particle focusing and separation in spiral microchannels
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b42542d48d694074a4c61da6eb5eeea3
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High throughput viscoelastic particle focusing and separation in spiral microchannels

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