Biotechnology Application in Wood Industry: Utilization of Phenol Oxidase Enzyme as a Renewable Binder in MDF Manufacture

Enzymatic activation of lignin on pinewood TMP (Thermo-Mechanical Pulp) fibers for MDF production was investigated using electronic microscopic technique. Two systems: laccase mediator and laccase without mediator were used. MDF was made with laccase as biological catalysts in both wet and dry proce...

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Autor principal:	Alireza Kharazipoor
Formato:	article
Lenguaje:	FA
Publicado:	Regional Information Center for Science and Technology (RICeST) 2012
Materias:	Enzymatic activation Thermo-Mechanical Pulp Hydroxy benzoic acid phenol oxidase enzymes Forestry SD1-669.5
Acceso en línea:	https://doaj.org/article/b42cf400a7584a5c9e6985c7a422dd4e
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id	oai:doaj.org-article:b42cf400a7584a5c9e6985c7a422dd4e
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spelling	oai:doaj.org-article:b42cf400a7584a5c9e6985c7a422dd4e2021-12-02T03:52:42ZBiotechnology Application in Wood Industry: Utilization of Phenol Oxidase Enzyme as a Renewable Binder in MDF Manufacture1735-09132383-112X10.22092/ijwpr.2012.117067https://doaj.org/article/b42cf400a7584a5c9e6985c7a422dd4e2012-06-01T00:00:00Zhttp://ijwpr.areeo.ac.ir/article_117067_6b8bce6977525c44244b08fbbfc42b68.pdfhttps://doaj.org/toc/1735-0913https://doaj.org/toc/2383-112XEnzymatic activation of lignin on pinewood TMP (Thermo-Mechanical Pulp) fibers for MDF production was investigated using electronic microscopic technique. Two systems: laccase mediator and laccase without mediator were used. MDF was made with laccase as biological catalysts in both wet and dry process. Incubation of fibers with commercially available Trametes versicolor laccase was performed in aqueous solution (wet process) or the enzyme was sprayed onto the fiber (dry process). Both laccase mediator and laccase without mediator imparted major impact on the optimization of physico-mechanical properties to achieve minimum requirement of standard. Boards produced with fiber-treated with laccase were found to have higher mechanical strength than non-treated binder-free control boards. Best board properties (bending strength, internal bond strength and swelling) were observed after an incubation of 10-15 min at pH 5.6 and a concentration of 100 U/ml laccase with a mediator (4- Hydroxy benzoic acid 98%) concentration of 10 mM added at 3 min intervals . Electron-microscopic micrographs of enzyme-bonded fibers showed that the application of mediator enhances laccase reaction and, most importantly, the substrate spectrum of the enzymes broadens dramatically. Laccase (phenol oxidase enzymes) serves as an activator of lignin by radical production. Certainly it can be deduced from these studies, that enzymatic treatment altered the surface properties of the fibers. Laccase mediator treated fibers showed a marked increase in the hydrophobicity.Alireza KharazipoorRegional Information Center for Science and Technology (RICeST)articleEnzymatic activationThermo-Mechanical PulpHydroxy benzoic acidphenol oxidase enzymesForestrySD1-669.5FAتحقیقات علوم چوب و کاغذ ایران, Vol 27, Iss 2, Pp 275-293 (2012)
institution	DOAJ
collection	DOAJ
language	FA
topic	Enzymatic activation Thermo-Mechanical Pulp Hydroxy benzoic acid phenol oxidase enzymes Forestry SD1-669.5
spellingShingle	Enzymatic activation Thermo-Mechanical Pulp Hydroxy benzoic acid phenol oxidase enzymes Forestry SD1-669.5 Alireza Kharazipoor Biotechnology Application in Wood Industry: Utilization of Phenol Oxidase Enzyme as a Renewable Binder in MDF Manufacture
description	Enzymatic activation of lignin on pinewood TMP (Thermo-Mechanical Pulp) fibers for MDF production was investigated using electronic microscopic technique. Two systems: laccase mediator and laccase without mediator were used. MDF was made with laccase as biological catalysts in both wet and dry process. Incubation of fibers with commercially available Trametes versicolor laccase was performed in aqueous solution (wet process) or the enzyme was sprayed onto the fiber (dry process). Both laccase mediator and laccase without mediator imparted major impact on the optimization of physico-mechanical properties to achieve minimum requirement of standard. Boards produced with fiber-treated with laccase were found to have higher mechanical strength than non-treated binder-free control boards. Best board properties (bending strength, internal bond strength and swelling) were observed after an incubation of 10-15 min at pH 5.6 and a concentration of 100 U/ml laccase with a mediator (4- Hydroxy benzoic acid 98%) concentration of 10 mM added at 3 min intervals . Electron-microscopic micrographs of enzyme-bonded fibers showed that the application of mediator enhances laccase reaction and, most importantly, the substrate spectrum of the enzymes broadens dramatically. Laccase (phenol oxidase enzymes) serves as an activator of lignin by radical production. Certainly it can be deduced from these studies, that enzymatic treatment altered the surface properties of the fibers. Laccase mediator treated fibers showed a marked increase in the hydrophobicity.
format	article
author	Alireza Kharazipoor
author_facet	Alireza Kharazipoor
author_sort	Alireza Kharazipoor
title	Biotechnology Application in Wood Industry: Utilization of Phenol Oxidase Enzyme as a Renewable Binder in MDF Manufacture
title_short	Biotechnology Application in Wood Industry: Utilization of Phenol Oxidase Enzyme as a Renewable Binder in MDF Manufacture
title_full	Biotechnology Application in Wood Industry: Utilization of Phenol Oxidase Enzyme as a Renewable Binder in MDF Manufacture
title_fullStr	Biotechnology Application in Wood Industry: Utilization of Phenol Oxidase Enzyme as a Renewable Binder in MDF Manufacture
title_full_unstemmed	Biotechnology Application in Wood Industry: Utilization of Phenol Oxidase Enzyme as a Renewable Binder in MDF Manufacture
title_sort	biotechnology application in wood industry: utilization of phenol oxidase enzyme as a renewable binder in mdf manufacture
publisher	Regional Information Center for Science and Technology (RICeST)
publishDate	2012
url	https://doaj.org/article/b42cf400a7584a5c9e6985c7a422dd4e
work_keys_str_mv	AT alirezakharazipoor biotechnologyapplicationinwoodindustryutilizationofphenoloxidaseenzymeasarenewablebinderinmdfmanufacture
_version_	1718401575045562368

Biotechnology Application in Wood Industry: Utilization of Phenol Oxidase Enzyme as a Renewable Binder in MDF Manufacture

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