Immunohistological Localization of Mel1a Melatonin Receptor in Pigeon Retina

Wenlong Sheng,1 Shijun Weng,2 Fei Li,1 Yun Zhang,1 Qiuxia He,1 Wenxiang Sheng,1 Ying Fu,3 Haiyue Yan,4 Kechun Liu1 1Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, People’s Republic of China; 2State Key Laboratory of Medical Neurobiology, Institutes...

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Autores principales:	Sheng W, Weng S, Li F, Zhang Y, He Q, Fu Y, Yan H, Liu K
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	circadian rhythm retinal mel1a receptor cellular localization diurnal animal. Psychiatry RC435-571 Neurophysiology and neuropsychology QP351-495
Acceso en línea:	https://doaj.org/article/b42d7dbfb32b4903a568102d6c7af49f
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spelling	oai:doaj.org-article:b42d7dbfb32b4903a568102d6c7af49f2021-12-02T13:30:38ZImmunohistological Localization of Mel1a Melatonin Receptor in Pigeon Retina1179-1608https://doaj.org/article/b42d7dbfb32b4903a568102d6c7af49f2021-02-01T00:00:00Zhttps://www.dovepress.com/immunohistological-localization-of-mel1a-melatonin-receptor-in-pigeon--peer-reviewed-article-NSShttps://doaj.org/toc/1179-1608Wenlong Sheng,1 Shijun Weng,2 Fei Li,1 Yun Zhang,1 Qiuxia He,1 Wenxiang Sheng,1 Ying Fu,3 Haiyue Yan,4 Kechun Liu1 1Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, People’s Republic of China; 2State Key Laboratory of Medical Neurobiology, Institutes of Brain Science, Department of Ophthalmology, Zhongshan Hospital, Fudan University, Shanghai, People’s Republic of China; 3Shandong Science and Technology Exchange Center, Jinan, People’s Republic of China; 4Shandong Institute of Scientific and Technical Information, Jinan, People’s Republic of ChinaCorrespondence: Wenlong Sheng; Kechun Liu Email shengwenlong1121@163.com; liukechun2000@163.comBackground: Melatonin (N-acetyl-5-methoxytryptamine), a significant indoleamine neuromodulator implicated in circadian rhythms and sleep patterns, regulates diverse rhythmic functions via activating its high-affinity G-protein-coupled receptors. However, the detailed cellular expression of the Mel1a receptor in the retina is still a research gap.Methods: The expression of the Mel1a receptor in pigeon retina was assessed using Western blot analysis and immunofluorescent staining. The cellular localization of the Mel1a receptor was studied using double immunofluorescent staining and laser-scanning confocal microscopy.Results: Our data suggested that the Mel1a receptor was extensively expressed in the outer segment of Rho4D2-labeled rod and L/M-opsin-labeled red/green cone and in the somata of the CB-labeled horizontal cell, TH-labeled dopaminergic amacrine cell, ChAT-labeled cholinergic amacrine cell, PV-labeled AII amacrine cell, Brn3a-labeled conventional ganglion cell, melanopsin-containing ganglion cell and CRALBP-labeled Müller glial cell. In addition, the Mel1a receptor was diffusely distributed throughout the full thickness of the inner plexiform layer. However, the outer segment of S-opsin-labeled blue cone, the somata of ChX-10-labeled bipolar cell and outer plexiform layer seemed to lack immunoreactivity of the Mel1a receptor.Conclusion: The finding that multiple types of retinal cells express the Mel1a receptor provides a new neurobiological basis for the participation of melatonin in the regulation of retinal functions through activating the Mel1a receptor.Keywords: circadian rhythm, retinal Mel1a receptor, cellular localization, diurnal animalSheng WWeng SLi FZhang YHe QSheng WFu YYan HLiu KDove Medical Pressarticlecircadian rhythmretinal mel1a receptorcellular localizationdiurnal animal.PsychiatryRC435-571Neurophysiology and neuropsychologyQP351-495ENNature and Science of Sleep, Vol Volume 13, Pp 113-121 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	circadian rhythm retinal mel1a receptor cellular localization diurnal animal. Psychiatry RC435-571 Neurophysiology and neuropsychology QP351-495
spellingShingle	circadian rhythm retinal mel1a receptor cellular localization diurnal animal. Psychiatry RC435-571 Neurophysiology and neuropsychology QP351-495 Sheng W Weng S Li F Zhang Y He Q Sheng W Fu Y Yan H Liu K Immunohistological Localization of Mel1a Melatonin Receptor in Pigeon Retina
description	Wenlong Sheng,1 Shijun Weng,2 Fei Li,1 Yun Zhang,1 Qiuxia He,1 Wenxiang Sheng,1 Ying Fu,3 Haiyue Yan,4 Kechun Liu1 1Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, People’s Republic of China; 2State Key Laboratory of Medical Neurobiology, Institutes of Brain Science, Department of Ophthalmology, Zhongshan Hospital, Fudan University, Shanghai, People’s Republic of China; 3Shandong Science and Technology Exchange Center, Jinan, People’s Republic of China; 4Shandong Institute of Scientific and Technical Information, Jinan, People’s Republic of ChinaCorrespondence: Wenlong Sheng; Kechun Liu Email shengwenlong1121@163.com; liukechun2000@163.comBackground: Melatonin (N-acetyl-5-methoxytryptamine), a significant indoleamine neuromodulator implicated in circadian rhythms and sleep patterns, regulates diverse rhythmic functions via activating its high-affinity G-protein-coupled receptors. However, the detailed cellular expression of the Mel1a receptor in the retina is still a research gap.Methods: The expression of the Mel1a receptor in pigeon retina was assessed using Western blot analysis and immunofluorescent staining. The cellular localization of the Mel1a receptor was studied using double immunofluorescent staining and laser-scanning confocal microscopy.Results: Our data suggested that the Mel1a receptor was extensively expressed in the outer segment of Rho4D2-labeled rod and L/M-opsin-labeled red/green cone and in the somata of the CB-labeled horizontal cell, TH-labeled dopaminergic amacrine cell, ChAT-labeled cholinergic amacrine cell, PV-labeled AII amacrine cell, Brn3a-labeled conventional ganglion cell, melanopsin-containing ganglion cell and CRALBP-labeled Müller glial cell. In addition, the Mel1a receptor was diffusely distributed throughout the full thickness of the inner plexiform layer. However, the outer segment of S-opsin-labeled blue cone, the somata of ChX-10-labeled bipolar cell and outer plexiform layer seemed to lack immunoreactivity of the Mel1a receptor.Conclusion: The finding that multiple types of retinal cells express the Mel1a receptor provides a new neurobiological basis for the participation of melatonin in the regulation of retinal functions through activating the Mel1a receptor.Keywords: circadian rhythm, retinal Mel1a receptor, cellular localization, diurnal animal
format	article
author	Sheng W Weng S Li F Zhang Y He Q Sheng W Fu Y Yan H Liu K
author_facet	Sheng W Weng S Li F Zhang Y He Q Sheng W Fu Y Yan H Liu K
author_sort	Sheng W
title	Immunohistological Localization of Mel1a Melatonin Receptor in Pigeon Retina
title_short	Immunohistological Localization of Mel1a Melatonin Receptor in Pigeon Retina
title_full	Immunohistological Localization of Mel1a Melatonin Receptor in Pigeon Retina
title_fullStr	Immunohistological Localization of Mel1a Melatonin Receptor in Pigeon Retina
title_full_unstemmed	Immunohistological Localization of Mel1a Melatonin Receptor in Pigeon Retina
title_sort	immunohistological localization of mel1a melatonin receptor in pigeon retina
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/b42d7dbfb32b4903a568102d6c7af49f
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Immunohistological Localization of Mel1a Melatonin Receptor in Pigeon Retina

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