Cat amniotic membrane multipotent cells are nontumorigenic and are safe for use in cell transplantation

Atanasio S Vidane,1 Aline F Souza,1 Rafael V Sampaio,1 Fabiana F Bressan,2 Naira C Pieri,1 Daniele S Martins,2 Flavio V Meirelles,2 Maria A Miglino,1 Carlos E Ambrósio2 1Department of Surgery, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, S&at...

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Autores principales:	Vidane AS, Souza AF, Sampaio RV, Bressan FF, Pieri NC, Martins DS, Meirelles FV, Miglino MA, Ambrósio CE
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Cytology QH573-671
Acceso en línea:	https://doaj.org/article/b430975f37cb410187361e596fbd8f00
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spelling	oai:doaj.org-article:b430975f37cb410187361e596fbd8f002021-12-02T02:52:56ZCat amniotic membrane multipotent cells are nontumorigenic and are safe for use in cell transplantation1178-6957https://doaj.org/article/b430975f37cb410187361e596fbd8f002014-08-01T00:00:00Zhttp://www.dovepress.com/cat-amniotic-membrane-multipotent-cells-are-nontumorigenic-and-are-saf-peer-reviewed-article-SCCAAhttps://doaj.org/toc/1178-6957 Atanasio S Vidane,1 Aline F Souza,1 Rafael V Sampaio,1 Fabiana F Bressan,2 Naira C Pieri,1 Daniele S Martins,2 Flavio V Meirelles,2 Maria A Miglino,1 Carlos E Ambrósio2 1Department of Surgery, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil; 2Department of Veterinary Medicine, Faculty of Animal Sciences and Food Engineering, University of São Paulo, São Paulo, Brazil Abstract: Amnion-derived mesenchymal stem cells (AMSCs) are multipotent cells with an enhanced ability to differentiate into multiple lineages. AMSCs can be acquired through noninvasive methods, and therefore are exempt from the typical ethical issues surrounding stem cell use. The objective of this study was to isolate and characterize AMSCs from a cat amniotic membrane for future application in regenerative medicine. The cat AMSCs were harvested after mechanical and enzymatic digestion of amnion. In culture medium, the cat AMSCs adhered to a plastic culture dish and displayed a fibroblast-like morphology. Immunophenotyping assays were positive for the mesenchymal stem cell-specific markers CD73 and CD90 but not the hematopoietic markers CD34, CD45, and CD79. Under appropriate conditions, the cat AMSCs differentiated into osteogenic, chondrogenic, and adipogenic cell lineages. One advantage of cat AMSCs was nonteratogenicity, assessed 4 weeks post injection of undifferentiated AMSCs into immunodeficient mice. These findings suggest that cat amniotic membranes may be an important and useful source of mesenchymal stem cells for clinical applications, especially for cell or tissue replacement in chronic and degenerative diseases. Keywords: amnion, cats, cell differentiation, fetal membranes, mesenchymal cellsVidane ASSouza AFSampaio RVBressan FFPieri NCMartins DSMeirelles FVMiglino MAAmbrósio CEDove Medical PressarticleCytologyQH573-671ENStem Cells and Cloning: Advances and Applications, Vol 2014, Iss default, Pp 71-78 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cytology QH573-671
spellingShingle	Cytology QH573-671 Vidane AS Souza AF Sampaio RV Bressan FF Pieri NC Martins DS Meirelles FV Miglino MA Ambrósio CE Cat amniotic membrane multipotent cells are nontumorigenic and are safe for use in cell transplantation
description	Atanasio S Vidane,1 Aline F Souza,1 Rafael V Sampaio,1 Fabiana F Bressan,2 Naira C Pieri,1 Daniele S Martins,2 Flavio V Meirelles,2 Maria A Miglino,1 Carlos E Ambrósio2 1Department of Surgery, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil; 2Department of Veterinary Medicine, Faculty of Animal Sciences and Food Engineering, University of São Paulo, São Paulo, Brazil Abstract: Amnion-derived mesenchymal stem cells (AMSCs) are multipotent cells with an enhanced ability to differentiate into multiple lineages. AMSCs can be acquired through noninvasive methods, and therefore are exempt from the typical ethical issues surrounding stem cell use. The objective of this study was to isolate and characterize AMSCs from a cat amniotic membrane for future application in regenerative medicine. The cat AMSCs were harvested after mechanical and enzymatic digestion of amnion. In culture medium, the cat AMSCs adhered to a plastic culture dish and displayed a fibroblast-like morphology. Immunophenotyping assays were positive for the mesenchymal stem cell-specific markers CD73 and CD90 but not the hematopoietic markers CD34, CD45, and CD79. Under appropriate conditions, the cat AMSCs differentiated into osteogenic, chondrogenic, and adipogenic cell lineages. One advantage of cat AMSCs was nonteratogenicity, assessed 4 weeks post injection of undifferentiated AMSCs into immunodeficient mice. These findings suggest that cat amniotic membranes may be an important and useful source of mesenchymal stem cells for clinical applications, especially for cell or tissue replacement in chronic and degenerative diseases. Keywords: amnion, cats, cell differentiation, fetal membranes, mesenchymal cells
format	article
author	Vidane AS Souza AF Sampaio RV Bressan FF Pieri NC Martins DS Meirelles FV Miglino MA Ambrósio CE
author_facet	Vidane AS Souza AF Sampaio RV Bressan FF Pieri NC Martins DS Meirelles FV Miglino MA Ambrósio CE
author_sort	Vidane AS
title	Cat amniotic membrane multipotent cells are nontumorigenic and are safe for use in cell transplantation
title_short	Cat amniotic membrane multipotent cells are nontumorigenic and are safe for use in cell transplantation
title_full	Cat amniotic membrane multipotent cells are nontumorigenic and are safe for use in cell transplantation
title_fullStr	Cat amniotic membrane multipotent cells are nontumorigenic and are safe for use in cell transplantation
title_full_unstemmed	Cat amniotic membrane multipotent cells are nontumorigenic and are safe for use in cell transplantation
title_sort	cat amniotic membrane multipotent cells are nontumorigenic and are safe for use in cell transplantation
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/b430975f37cb410187361e596fbd8f00
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Cat amniotic membrane multipotent cells are nontumorigenic and are safe for use in cell transplantation

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