Comprehensive genetic analysis of early host body reactions to the bioactive and bio-inert porous scaffolds.

To design scaffolds for tissue regeneration, details of the host body reaction to the scaffolds must be studied. Host body reactions have been investigated mainly by immunohistological observations for a long time. Despite of recent dramatic development in genetic analysis technologies, genetically...

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Autores principales:	Tomo Ehashi, Taro Takemura, Nobutaka Hanagata, Takashi Minowa, Hisatoshi Kobayashi, Kazuhiko Ishihara, Tetsuji Yamaoka
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b451923691db4e71939778df58bc98b4
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spelling	oai:doaj.org-article:b451923691db4e71939778df58bc98b42021-11-18T08:37:51ZComprehensive genetic analysis of early host body reactions to the bioactive and bio-inert porous scaffolds.1932-620310.1371/journal.pone.0085132https://doaj.org/article/b451923691db4e71939778df58bc98b42014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24454803/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203To design scaffolds for tissue regeneration, details of the host body reaction to the scaffolds must be studied. Host body reactions have been investigated mainly by immunohistological observations for a long time. Despite of recent dramatic development in genetic analysis technologies, genetically comprehensive changes in host body reactions are hardly studied. There is no information about host body reactions that can predict successful tissue regeneration in the future. In the present study, porous polyethylene scaffolds were coated with bioactive collagen or bio-inert poly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate) (PMB) and were implanted subcutaneously and compared the host body reaction to those substrates by normalizing the result using control non-coat polyethylene scaffold. The comprehensive analyses of early host body reactions to the scaffolds were carried out using a DNA microarray assay. Within numerous genes which were expressed differently among these scaffolds, particular genes related to inflammation, wound healing, and angiogenesis were focused upon. Interleukin (IL)-1β and IL-10 are important cytokines in tissue responses to biomaterials because IL-1β promotes both inflammation and wound healing and IL-10 suppresses both of them. IL-1β was up-regulated in the collagen-coated scaffold. Collagen-specifically up-regulated genes contained both M1- and M2-macrophage-related genes. Marked vessel formation in the collagen-coated scaffold was occurred in accordance with the up-regulation of many angiogenesis-inducible factors. The DNA microarray assay provided global information regarding the host body reaction. Interestingly, several up-regulated genes were detected even on the very bio-inert PMB-coated surfaces and those genes include inflammation-suppressive and wound healing-suppressive IL-10, suggesting that not only active tissue response but also the inert response may relates to these genetic regulations.Tomo EhashiTaro TakemuraNobutaka HanagataTakashi MinowaHisatoshi KobayashiKazuhiko IshiharaTetsuji YamaokaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e85132 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tomo Ehashi Taro Takemura Nobutaka Hanagata Takashi Minowa Hisatoshi Kobayashi Kazuhiko Ishihara Tetsuji Yamaoka Comprehensive genetic analysis of early host body reactions to the bioactive and bio-inert porous scaffolds.
description	To design scaffolds for tissue regeneration, details of the host body reaction to the scaffolds must be studied. Host body reactions have been investigated mainly by immunohistological observations for a long time. Despite of recent dramatic development in genetic analysis technologies, genetically comprehensive changes in host body reactions are hardly studied. There is no information about host body reactions that can predict successful tissue regeneration in the future. In the present study, porous polyethylene scaffolds were coated with bioactive collagen or bio-inert poly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate) (PMB) and were implanted subcutaneously and compared the host body reaction to those substrates by normalizing the result using control non-coat polyethylene scaffold. The comprehensive analyses of early host body reactions to the scaffolds were carried out using a DNA microarray assay. Within numerous genes which were expressed differently among these scaffolds, particular genes related to inflammation, wound healing, and angiogenesis were focused upon. Interleukin (IL)-1β and IL-10 are important cytokines in tissue responses to biomaterials because IL-1β promotes both inflammation and wound healing and IL-10 suppresses both of them. IL-1β was up-regulated in the collagen-coated scaffold. Collagen-specifically up-regulated genes contained both M1- and M2-macrophage-related genes. Marked vessel formation in the collagen-coated scaffold was occurred in accordance with the up-regulation of many angiogenesis-inducible factors. The DNA microarray assay provided global information regarding the host body reaction. Interestingly, several up-regulated genes were detected even on the very bio-inert PMB-coated surfaces and those genes include inflammation-suppressive and wound healing-suppressive IL-10, suggesting that not only active tissue response but also the inert response may relates to these genetic regulations.
format	article
author	Tomo Ehashi Taro Takemura Nobutaka Hanagata Takashi Minowa Hisatoshi Kobayashi Kazuhiko Ishihara Tetsuji Yamaoka
author_facet	Tomo Ehashi Taro Takemura Nobutaka Hanagata Takashi Minowa Hisatoshi Kobayashi Kazuhiko Ishihara Tetsuji Yamaoka
author_sort	Tomo Ehashi
title	Comprehensive genetic analysis of early host body reactions to the bioactive and bio-inert porous scaffolds.
title_short	Comprehensive genetic analysis of early host body reactions to the bioactive and bio-inert porous scaffolds.
title_full	Comprehensive genetic analysis of early host body reactions to the bioactive and bio-inert porous scaffolds.
title_fullStr	Comprehensive genetic analysis of early host body reactions to the bioactive and bio-inert porous scaffolds.
title_full_unstemmed	Comprehensive genetic analysis of early host body reactions to the bioactive and bio-inert porous scaffolds.
title_sort	comprehensive genetic analysis of early host body reactions to the bioactive and bio-inert porous scaffolds.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/b451923691db4e71939778df58bc98b4
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Comprehensive genetic analysis of early host body reactions to the bioactive and bio-inert porous scaffolds.

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