Physiological and metabolomic consequences of reduced expression of the Drosophila brummer triglyceride Lipase.

Disruption of lipolysis has widespread effects on intermediary metabolism and organismal phenotypes. Defects in lipolysis can be modeled in Drosophila melanogaster through genetic manipulations of brummer (bmm), which encodes a triglyceride lipase orthologous to mammalian Adipose Triglyceride Lipase...

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Autores principales:	Nestor O Nazario-Yepiz, Jaime Fernández Sobaberas, Roberta Lyman, Marion R Campbell, Vijay Shankar, Robert R H Anholt, Trudy F C Mackay
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b451a74a50774c0fb3146510816e9adc
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spelling	oai:doaj.org-article:b451a74a50774c0fb3146510816e9adc2021-12-02T20:14:27ZPhysiological and metabolomic consequences of reduced expression of the Drosophila brummer triglyceride Lipase.1932-620310.1371/journal.pone.0255198https://doaj.org/article/b451a74a50774c0fb3146510816e9adc2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255198https://doaj.org/toc/1932-6203Disruption of lipolysis has widespread effects on intermediary metabolism and organismal phenotypes. Defects in lipolysis can be modeled in Drosophila melanogaster through genetic manipulations of brummer (bmm), which encodes a triglyceride lipase orthologous to mammalian Adipose Triglyceride Lipase. RNAi-mediated knock-down of bmm in all tissues or metabolic specific tissues results in reduced locomotor activity, altered sleep patterns and reduced lifespan. Metabolomic analysis on flies in which bmm is downregulated reveals a marked reduction in medium chain fatty acids, long chain saturated fatty acids and long chain monounsaturated and polyunsaturated fatty acids, and an increase in diacylglycerol levels. Elevated carbohydrate metabolites and tricarboxylic acid intermediates indicate that impairment of fatty acid mobilization as an energy source may result in upregulation of compensatory carbohydrate catabolism. bmm downregulation also results in elevated levels of serotonin and dopamine neurotransmitters, possibly accounting for the impairment of locomotor activity and sleep patterns. Physiological phenotypes and metabolomic changes upon reduction of bmm expression show extensive sexual dimorphism. Altered metabolic states in the Drosophila model are relevant for understanding human metabolic disorders, since pathways of intermediary metabolism are conserved across phyla.Nestor O Nazario-YepizJaime Fernández SobaberasRoberta LymanMarion R CampbellVijay ShankarRobert R H AnholtTrudy F C MackayPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0255198 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nestor O Nazario-Yepiz Jaime Fernández Sobaberas Roberta Lyman Marion R Campbell Vijay Shankar Robert R H Anholt Trudy F C Mackay Physiological and metabolomic consequences of reduced expression of the Drosophila brummer triglyceride Lipase.
description	Disruption of lipolysis has widespread effects on intermediary metabolism and organismal phenotypes. Defects in lipolysis can be modeled in Drosophila melanogaster through genetic manipulations of brummer (bmm), which encodes a triglyceride lipase orthologous to mammalian Adipose Triglyceride Lipase. RNAi-mediated knock-down of bmm in all tissues or metabolic specific tissues results in reduced locomotor activity, altered sleep patterns and reduced lifespan. Metabolomic analysis on flies in which bmm is downregulated reveals a marked reduction in medium chain fatty acids, long chain saturated fatty acids and long chain monounsaturated and polyunsaturated fatty acids, and an increase in diacylglycerol levels. Elevated carbohydrate metabolites and tricarboxylic acid intermediates indicate that impairment of fatty acid mobilization as an energy source may result in upregulation of compensatory carbohydrate catabolism. bmm downregulation also results in elevated levels of serotonin and dopamine neurotransmitters, possibly accounting for the impairment of locomotor activity and sleep patterns. Physiological phenotypes and metabolomic changes upon reduction of bmm expression show extensive sexual dimorphism. Altered metabolic states in the Drosophila model are relevant for understanding human metabolic disorders, since pathways of intermediary metabolism are conserved across phyla.
format	article
author	Nestor O Nazario-Yepiz Jaime Fernández Sobaberas Roberta Lyman Marion R Campbell Vijay Shankar Robert R H Anholt Trudy F C Mackay
author_facet	Nestor O Nazario-Yepiz Jaime Fernández Sobaberas Roberta Lyman Marion R Campbell Vijay Shankar Robert R H Anholt Trudy F C Mackay
author_sort	Nestor O Nazario-Yepiz
title	Physiological and metabolomic consequences of reduced expression of the Drosophila brummer triglyceride Lipase.
title_short	Physiological and metabolomic consequences of reduced expression of the Drosophila brummer triglyceride Lipase.
title_full	Physiological and metabolomic consequences of reduced expression of the Drosophila brummer triglyceride Lipase.
title_fullStr	Physiological and metabolomic consequences of reduced expression of the Drosophila brummer triglyceride Lipase.
title_full_unstemmed	Physiological and metabolomic consequences of reduced expression of the Drosophila brummer triglyceride Lipase.
title_sort	physiological and metabolomic consequences of reduced expression of the drosophila brummer triglyceride lipase.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/b451a74a50774c0fb3146510816e9adc
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Physiological and metabolomic consequences of reduced expression of the Drosophila brummer triglyceride Lipase.

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