Development of a sequencing system for spatial decoding of DNA barcode molecules at single-molecule resolution

Oguchi et al. developed an in-house sequencing system to spatially decode DNA barcode molecules at single-molecule resolution. They obtain an average read length of 20 nucleotides with an error rate of less than 5% per nucleotide. Leveraging this system, they devised a molecular foot printing method...

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Autores principales:	Yusuke Oguchi, Hirofumi Shintaku, Sotaro Uemura
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/b454774d269543398ffbe96da1e86973
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spelling	oai:doaj.org-article:b454774d269543398ffbe96da1e869732021-12-02T13:56:15ZDevelopment of a sequencing system for spatial decoding of DNA barcode molecules at single-molecule resolution10.1038/s42003-020-01499-82399-3642https://doaj.org/article/b454774d269543398ffbe96da1e869732020-12-01T00:00:00Zhttps://doi.org/10.1038/s42003-020-01499-8https://doaj.org/toc/2399-3642Oguchi et al. developed an in-house sequencing system to spatially decode DNA barcode molecules at single-molecule resolution. They obtain an average read length of 20 nucleotides with an error rate of less than 5% per nucleotide. Leveraging this system, they devised a molecular foot printing method that can be applied spatial proteomics as well as spatial transcriptomics.Yusuke OguchiHirofumi ShintakuSotaro UemuraNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 3, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Yusuke Oguchi Hirofumi Shintaku Sotaro Uemura Development of a sequencing system for spatial decoding of DNA barcode molecules at single-molecule resolution
description	Oguchi et al. developed an in-house sequencing system to spatially decode DNA barcode molecules at single-molecule resolution. They obtain an average read length of 20 nucleotides with an error rate of less than 5% per nucleotide. Leveraging this system, they devised a molecular foot printing method that can be applied spatial proteomics as well as spatial transcriptomics.
format	article
author	Yusuke Oguchi Hirofumi Shintaku Sotaro Uemura
author_facet	Yusuke Oguchi Hirofumi Shintaku Sotaro Uemura
author_sort	Yusuke Oguchi
title	Development of a sequencing system for spatial decoding of DNA barcode molecules at single-molecule resolution
title_short	Development of a sequencing system for spatial decoding of DNA barcode molecules at single-molecule resolution
title_full	Development of a sequencing system for spatial decoding of DNA barcode molecules at single-molecule resolution
title_fullStr	Development of a sequencing system for spatial decoding of DNA barcode molecules at single-molecule resolution
title_full_unstemmed	Development of a sequencing system for spatial decoding of DNA barcode molecules at single-molecule resolution
title_sort	development of a sequencing system for spatial decoding of dna barcode molecules at single-molecule resolution
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/b454774d269543398ffbe96da1e86973
work_keys_str_mv	AT yusukeoguchi developmentofasequencingsystemforspatialdecodingofdnabarcodemoleculesatsinglemoleculeresolution AT hirofumishintaku developmentofasequencingsystemforspatialdecodingofdnabarcodemoleculesatsinglemoleculeresolution AT sotarouemura developmentofasequencingsystemforspatialdecodingofdnabarcodemoleculesatsinglemoleculeresolution
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Development of a sequencing system for spatial decoding of DNA barcode molecules at single-molecule resolution

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