Detection of carbapenemase producing enterobacteria using an ion sensitive field effect transistor sensor

Abstract The timely and accurate detection of carbapenemase-producing Enterobacterales (CPE) is imperative to manage this worldwide problem in an effective fashion. Herein we addressed the question of whether the protons produced during imipenem hydrolysis could be detected using an ion sensitive fi...

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Autores principales:	Stathis D. Kotsakis, Georgios Miliotis, Eva Tzelepi, Leonidas S. Tzouvelekis, Vivi Miriagou
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b45705c467b049659fbc5b07312e3152
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spelling	oai:doaj.org-article:b45705c467b049659fbc5b07312e31522021-12-02T17:47:23ZDetection of carbapenemase producing enterobacteria using an ion sensitive field effect transistor sensor10.1038/s41598-021-91202-62045-2322https://doaj.org/article/b45705c467b049659fbc5b07312e31522021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91202-6https://doaj.org/toc/2045-2322Abstract The timely and accurate detection of carbapenemase-producing Enterobacterales (CPE) is imperative to manage this worldwide problem in an effective fashion. Herein we addressed the question of whether the protons produced during imipenem hydrolysis could be detected using an ion sensitive field effect transistor (ISFET). Application of the methodology on enzyme preparations showed that the sensor is able to detect carbapenemases of the NDM, IMP, KPC and NMC-A types at low nanomolar concentrations while VIM and OXA-48 responded at levels above 100 nM. Similar results were obtained when CPE cell suspensions were tested; NDM, IMP, NMC-A and KPC producers caused fast reductions of the output potential. Reduction rates with VIM-type and especially OXA-48 producing strains were significantly lower. Based on results with selected CPEs and carbapenemase-negative enterobacteria, a threshold of 10 mV drop at 30 min was set. Applying this threshold, the method exhibited 100% sensitivity for NDM, IMP and KPC and 77.3% for VIM producers. The OXA-48-positive strains failed to pass the detection threshold. A wide variety of carbapenemase-negative control strains were all classified as negative (100% specificity). In conclusion, an ISFET-based approach may have the potential to be routinely used for non OXA-48-like CPE detection in the clinical laboratory.Stathis D. KotsakisGeorgios MiliotisEva TzelepiLeonidas S. TzouvelekisVivi MiriagouNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stathis D. Kotsakis Georgios Miliotis Eva Tzelepi Leonidas S. Tzouvelekis Vivi Miriagou Detection of carbapenemase producing enterobacteria using an ion sensitive field effect transistor sensor
description	Abstract The timely and accurate detection of carbapenemase-producing Enterobacterales (CPE) is imperative to manage this worldwide problem in an effective fashion. Herein we addressed the question of whether the protons produced during imipenem hydrolysis could be detected using an ion sensitive field effect transistor (ISFET). Application of the methodology on enzyme preparations showed that the sensor is able to detect carbapenemases of the NDM, IMP, KPC and NMC-A types at low nanomolar concentrations while VIM and OXA-48 responded at levels above 100 nM. Similar results were obtained when CPE cell suspensions were tested; NDM, IMP, NMC-A and KPC producers caused fast reductions of the output potential. Reduction rates with VIM-type and especially OXA-48 producing strains were significantly lower. Based on results with selected CPEs and carbapenemase-negative enterobacteria, a threshold of 10 mV drop at 30 min was set. Applying this threshold, the method exhibited 100% sensitivity for NDM, IMP and KPC and 77.3% for VIM producers. The OXA-48-positive strains failed to pass the detection threshold. A wide variety of carbapenemase-negative control strains were all classified as negative (100% specificity). In conclusion, an ISFET-based approach may have the potential to be routinely used for non OXA-48-like CPE detection in the clinical laboratory.
format	article
author	Stathis D. Kotsakis Georgios Miliotis Eva Tzelepi Leonidas S. Tzouvelekis Vivi Miriagou
author_facet	Stathis D. Kotsakis Georgios Miliotis Eva Tzelepi Leonidas S. Tzouvelekis Vivi Miriagou
author_sort	Stathis D. Kotsakis
title	Detection of carbapenemase producing enterobacteria using an ion sensitive field effect transistor sensor
title_short	Detection of carbapenemase producing enterobacteria using an ion sensitive field effect transistor sensor
title_full	Detection of carbapenemase producing enterobacteria using an ion sensitive field effect transistor sensor
title_fullStr	Detection of carbapenemase producing enterobacteria using an ion sensitive field effect transistor sensor
title_full_unstemmed	Detection of carbapenemase producing enterobacteria using an ion sensitive field effect transistor sensor
title_sort	detection of carbapenemase producing enterobacteria using an ion sensitive field effect transistor sensor
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b45705c467b049659fbc5b07312e3152
work_keys_str_mv	AT stathisdkotsakis detectionofcarbapenemaseproducingenterobacteriausinganionsensitivefieldeffecttransistorsensor AT georgiosmiliotis detectionofcarbapenemaseproducingenterobacteriausinganionsensitivefieldeffecttransistorsensor AT evatzelepi detectionofcarbapenemaseproducingenterobacteriausinganionsensitivefieldeffecttransistorsensor AT leonidasstzouvelekis detectionofcarbapenemaseproducingenterobacteriausinganionsensitivefieldeffecttransistorsensor AT vivimiriagou detectionofcarbapenemaseproducingenterobacteriausinganionsensitivefieldeffecttransistorsensor
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Detection of carbapenemase producing enterobacteria using an ion sensitive field effect transistor sensor

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