Long non-coding RNA anti-differentiation non-coding RNA affects proliferation, invasion and migration of breast cancer cells by targeting miR-331

We aimed to evaluate the effects of long-chain non-coding RNA (lncRNA) anti-differentiation non-coding RNA (ANCR) on the proliferation, invasion and migration of breast cancer cells by targeting miR-331. Forty-eight breast cancer and paracancerous tissue samples were collected. LncRNA ANCR expressio...

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Autores principales:	Chaoyu Jiang, Xianbiao Shi, Dandan Yi, Ru Wang, Fazhan Xu, Wenxian Guan, Jianfeng Sang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	anti-differentiation non-coding rna breast cancer invasion migration mir-331 proliferation Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/b461e60c03394000b13ddee2f7cd4ce9
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spelling	oai:doaj.org-article:b461e60c03394000b13ddee2f7cd4ce92021-11-17T14:21:59ZLong non-coding RNA anti-differentiation non-coding RNA affects proliferation, invasion and migration of breast cancer cells by targeting miR-3312165-59792165-598710.1080/21655979.2021.2005989https://doaj.org/article/b461e60c03394000b13ddee2f7cd4ce92021-11-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2005989https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987We aimed to evaluate the effects of long-chain non-coding RNA (lncRNA) anti-differentiation non-coding RNA (ANCR) on the proliferation, invasion and migration of breast cancer cells by targeting miR-331. Forty-eight breast cancer and paracancerous tissue samples were collected. LncRNA ANCR expressions in breast cancer and adjacent tissues, human breast cancer cells and mammary epithelial cells, and miR-331 expressions in interfering cell line MDA-MB-231 (MCF-7)-shANCR, negative control MDA-MB-231 (MCF-7)-shNC and blank control MDA-MB-231 (MCF-7) were detected by real-time quantitative PCR. The correlations between LncRNA ANCR expression and clinicopathological characteristics were analyzed. Cell proliferation was detected by MTT and colony formation assays. Invasion and migration were tested by Transwell and scratch assays, respectively. The targeting relationship between ANCR and miR-331 was analyzed by TargetScan database, and their interaction was studied by dual luciferase reporter assay. The expression of lncRNA ANCR in breast cancer tissue was significantly lower than that in adjacent normal tissue (p<0.05). LncRNA ANCR was lowly expressed in various human breast cancer cell lines, being lowest in high-metastatic cell line (MDA-MB-231HM) (p<0.05). Silencing lncRNA ANCR significantly enhanced the proliferation and invasion capacities of breast cancer cells, and promoted their tumor formation abilities in nude mice (p<0.05). ANCR bound miR-331 targetedly, and the former negatively regulated the expression of the latter. LncRNA ANCR is lowly expressed upon breast cancer, and inhibits cell proliferation, invasion and migration in vitro and in vivo. LncRNA ANCR exerts antitumor effects by targetedly binding miR-331 and then inhibiting its expression.Chaoyu JiangXianbiao ShiDandan YiRu WangFazhan XuWenxian GuanJianfeng SangTaylor & Francis Grouparticleanti-differentiation non-coding rnabreast cancerinvasionmigrationmir-331proliferationBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	anti-differentiation non-coding rna breast cancer invasion migration mir-331 proliferation Biotechnology TP248.13-248.65
spellingShingle	anti-differentiation non-coding rna breast cancer invasion migration mir-331 proliferation Biotechnology TP248.13-248.65 Chaoyu Jiang Xianbiao Shi Dandan Yi Ru Wang Fazhan Xu Wenxian Guan Jianfeng Sang Long non-coding RNA anti-differentiation non-coding RNA affects proliferation, invasion and migration of breast cancer cells by targeting miR-331
description	We aimed to evaluate the effects of long-chain non-coding RNA (lncRNA) anti-differentiation non-coding RNA (ANCR) on the proliferation, invasion and migration of breast cancer cells by targeting miR-331. Forty-eight breast cancer and paracancerous tissue samples were collected. LncRNA ANCR expressions in breast cancer and adjacent tissues, human breast cancer cells and mammary epithelial cells, and miR-331 expressions in interfering cell line MDA-MB-231 (MCF-7)-shANCR, negative control MDA-MB-231 (MCF-7)-shNC and blank control MDA-MB-231 (MCF-7) were detected by real-time quantitative PCR. The correlations between LncRNA ANCR expression and clinicopathological characteristics were analyzed. Cell proliferation was detected by MTT and colony formation assays. Invasion and migration were tested by Transwell and scratch assays, respectively. The targeting relationship between ANCR and miR-331 was analyzed by TargetScan database, and their interaction was studied by dual luciferase reporter assay. The expression of lncRNA ANCR in breast cancer tissue was significantly lower than that in adjacent normal tissue (p<0.05). LncRNA ANCR was lowly expressed in various human breast cancer cell lines, being lowest in high-metastatic cell line (MDA-MB-231HM) (p<0.05). Silencing lncRNA ANCR significantly enhanced the proliferation and invasion capacities of breast cancer cells, and promoted their tumor formation abilities in nude mice (p<0.05). ANCR bound miR-331 targetedly, and the former negatively regulated the expression of the latter. LncRNA ANCR is lowly expressed upon breast cancer, and inhibits cell proliferation, invasion and migration in vitro and in vivo. LncRNA ANCR exerts antitumor effects by targetedly binding miR-331 and then inhibiting its expression.
format	article
author	Chaoyu Jiang Xianbiao Shi Dandan Yi Ru Wang Fazhan Xu Wenxian Guan Jianfeng Sang
author_facet	Chaoyu Jiang Xianbiao Shi Dandan Yi Ru Wang Fazhan Xu Wenxian Guan Jianfeng Sang
author_sort	Chaoyu Jiang
title	Long non-coding RNA anti-differentiation non-coding RNA affects proliferation, invasion and migration of breast cancer cells by targeting miR-331
title_short	Long non-coding RNA anti-differentiation non-coding RNA affects proliferation, invasion and migration of breast cancer cells by targeting miR-331
title_full	Long non-coding RNA anti-differentiation non-coding RNA affects proliferation, invasion and migration of breast cancer cells by targeting miR-331
title_fullStr	Long non-coding RNA anti-differentiation non-coding RNA affects proliferation, invasion and migration of breast cancer cells by targeting miR-331
title_full_unstemmed	Long non-coding RNA anti-differentiation non-coding RNA affects proliferation, invasion and migration of breast cancer cells by targeting miR-331
title_sort	long non-coding rna anti-differentiation non-coding rna affects proliferation, invasion and migration of breast cancer cells by targeting mir-331
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/b461e60c03394000b13ddee2f7cd4ce9
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Long non-coding RNA anti-differentiation non-coding RNA affects proliferation, invasion and migration of breast cancer cells by targeting miR-331

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