Long non-coding RNA anti-differentiation non-coding RNA affects proliferation, invasion and migration of breast cancer cells by targeting miR-331

We aimed to evaluate the effects of long-chain non-coding RNA (lncRNA) anti-differentiation non-coding RNA (ANCR) on the proliferation, invasion and migration of breast cancer cells by targeting miR-331. Forty-eight breast cancer and paracancerous tissue samples were collected. LncRNA ANCR expressio...

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Autores principales: Chaoyu Jiang, Xianbiao Shi, Dandan Yi, Ru Wang, Fazhan Xu, Wenxian Guan, Jianfeng Sang
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Lenguaje:EN
Publicado: Taylor & Francis Group 2021
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Acceso en línea:https://doaj.org/article/b461e60c03394000b13ddee2f7cd4ce9
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spelling oai:doaj.org-article:b461e60c03394000b13ddee2f7cd4ce92021-11-17T14:21:59ZLong non-coding RNA anti-differentiation non-coding RNA affects proliferation, invasion and migration of breast cancer cells by targeting miR-3312165-59792165-598710.1080/21655979.2021.2005989https://doaj.org/article/b461e60c03394000b13ddee2f7cd4ce92021-11-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2005989https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987We aimed to evaluate the effects of long-chain non-coding RNA (lncRNA) anti-differentiation non-coding RNA (ANCR) on the proliferation, invasion and migration of breast cancer cells by targeting miR-331. Forty-eight breast cancer and paracancerous tissue samples were collected. LncRNA ANCR expressions in breast cancer and adjacent tissues, human breast cancer cells and mammary epithelial cells, and miR-331 expressions in interfering cell line MDA-MB-231 (MCF-7)-shANCR, negative control MDA-MB-231 (MCF-7)-shNC and blank control MDA-MB-231 (MCF-7) were detected by real-time quantitative PCR. The correlations between LncRNA ANCR expression and clinicopathological characteristics were analyzed. Cell proliferation was detected by MTT and colony formation assays. Invasion and migration were tested by Transwell and scratch assays, respectively. The targeting relationship between ANCR and miR-331 was analyzed by TargetScan database, and their interaction was studied by dual luciferase reporter assay. The expression of lncRNA ANCR in breast cancer tissue was significantly lower than that in adjacent normal tissue (p<0.05). LncRNA ANCR was lowly expressed in various human breast cancer cell lines, being lowest in high-metastatic cell line (MDA-MB-231HM) (p<0.05). Silencing lncRNA ANCR significantly enhanced the proliferation and invasion capacities of breast cancer cells, and promoted their tumor formation abilities in nude mice (p<0.05). ANCR bound miR-331 targetedly, and the former negatively regulated the expression of the latter. LncRNA ANCR is lowly expressed upon breast cancer, and inhibits cell proliferation, invasion and migration in vitro and in vivo. LncRNA ANCR exerts antitumor effects by targetedly binding miR-331 and then inhibiting its expression.Chaoyu JiangXianbiao ShiDandan YiRu WangFazhan XuWenxian GuanJianfeng SangTaylor & Francis Grouparticleanti-differentiation non-coding rnabreast cancerinvasionmigrationmir-331proliferationBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution DOAJ
collection DOAJ
language EN
topic anti-differentiation non-coding rna
breast cancer
invasion
migration
mir-331
proliferation
Biotechnology
TP248.13-248.65
spellingShingle anti-differentiation non-coding rna
breast cancer
invasion
migration
mir-331
proliferation
Biotechnology
TP248.13-248.65
Chaoyu Jiang
Xianbiao Shi
Dandan Yi
Ru Wang
Fazhan Xu
Wenxian Guan
Jianfeng Sang
Long non-coding RNA anti-differentiation non-coding RNA affects proliferation, invasion and migration of breast cancer cells by targeting miR-331
description We aimed to evaluate the effects of long-chain non-coding RNA (lncRNA) anti-differentiation non-coding RNA (ANCR) on the proliferation, invasion and migration of breast cancer cells by targeting miR-331. Forty-eight breast cancer and paracancerous tissue samples were collected. LncRNA ANCR expressions in breast cancer and adjacent tissues, human breast cancer cells and mammary epithelial cells, and miR-331 expressions in interfering cell line MDA-MB-231 (MCF-7)-shANCR, negative control MDA-MB-231 (MCF-7)-shNC and blank control MDA-MB-231 (MCF-7) were detected by real-time quantitative PCR. The correlations between LncRNA ANCR expression and clinicopathological characteristics were analyzed. Cell proliferation was detected by MTT and colony formation assays. Invasion and migration were tested by Transwell and scratch assays, respectively. The targeting relationship between ANCR and miR-331 was analyzed by TargetScan database, and their interaction was studied by dual luciferase reporter assay. The expression of lncRNA ANCR in breast cancer tissue was significantly lower than that in adjacent normal tissue (p<0.05). LncRNA ANCR was lowly expressed in various human breast cancer cell lines, being lowest in high-metastatic cell line (MDA-MB-231HM) (p<0.05). Silencing lncRNA ANCR significantly enhanced the proliferation and invasion capacities of breast cancer cells, and promoted their tumor formation abilities in nude mice (p<0.05). ANCR bound miR-331 targetedly, and the former negatively regulated the expression of the latter. LncRNA ANCR is lowly expressed upon breast cancer, and inhibits cell proliferation, invasion and migration in vitro and in vivo. LncRNA ANCR exerts antitumor effects by targetedly binding miR-331 and then inhibiting its expression.
format article
author Chaoyu Jiang
Xianbiao Shi
Dandan Yi
Ru Wang
Fazhan Xu
Wenxian Guan
Jianfeng Sang
author_facet Chaoyu Jiang
Xianbiao Shi
Dandan Yi
Ru Wang
Fazhan Xu
Wenxian Guan
Jianfeng Sang
author_sort Chaoyu Jiang
title Long non-coding RNA anti-differentiation non-coding RNA affects proliferation, invasion and migration of breast cancer cells by targeting miR-331
title_short Long non-coding RNA anti-differentiation non-coding RNA affects proliferation, invasion and migration of breast cancer cells by targeting miR-331
title_full Long non-coding RNA anti-differentiation non-coding RNA affects proliferation, invasion and migration of breast cancer cells by targeting miR-331
title_fullStr Long non-coding RNA anti-differentiation non-coding RNA affects proliferation, invasion and migration of breast cancer cells by targeting miR-331
title_full_unstemmed Long non-coding RNA anti-differentiation non-coding RNA affects proliferation, invasion and migration of breast cancer cells by targeting miR-331
title_sort long non-coding rna anti-differentiation non-coding rna affects proliferation, invasion and migration of breast cancer cells by targeting mir-331
publisher Taylor & Francis Group
publishDate 2021
url https://doaj.org/article/b461e60c03394000b13ddee2f7cd4ce9
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