Endophytic bacteria producing antibacterial against methicillinresistant Staphylococcus aureus (MRSA) in seagrass from Rote Ndao, East Nusa Tenggara, Indonesia

Fitri DS, Pangastuti A, Susilowati A, Sutarno. 2017. Endophytic bacteria producing antibacterial against methicillin-resistant Staphylococcus aureus (MRSA) in seagrass from Rote Ndao, East Nusa Tenggara, Indonesia. Biodiversitas 18: 733-740. Methicillinresistant Staphylococcus aureus (MRSA) are bact...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	DIAN SAGITA FITRI, ARTINI PANGASTUTI, ARI SUSILOWATI, SUTARNO SUTARNO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2017
Materias:	antibacteria bacillus subtilis endophytic bacteria escherichia coli mrsa seagrass Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/b463f5bfe5bd4af4a3d1d7ae1d271718
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b463f5bfe5bd4af4a3d1d7ae1d271718
record_format	dspace
spelling	oai:doaj.org-article:b463f5bfe5bd4af4a3d1d7ae1d2717182021-11-16T13:52:30ZEndophytic bacteria producing antibacterial against methicillinresistant Staphylococcus aureus (MRSA) in seagrass from Rote Ndao, East Nusa Tenggara, Indonesia1412-033X2085-472210.13057/biodiv/d180244https://doaj.org/article/b463f5bfe5bd4af4a3d1d7ae1d2717182017-04-01T00:00:00Zhttps://smujo.id/biodiv/article/view/1703https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Fitri DS, Pangastuti A, Susilowati A, Sutarno. 2017. Endophytic bacteria producing antibacterial against methicillin-resistant Staphylococcus aureus (MRSA) in seagrass from Rote Ndao, East Nusa Tenggara, Indonesia. Biodiversitas 18: 733-740. Methicillinresistant Staphylococcus aureus (MRSA) are bacteria that resistant to the various type of antibiotics and yet cannot be handled comprehensively. The discovery of new antibiotic from endophytic bacteria in seagrass of Rote Island is an option to overcome the resistance. The aims of this research were to screen endophytic bacteria inhibit MRSA from seagrass, to determine the species of the endophytic bacteria and the genetic relationship. Isolation of endophytic bacteria has carried out by inoculating surface sterilization seagrass leaves on Marine Agar (MA) medium. Selection of potential endophytic bacteria-producing anti-MRSA has done using overlay method against MRSA, gram-positive Bacillus subtilis, and gram-negative Escherichia coli. Identification of the endophytic bacteria based on the sequence of 16S rRNA encoding gene. The results showed that there were eight isolates of endophytic bacteria which have antibacterial activity against MRSA of seagrass Enhalus acoroides, Thalassia hemprichii, and Cymodocea rotundata in the Litianak and Oeseli Beaches, Rote Ndao. The diameter of inhibition zone was between 0.65-18.27 mm with narrow spectrum with broad spectrum antibacterial activity. The eight potential endophytic bacteria identity were Bacillaceae E2M1, Bacillaceae E2M3, Bacillus E2M4, Bacillus E2M7, Bacillaceae E2M8, Pseudomonadaceae C1M7, Shewanellaceae C2M3, and Rhodobacteraceae T1M3. Most of the isolates can be said to be a new species as the percent similarity of 16S rRNA gene sequence was less than 95% and promising to produce new antibacterial compounds. Phylogenetic relationship showed some isolates clustering in different groups that present the diverse groups of endophytic bacteria were found.DIAN SAGITA FITRIARTINI PANGASTUTIARI SUSILOWATISUTARNO SUTARNOMBI & UNS Soloarticleantibacteriabacillus subtilisendophytic bacteriaescherichia colimrsaseagrassBiology (General)QH301-705.5ENBiodiversitas, Vol 18, Iss 2, Pp 733-740 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibacteria bacillus subtilis endophytic bacteria escherichia coli mrsa seagrass Biology (General) QH301-705.5
spellingShingle	antibacteria bacillus subtilis endophytic bacteria escherichia coli mrsa seagrass Biology (General) QH301-705.5 DIAN SAGITA FITRI ARTINI PANGASTUTI ARI SUSILOWATI SUTARNO SUTARNO Endophytic bacteria producing antibacterial against methicillinresistant Staphylococcus aureus (MRSA) in seagrass from Rote Ndao, East Nusa Tenggara, Indonesia
description	Fitri DS, Pangastuti A, Susilowati A, Sutarno. 2017. Endophytic bacteria producing antibacterial against methicillin-resistant Staphylococcus aureus (MRSA) in seagrass from Rote Ndao, East Nusa Tenggara, Indonesia. Biodiversitas 18: 733-740. Methicillinresistant Staphylococcus aureus (MRSA) are bacteria that resistant to the various type of antibiotics and yet cannot be handled comprehensively. The discovery of new antibiotic from endophytic bacteria in seagrass of Rote Island is an option to overcome the resistance. The aims of this research were to screen endophytic bacteria inhibit MRSA from seagrass, to determine the species of the endophytic bacteria and the genetic relationship. Isolation of endophytic bacteria has carried out by inoculating surface sterilization seagrass leaves on Marine Agar (MA) medium. Selection of potential endophytic bacteria-producing anti-MRSA has done using overlay method against MRSA, gram-positive Bacillus subtilis, and gram-negative Escherichia coli. Identification of the endophytic bacteria based on the sequence of 16S rRNA encoding gene. The results showed that there were eight isolates of endophytic bacteria which have antibacterial activity against MRSA of seagrass Enhalus acoroides, Thalassia hemprichii, and Cymodocea rotundata in the Litianak and Oeseli Beaches, Rote Ndao. The diameter of inhibition zone was between 0.65-18.27 mm with narrow spectrum with broad spectrum antibacterial activity. The eight potential endophytic bacteria identity were Bacillaceae E2M1, Bacillaceae E2M3, Bacillus E2M4, Bacillus E2M7, Bacillaceae E2M8, Pseudomonadaceae C1M7, Shewanellaceae C2M3, and Rhodobacteraceae T1M3. Most of the isolates can be said to be a new species as the percent similarity of 16S rRNA gene sequence was less than 95% and promising to produce new antibacterial compounds. Phylogenetic relationship showed some isolates clustering in different groups that present the diverse groups of endophytic bacteria were found.
format	article
author	DIAN SAGITA FITRI ARTINI PANGASTUTI ARI SUSILOWATI SUTARNO SUTARNO
author_facet	DIAN SAGITA FITRI ARTINI PANGASTUTI ARI SUSILOWATI SUTARNO SUTARNO
author_sort	DIAN SAGITA FITRI
title	Endophytic bacteria producing antibacterial against methicillinresistant Staphylococcus aureus (MRSA) in seagrass from Rote Ndao, East Nusa Tenggara, Indonesia
title_short	Endophytic bacteria producing antibacterial against methicillinresistant Staphylococcus aureus (MRSA) in seagrass from Rote Ndao, East Nusa Tenggara, Indonesia
title_full	Endophytic bacteria producing antibacterial against methicillinresistant Staphylococcus aureus (MRSA) in seagrass from Rote Ndao, East Nusa Tenggara, Indonesia
title_fullStr	Endophytic bacteria producing antibacterial against methicillinresistant Staphylococcus aureus (MRSA) in seagrass from Rote Ndao, East Nusa Tenggara, Indonesia
title_full_unstemmed	Endophytic bacteria producing antibacterial against methicillinresistant Staphylococcus aureus (MRSA) in seagrass from Rote Ndao, East Nusa Tenggara, Indonesia
title_sort	endophytic bacteria producing antibacterial against methicillinresistant staphylococcus aureus (mrsa) in seagrass from rote ndao, east nusa tenggara, indonesia
publisher	MBI & UNS Solo
publishDate	2017
url	https://doaj.org/article/b463f5bfe5bd4af4a3d1d7ae1d271718
work_keys_str_mv	AT diansagitafitri endophyticbacteriaproducingantibacterialagainstmethicillinresistantstaphylococcusaureusmrsainseagrassfromrotendaoeastnusatenggaraindonesia AT artinipangastuti endophyticbacteriaproducingantibacterialagainstmethicillinresistantstaphylococcusaureusmrsainseagrassfromrotendaoeastnusatenggaraindonesia AT arisusilowati endophyticbacteriaproducingantibacterialagainstmethicillinresistantstaphylococcusaureusmrsainseagrassfromrotendaoeastnusatenggaraindonesia AT sutarnosutarno endophyticbacteriaproducingantibacterialagainstmethicillinresistantstaphylococcusaureusmrsainseagrassfromrotendaoeastnusatenggaraindonesia
_version_	1718426500139581440

Endophytic bacteria producing antibacterial against methicillinresistant Staphylococcus aureus (MRSA) in seagrass from Rote Ndao, East Nusa Tenggara, Indonesia

Ejemplares similares