Differential synchrotron X-ray imaging markers based on the renal microvasculature for tubulointerstitial lesions and glomerulopathy

Abstract High resolution synchrotron microtomography capable of revealing microvessels in three dimensional (3D) establishes distinct imaging markers of mouse kidney disease strongly associated to renal tubulointerstitial (TI) lesions and glomerulopathy. Two complementary mouse models of chronic kid...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yu-Chuan Lin, Yeukuang Hwu, Guo-Shu Huang, Michael Hsiao, Tsung-Tse Lee, Shun-Min Yang, Ting-Kuo Lee, Nan-Yow Chen, Sung-Sen Yang, Ann Chen, Shuk-Man Ka
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b46495e4cc53411eb6c3b74623caa271
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b46495e4cc53411eb6c3b74623caa271
record_format	dspace
spelling	oai:doaj.org-article:b46495e4cc53411eb6c3b74623caa2712021-12-02T11:52:41ZDifferential synchrotron X-ray imaging markers based on the renal microvasculature for tubulointerstitial lesions and glomerulopathy10.1038/s41598-017-03677-x2045-2322https://doaj.org/article/b46495e4cc53411eb6c3b74623caa2712017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03677-xhttps://doaj.org/toc/2045-2322Abstract High resolution synchrotron microtomography capable of revealing microvessels in three dimensional (3D) establishes distinct imaging markers of mouse kidney disease strongly associated to renal tubulointerstitial (TI) lesions and glomerulopathy. Two complementary mouse models of chronic kidney disease (CKD), unilateral ureteral obstruction (UUO) and focal segmental glomerulosclerosis (FSGS), were used and five candidates of unique 3D imaging markers were identified. Our characterization to differentially reflect the altered microvasculature of renal TI lesions and/or glomerulopathy demonstrated these image features can be used to differentiate the disease status and the possible cause therefore qualified as image markers. These 3D imaging markers were further correlated with the histopathology and renal microvessel-based molecular study using antibodies against vascular endothelial cells (CD31), the connective tissue growth factor or the vascular endothelial growth factor. We also found that these 3D imaging markers individually characterize the development of renal TI lesions or glomerulopathy, quantitative and integrated use of all of them provide more information for differentiating the two renal conditions. Our findings thus establish a practical strategy to characterize the CKD-associated renal injuries by the microangiography-based 3D imaging and highlight the impact of dysfunctional microvasculature as a whole on the pathogenesis of the renal lesions.Yu-Chuan LinYeukuang HwuGuo-Shu HuangMichael HsiaoTsung-Tse LeeShun-Min YangTing-Kuo LeeNan-Yow ChenSung-Sen YangAnn ChenShuk-Man KaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yu-Chuan Lin Yeukuang Hwu Guo-Shu Huang Michael Hsiao Tsung-Tse Lee Shun-Min Yang Ting-Kuo Lee Nan-Yow Chen Sung-Sen Yang Ann Chen Shuk-Man Ka Differential synchrotron X-ray imaging markers based on the renal microvasculature for tubulointerstitial lesions and glomerulopathy
description	Abstract High resolution synchrotron microtomography capable of revealing microvessels in three dimensional (3D) establishes distinct imaging markers of mouse kidney disease strongly associated to renal tubulointerstitial (TI) lesions and glomerulopathy. Two complementary mouse models of chronic kidney disease (CKD), unilateral ureteral obstruction (UUO) and focal segmental glomerulosclerosis (FSGS), were used and five candidates of unique 3D imaging markers were identified. Our characterization to differentially reflect the altered microvasculature of renal TI lesions and/or glomerulopathy demonstrated these image features can be used to differentiate the disease status and the possible cause therefore qualified as image markers. These 3D imaging markers were further correlated with the histopathology and renal microvessel-based molecular study using antibodies against vascular endothelial cells (CD31), the connective tissue growth factor or the vascular endothelial growth factor. We also found that these 3D imaging markers individually characterize the development of renal TI lesions or glomerulopathy, quantitative and integrated use of all of them provide more information for differentiating the two renal conditions. Our findings thus establish a practical strategy to characterize the CKD-associated renal injuries by the microangiography-based 3D imaging and highlight the impact of dysfunctional microvasculature as a whole on the pathogenesis of the renal lesions.
format	article
author	Yu-Chuan Lin Yeukuang Hwu Guo-Shu Huang Michael Hsiao Tsung-Tse Lee Shun-Min Yang Ting-Kuo Lee Nan-Yow Chen Sung-Sen Yang Ann Chen Shuk-Man Ka
author_facet	Yu-Chuan Lin Yeukuang Hwu Guo-Shu Huang Michael Hsiao Tsung-Tse Lee Shun-Min Yang Ting-Kuo Lee Nan-Yow Chen Sung-Sen Yang Ann Chen Shuk-Man Ka
author_sort	Yu-Chuan Lin
title	Differential synchrotron X-ray imaging markers based on the renal microvasculature for tubulointerstitial lesions and glomerulopathy
title_short	Differential synchrotron X-ray imaging markers based on the renal microvasculature for tubulointerstitial lesions and glomerulopathy
title_full	Differential synchrotron X-ray imaging markers based on the renal microvasculature for tubulointerstitial lesions and glomerulopathy
title_fullStr	Differential synchrotron X-ray imaging markers based on the renal microvasculature for tubulointerstitial lesions and glomerulopathy
title_full_unstemmed	Differential synchrotron X-ray imaging markers based on the renal microvasculature for tubulointerstitial lesions and glomerulopathy
title_sort	differential synchrotron x-ray imaging markers based on the renal microvasculature for tubulointerstitial lesions and glomerulopathy
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b46495e4cc53411eb6c3b74623caa271
work_keys_str_mv	AT yuchuanlin differentialsynchrotronxrayimagingmarkersbasedontherenalmicrovasculaturefortubulointerstitiallesionsandglomerulopathy AT yeukuanghwu differentialsynchrotronxrayimagingmarkersbasedontherenalmicrovasculaturefortubulointerstitiallesionsandglomerulopathy AT guoshuhuang differentialsynchrotronxrayimagingmarkersbasedontherenalmicrovasculaturefortubulointerstitiallesionsandglomerulopathy AT michaelhsiao differentialsynchrotronxrayimagingmarkersbasedontherenalmicrovasculaturefortubulointerstitiallesionsandglomerulopathy AT tsungtselee differentialsynchrotronxrayimagingmarkersbasedontherenalmicrovasculaturefortubulointerstitiallesionsandglomerulopathy AT shunminyang differentialsynchrotronxrayimagingmarkersbasedontherenalmicrovasculaturefortubulointerstitiallesionsandglomerulopathy AT tingkuolee differentialsynchrotronxrayimagingmarkersbasedontherenalmicrovasculaturefortubulointerstitiallesionsandglomerulopathy AT nanyowchen differentialsynchrotronxrayimagingmarkersbasedontherenalmicrovasculaturefortubulointerstitiallesionsandglomerulopathy AT sungsenyang differentialsynchrotronxrayimagingmarkersbasedontherenalmicrovasculaturefortubulointerstitiallesionsandglomerulopathy AT annchen differentialsynchrotronxrayimagingmarkersbasedontherenalmicrovasculaturefortubulointerstitiallesionsandglomerulopathy AT shukmanka differentialsynchrotronxrayimagingmarkersbasedontherenalmicrovasculaturefortubulointerstitiallesionsandglomerulopathy
_version_	1718395006293639168

Differential synchrotron X-ray imaging markers based on the renal microvasculature for tubulointerstitial lesions and glomerulopathy

Ejemplares similares