Integrated NMR and cryo-EM atomic-resolution structure determination of a half-megadalton enzyme complex

NMR structure determination is challenging for proteins with a molecular weight above 30 kDa and atomic-resolution structure determination from cryo-EM data is currently not the rule. Here the authors describe an integrated structure determination approach that simultaneously uses NMR and EM data an...

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Autores principales:	Diego F. Gauto, Leandro F. Estrozi, Charles D. Schwieters, Gregory Effantin, Pavel Macek, Remy Sounier, Astrid C. Sivertsen, Elena Schmidt, Rime Kerfah, Guillaume Mas, Jacques-Philippe Colletier, Peter Güntert, Adrien Favier, Guy Schoehn, Paul Schanda, Jerome Boisbouvier
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/b46e113a51c845d98317d17073be2ebe
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spelling	oai:doaj.org-article:b46e113a51c845d98317d17073be2ebe2021-12-02T15:36:19ZIntegrated NMR and cryo-EM atomic-resolution structure determination of a half-megadalton enzyme complex10.1038/s41467-019-10490-92041-1723https://doaj.org/article/b46e113a51c845d98317d17073be2ebe2019-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-10490-9https://doaj.org/toc/2041-1723NMR structure determination is challenging for proteins with a molecular weight above 30 kDa and atomic-resolution structure determination from cryo-EM data is currently not the rule. Here the authors describe an integrated structure determination approach that simultaneously uses NMR and EM data and allows them to determine the structure of the 468 kDa dodecameric aminopeptidase TET2 complex.Diego F. GautoLeandro F. EstroziCharles D. SchwietersGregory EffantinPavel MacekRemy SounierAstrid C. SivertsenElena SchmidtRime KerfahGuillaume MasJacques-Philippe ColletierPeter GüntertAdrien FavierGuy SchoehnPaul SchandaJerome BoisbouvierNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-12 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Diego F. Gauto Leandro F. Estrozi Charles D. Schwieters Gregory Effantin Pavel Macek Remy Sounier Astrid C. Sivertsen Elena Schmidt Rime Kerfah Guillaume Mas Jacques-Philippe Colletier Peter Güntert Adrien Favier Guy Schoehn Paul Schanda Jerome Boisbouvier Integrated NMR and cryo-EM atomic-resolution structure determination of a half-megadalton enzyme complex
description	NMR structure determination is challenging for proteins with a molecular weight above 30 kDa and atomic-resolution structure determination from cryo-EM data is currently not the rule. Here the authors describe an integrated structure determination approach that simultaneously uses NMR and EM data and allows them to determine the structure of the 468 kDa dodecameric aminopeptidase TET2 complex.
format	article
author	Diego F. Gauto Leandro F. Estrozi Charles D. Schwieters Gregory Effantin Pavel Macek Remy Sounier Astrid C. Sivertsen Elena Schmidt Rime Kerfah Guillaume Mas Jacques-Philippe Colletier Peter Güntert Adrien Favier Guy Schoehn Paul Schanda Jerome Boisbouvier
author_facet	Diego F. Gauto Leandro F. Estrozi Charles D. Schwieters Gregory Effantin Pavel Macek Remy Sounier Astrid C. Sivertsen Elena Schmidt Rime Kerfah Guillaume Mas Jacques-Philippe Colletier Peter Güntert Adrien Favier Guy Schoehn Paul Schanda Jerome Boisbouvier
author_sort	Diego F. Gauto
title	Integrated NMR and cryo-EM atomic-resolution structure determination of a half-megadalton enzyme complex
title_short	Integrated NMR and cryo-EM atomic-resolution structure determination of a half-megadalton enzyme complex
title_full	Integrated NMR and cryo-EM atomic-resolution structure determination of a half-megadalton enzyme complex
title_fullStr	Integrated NMR and cryo-EM atomic-resolution structure determination of a half-megadalton enzyme complex
title_full_unstemmed	Integrated NMR and cryo-EM atomic-resolution structure determination of a half-megadalton enzyme complex
title_sort	integrated nmr and cryo-em atomic-resolution structure determination of a half-megadalton enzyme complex
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/b46e113a51c845d98317d17073be2ebe
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Integrated NMR and cryo-EM atomic-resolution structure determination of a half-megadalton enzyme complex

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