Comparative transcriptome analysis of cells from different areas reveals ROS responsive mechanism at sclerotial initiation stage in Morchella importuna

Abstract Morels are some of the most highly prized edible and medicinal mushrooms, with great economic and scientific value. Outdoor cultivation has been achieved and expanded on a large scale in China in recent years. Sclerotial formation is one of the most important phases during the morel life cy...

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Autores principales:	Qizheng Liu, Guoqiang He, Jinkang Wei, Caihong Dong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b471966b0d95491cad912bdbbed4569c
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spelling	oai:doaj.org-article:b471966b0d95491cad912bdbbed4569c2021-12-02T16:49:07ZComparative transcriptome analysis of cells from different areas reveals ROS responsive mechanism at sclerotial initiation stage in Morchella importuna10.1038/s41598-021-87784-w2045-2322https://doaj.org/article/b471966b0d95491cad912bdbbed4569c2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87784-whttps://doaj.org/toc/2045-2322Abstract Morels are some of the most highly prized edible and medicinal mushrooms, with great economic and scientific value. Outdoor cultivation has been achieved and expanded on a large scale in China in recent years. Sclerotial formation is one of the most important phases during the morel life cycle, and previous reports indicated that reactive oxygen species (ROS) play an important role. However, ROS response mechanisms at sclerotial initiation (SI) stage are poorly understood. In this study, comparative transcriptome analyses were performed with sclerotial and hyphal cells at different areas in the same plate at SI stage. Gene expression was significantly different at SI stage between sclerotial formation and mycelia growth areas. GO and KEGG analyses indicated more vigorous metabolic characteristics in the hyphae area, while transcription process, DNA repair, and protein processing were enriched in sclerotial cells. Gene expression related to H2O2 production was high in the hyphae area, while expression of H2O2-scavenging genes was high in sclerotial cells, leading to a higher H2O2 concentration in the hyphal region than in the sclerotium. Minor differences were observed in gene expression of H2O2-induced signaling pathway in sclerotial and hyphal cells; however, expression levels of the target genes of transcription factor MSN2, important in the H2O2-induced signaling pathways, were significantly different. MSN2 enhanced stress response regulation in sclerotia by regulating these target genes. Small molecular HSPs were also found upregulated in sclerotial cells. This study indicated that sclerotial cells are more resistant to ROS stress than hyphal cells through transcriptional regulation of related genes.Qizheng LiuGuoqiang HeJinkang WeiCaihong DongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qizheng Liu Guoqiang He Jinkang Wei Caihong Dong Comparative transcriptome analysis of cells from different areas reveals ROS responsive mechanism at sclerotial initiation stage in Morchella importuna
description	Abstract Morels are some of the most highly prized edible and medicinal mushrooms, with great economic and scientific value. Outdoor cultivation has been achieved and expanded on a large scale in China in recent years. Sclerotial formation is one of the most important phases during the morel life cycle, and previous reports indicated that reactive oxygen species (ROS) play an important role. However, ROS response mechanisms at sclerotial initiation (SI) stage are poorly understood. In this study, comparative transcriptome analyses were performed with sclerotial and hyphal cells at different areas in the same plate at SI stage. Gene expression was significantly different at SI stage between sclerotial formation and mycelia growth areas. GO and KEGG analyses indicated more vigorous metabolic characteristics in the hyphae area, while transcription process, DNA repair, and protein processing were enriched in sclerotial cells. Gene expression related to H2O2 production was high in the hyphae area, while expression of H2O2-scavenging genes was high in sclerotial cells, leading to a higher H2O2 concentration in the hyphal region than in the sclerotium. Minor differences were observed in gene expression of H2O2-induced signaling pathway in sclerotial and hyphal cells; however, expression levels of the target genes of transcription factor MSN2, important in the H2O2-induced signaling pathways, were significantly different. MSN2 enhanced stress response regulation in sclerotia by regulating these target genes. Small molecular HSPs were also found upregulated in sclerotial cells. This study indicated that sclerotial cells are more resistant to ROS stress than hyphal cells through transcriptional regulation of related genes.
format	article
author	Qizheng Liu Guoqiang He Jinkang Wei Caihong Dong
author_facet	Qizheng Liu Guoqiang He Jinkang Wei Caihong Dong
author_sort	Qizheng Liu
title	Comparative transcriptome analysis of cells from different areas reveals ROS responsive mechanism at sclerotial initiation stage in Morchella importuna
title_short	Comparative transcriptome analysis of cells from different areas reveals ROS responsive mechanism at sclerotial initiation stage in Morchella importuna
title_full	Comparative transcriptome analysis of cells from different areas reveals ROS responsive mechanism at sclerotial initiation stage in Morchella importuna
title_fullStr	Comparative transcriptome analysis of cells from different areas reveals ROS responsive mechanism at sclerotial initiation stage in Morchella importuna
title_full_unstemmed	Comparative transcriptome analysis of cells from different areas reveals ROS responsive mechanism at sclerotial initiation stage in Morchella importuna
title_sort	comparative transcriptome analysis of cells from different areas reveals ros responsive mechanism at sclerotial initiation stage in morchella importuna
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b471966b0d95491cad912bdbbed4569c
work_keys_str_mv	AT qizhengliu comparativetranscriptomeanalysisofcellsfromdifferentareasrevealsrosresponsivemechanismatsclerotialinitiationstageinmorchellaimportuna AT guoqianghe comparativetranscriptomeanalysisofcellsfromdifferentareasrevealsrosresponsivemechanismatsclerotialinitiationstageinmorchellaimportuna AT jinkangwei comparativetranscriptomeanalysisofcellsfromdifferentareasrevealsrosresponsivemechanismatsclerotialinitiationstageinmorchellaimportuna AT caihongdong comparativetranscriptomeanalysisofcellsfromdifferentareasrevealsrosresponsivemechanismatsclerotialinitiationstageinmorchellaimportuna
_version_	1718383416270913536

Comparative transcriptome analysis of cells from different areas reveals ROS responsive mechanism at sclerotial initiation stage in Morchella importuna

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