A fractal nature for polymerized laminin.

A fractal nature for polymerized laminin.

Polylaminin (polyLM) is a non-covalent acid-induced nano- and micro-structured polymer of the protein laminin displaying distinguished biological properties. Polylaminin stimulates neuritogenesis beyond the levels achieved by ordinary laminin and has been shown to promote axonal regeneration in anim...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Camila Hochman-Mendez, Marco Cantini, David Moratal, Manuel Salmeron-Sanchez, Tatiana Coelho-Sampaio
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2014
Materias:
Medicine
R
Science
Q
Acceso en línea:https://doaj.org/article/b4731583d9ba42b18149dfbdacade051
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:b4731583d9ba42b18149dfbdacade051
record_format dspace
spelling oai:doaj.org-article:b4731583d9ba42b18149dfbdacade0512021-11-25T05:57:26ZA fractal nature for polymerized laminin.1932-620310.1371/journal.pone.0109388https://doaj.org/article/b4731583d9ba42b18149dfbdacade0512014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0109388https://doaj.org/toc/1932-6203Polylaminin (polyLM) is a non-covalent acid-induced nano- and micro-structured polymer of the protein laminin displaying distinguished biological properties. Polylaminin stimulates neuritogenesis beyond the levels achieved by ordinary laminin and has been shown to promote axonal regeneration in animal models of spinal cord injury. Here we used confocal fluorescence microscopy (CFM), scanning electron microscopy (SEM) and atomic force microscopy (AFM) to characterize its three-dimensional structure. Renderization of confocal optical slices of immunostained polyLM revealed the aspect of a loose flocculated meshwork, which was homogeneously stained by the antibody. On the other hand, an ordinary matrix obtained upon adsorption of laminin in neutral pH (LM) was constituted of bulky protein aggregates whose interior was not accessible to the same anti-laminin antibody. SEM and AFM analyses revealed that the seed unit of polyLM was a flat polygon formed in solution whereas the seed structure of LM was highly heterogeneous, intercalating rod-like, spherical and thin spread lamellar deposits. As polyLM was visualized at progressively increasing magnifications, we observed that the morphology of the polymer was alike independently of the magnification used for the observation. A search for the Hausdorff dimension in images of the two matrices showed that polyLM, but not LM, presented fractal dimensions of 1.55, 1.62 and 1.70 after 1, 8 and 12 hours of adsorption, respectively. Data in the present work suggest that the intrinsic fractal nature of polymerized laminin can be the structural basis for the fractal-like organization of basement membranes in the neurogenic niches of the central nervous system.Camila Hochman-MendezMarco CantiniDavid MoratalManuel Salmeron-SanchezTatiana Coelho-SampaioPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e109388 (2014)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Camila Hochman-Mendez
Marco Cantini
David Moratal
Manuel Salmeron-Sanchez
Tatiana Coelho-Sampaio
A fractal nature for polymerized laminin.
description Polylaminin (polyLM) is a non-covalent acid-induced nano- and micro-structured polymer of the protein laminin displaying distinguished biological properties. Polylaminin stimulates neuritogenesis beyond the levels achieved by ordinary laminin and has been shown to promote axonal regeneration in animal models of spinal cord injury. Here we used confocal fluorescence microscopy (CFM), scanning electron microscopy (SEM) and atomic force microscopy (AFM) to characterize its three-dimensional structure. Renderization of confocal optical slices of immunostained polyLM revealed the aspect of a loose flocculated meshwork, which was homogeneously stained by the antibody. On the other hand, an ordinary matrix obtained upon adsorption of laminin in neutral pH (LM) was constituted of bulky protein aggregates whose interior was not accessible to the same anti-laminin antibody. SEM and AFM analyses revealed that the seed unit of polyLM was a flat polygon formed in solution whereas the seed structure of LM was highly heterogeneous, intercalating rod-like, spherical and thin spread lamellar deposits. As polyLM was visualized at progressively increasing magnifications, we observed that the morphology of the polymer was alike independently of the magnification used for the observation. A search for the Hausdorff dimension in images of the two matrices showed that polyLM, but not LM, presented fractal dimensions of 1.55, 1.62 and 1.70 after 1, 8 and 12 hours of adsorption, respectively. Data in the present work suggest that the intrinsic fractal nature of polymerized laminin can be the structural basis for the fractal-like organization of basement membranes in the neurogenic niches of the central nervous system.
format article
author Camila Hochman-Mendez
Marco Cantini
David Moratal
Manuel Salmeron-Sanchez
Tatiana Coelho-Sampaio
author_facet Camila Hochman-Mendez
Marco Cantini
David Moratal
Manuel Salmeron-Sanchez
Tatiana Coelho-Sampaio
author_sort Camila Hochman-Mendez
title A fractal nature for polymerized laminin.
title_short A fractal nature for polymerized laminin.
title_full A fractal nature for polymerized laminin.
title_fullStr A fractal nature for polymerized laminin.
title_full_unstemmed A fractal nature for polymerized laminin.
title_sort fractal nature for polymerized laminin.
publisher Public Library of Science (PLoS)
publishDate 2014
url https://doaj.org/article/b4731583d9ba42b18149dfbdacade051
work_keys_str_mv AT camilahochmanmendez afractalnatureforpolymerizedlaminin
AT marcocantini afractalnatureforpolymerizedlaminin
AT davidmoratal afractalnatureforpolymerizedlaminin
AT manuelsalmeronsanchez afractalnatureforpolymerizedlaminin
AT tatianacoelhosampaio afractalnatureforpolymerizedlaminin
AT camilahochmanmendez fractalnatureforpolymerizedlaminin
AT marcocantini fractalnatureforpolymerizedlaminin
AT davidmoratal fractalnatureforpolymerizedlaminin
AT manuelsalmeronsanchez fractalnatureforpolymerizedlaminin
AT tatianacoelhosampaio fractalnatureforpolymerizedlaminin
_version_ 1718414362030374912

Ejemplares similares