Human endometrial CD98 is essential for blastocyst adhesion.

<h4>Background</h4>Understanding the molecular basis of embryonic implantation is of great clinical and biological relevance. Little is currently known about the adhesion receptors that determine endometrial receptivity for embryonic implantation in humans.<h4>Methods and principal...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Francisco Domínguez, Carlos Simón, Alicia Quiñonero, Miguel Ángel Ramírez, Elena González-Muñoz, Hans Burghardt, Ana Cervero, Sebastián Martínez, Antonio Pellicer, Manuel Palacín, Francisco Sánchez-Madrid, María Yáñez-Mó
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b473190798e84c9183c6ecbb1fd4ee27
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b473190798e84c9183c6ecbb1fd4ee27
record_format	dspace
spelling	oai:doaj.org-article:b473190798e84c9183c6ecbb1fd4ee272021-11-18T07:03:16ZHuman endometrial CD98 is essential for blastocyst adhesion.1932-620310.1371/journal.pone.0013380https://doaj.org/article/b473190798e84c9183c6ecbb1fd4ee272010-10-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20976164/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Understanding the molecular basis of embryonic implantation is of great clinical and biological relevance. Little is currently known about the adhesion receptors that determine endometrial receptivity for embryonic implantation in humans.<h4>Methods and principal findings</h4>Using two human endometrial cell lines characterized by low and high receptivity, we identified the membrane receptor CD98 as a novel molecule selectively and significantly associated with the receptive phenotype. In human endometrial samples, CD98 was the only molecule studied whose expression was restricted to the implantation window in human endometrial tissue. CD98 expression was restricted to the apical surface and included in tetraspanin-enriched microdomains of primary endometrial epithelial cells, as demonstrated by the biochemical association between CD98 and tetraspanin CD9. CD98 expression was induced in vitro by treatment of primary endometrial epithelial cells with human chorionic gonadotropin, 17-β-estradiol, LIF or EGF. Endometrial overexpression of CD98 or tetraspanin CD9 greatly enhanced mouse blastocyst adhesion, while their siRNA-mediated depletion reduced the blastocyst adhesion rate.<h4>Conclusions</h4>These results indicate that CD98, a component of tetraspanin-enriched microdomains, appears to be an important determinant of human endometrial receptivity during the implantation window.Francisco DomínguezCarlos SimónAlicia QuiñoneroMiguel Ángel RamírezElena González-MuñozHans BurghardtAna CerveroSebastián MartínezAntonio PellicerManuel PalacínFrancisco Sánchez-MadridMaría Yáñez-MóPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 10, p e13380 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Francisco Domínguez Carlos Simón Alicia Quiñonero Miguel Ángel Ramírez Elena González-Muñoz Hans Burghardt Ana Cervero Sebastián Martínez Antonio Pellicer Manuel Palacín Francisco Sánchez-Madrid María Yáñez-Mó Human endometrial CD98 is essential for blastocyst adhesion.
description	<h4>Background</h4>Understanding the molecular basis of embryonic implantation is of great clinical and biological relevance. Little is currently known about the adhesion receptors that determine endometrial receptivity for embryonic implantation in humans.<h4>Methods and principal findings</h4>Using two human endometrial cell lines characterized by low and high receptivity, we identified the membrane receptor CD98 as a novel molecule selectively and significantly associated with the receptive phenotype. In human endometrial samples, CD98 was the only molecule studied whose expression was restricted to the implantation window in human endometrial tissue. CD98 expression was restricted to the apical surface and included in tetraspanin-enriched microdomains of primary endometrial epithelial cells, as demonstrated by the biochemical association between CD98 and tetraspanin CD9. CD98 expression was induced in vitro by treatment of primary endometrial epithelial cells with human chorionic gonadotropin, 17-β-estradiol, LIF or EGF. Endometrial overexpression of CD98 or tetraspanin CD9 greatly enhanced mouse blastocyst adhesion, while their siRNA-mediated depletion reduced the blastocyst adhesion rate.<h4>Conclusions</h4>These results indicate that CD98, a component of tetraspanin-enriched microdomains, appears to be an important determinant of human endometrial receptivity during the implantation window.
format	article
author	Francisco Domínguez Carlos Simón Alicia Quiñonero Miguel Ángel Ramírez Elena González-Muñoz Hans Burghardt Ana Cervero Sebastián Martínez Antonio Pellicer Manuel Palacín Francisco Sánchez-Madrid María Yáñez-Mó
author_facet	Francisco Domínguez Carlos Simón Alicia Quiñonero Miguel Ángel Ramírez Elena González-Muñoz Hans Burghardt Ana Cervero Sebastián Martínez Antonio Pellicer Manuel Palacín Francisco Sánchez-Madrid María Yáñez-Mó
author_sort	Francisco Domínguez
title	Human endometrial CD98 is essential for blastocyst adhesion.
title_short	Human endometrial CD98 is essential for blastocyst adhesion.
title_full	Human endometrial CD98 is essential for blastocyst adhesion.
title_fullStr	Human endometrial CD98 is essential for blastocyst adhesion.
title_full_unstemmed	Human endometrial CD98 is essential for blastocyst adhesion.
title_sort	human endometrial cd98 is essential for blastocyst adhesion.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/b473190798e84c9183c6ecbb1fd4ee27
work_keys_str_mv	AT franciscodominguez humanendometrialcd98isessentialforblastocystadhesion AT carlossimon humanendometrialcd98isessentialforblastocystadhesion AT aliciaquinonero humanendometrialcd98isessentialforblastocystadhesion AT miguelangelramirez humanendometrialcd98isessentialforblastocystadhesion AT elenagonzalezmunoz humanendometrialcd98isessentialforblastocystadhesion AT hansburghardt humanendometrialcd98isessentialforblastocystadhesion AT anacervero humanendometrialcd98isessentialforblastocystadhesion AT sebastianmartinez humanendometrialcd98isessentialforblastocystadhesion AT antoniopellicer humanendometrialcd98isessentialforblastocystadhesion AT manuelpalacin humanendometrialcd98isessentialforblastocystadhesion AT franciscosanchezmadrid humanendometrialcd98isessentialforblastocystadhesion AT mariayanezmo humanendometrialcd98isessentialforblastocystadhesion
_version_	1718424001482588160

Human endometrial CD98 is essential for blastocyst adhesion.

Ejemplares similares