Physical organization of DNA by multiple non-specific DNA-binding modes of integration host factor (IHF).

The integration host factor (IHF) is an abundant nucleoid-associated protein and an essential co-factor for phage λ site-specific recombination and gene regulation in E. coli. Introduction of a sharp DNA kink at specific cognate sites is critical for these functions. Interestingly, the intracellular...

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Autores principales:	Jie Lin, Hu Chen, Peter Dröge, Jie Yan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b486911a166e4210bf9ca06143042904
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spelling	oai:doaj.org-article:b486911a166e4210bf9ca061430429042021-11-18T08:08:39ZPhysical organization of DNA by multiple non-specific DNA-binding modes of integration host factor (IHF).1932-620310.1371/journal.pone.0049885https://doaj.org/article/b486911a166e4210bf9ca061430429042012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23166787/?tool=EBIhttps://doaj.org/toc/1932-6203The integration host factor (IHF) is an abundant nucleoid-associated protein and an essential co-factor for phage λ site-specific recombination and gene regulation in E. coli. Introduction of a sharp DNA kink at specific cognate sites is critical for these functions. Interestingly, the intracellular concentration of IHF is much higher than the concentration needed for site-specific interactions, suggesting that non-specific binding of IHF to DNA plays a role in the physical organization of bacterial chromatin. However, it is unclear how non-specific DNA association contributes to DNA organization. By using a combination of single DNA manipulation and atomic force microscopy imaging methods, we show here that distinct modes of non-specific DNA binding of IHF result in complex global DNA conformations. Changes in KCl and IHF concentrations, as well as tension applied to DNA, dramatically influence the degree of DNA-bending. In addition, IHF can crosslink DNA into a highly compact DNA meshwork that is observed in the presence of magnesium at low concentration of monovalent ions and high IHF-DNA stoichiometries. Our findings provide important insights into how IHF contributes to bacterial chromatin organization, gene regulation, and biofilm formation.Jie LinHu ChenPeter DrögeJie YanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e49885 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jie Lin Hu Chen Peter Dröge Jie Yan Physical organization of DNA by multiple non-specific DNA-binding modes of integration host factor (IHF).
description	The integration host factor (IHF) is an abundant nucleoid-associated protein and an essential co-factor for phage λ site-specific recombination and gene regulation in E. coli. Introduction of a sharp DNA kink at specific cognate sites is critical for these functions. Interestingly, the intracellular concentration of IHF is much higher than the concentration needed for site-specific interactions, suggesting that non-specific binding of IHF to DNA plays a role in the physical organization of bacterial chromatin. However, it is unclear how non-specific DNA association contributes to DNA organization. By using a combination of single DNA manipulation and atomic force microscopy imaging methods, we show here that distinct modes of non-specific DNA binding of IHF result in complex global DNA conformations. Changes in KCl and IHF concentrations, as well as tension applied to DNA, dramatically influence the degree of DNA-bending. In addition, IHF can crosslink DNA into a highly compact DNA meshwork that is observed in the presence of magnesium at low concentration of monovalent ions and high IHF-DNA stoichiometries. Our findings provide important insights into how IHF contributes to bacterial chromatin organization, gene regulation, and biofilm formation.
format	article
author	Jie Lin Hu Chen Peter Dröge Jie Yan
author_facet	Jie Lin Hu Chen Peter Dröge Jie Yan
author_sort	Jie Lin
title	Physical organization of DNA by multiple non-specific DNA-binding modes of integration host factor (IHF).
title_short	Physical organization of DNA by multiple non-specific DNA-binding modes of integration host factor (IHF).
title_full	Physical organization of DNA by multiple non-specific DNA-binding modes of integration host factor (IHF).
title_fullStr	Physical organization of DNA by multiple non-specific DNA-binding modes of integration host factor (IHF).
title_full_unstemmed	Physical organization of DNA by multiple non-specific DNA-binding modes of integration host factor (IHF).
title_sort	physical organization of dna by multiple non-specific dna-binding modes of integration host factor (ihf).
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/b486911a166e4210bf9ca06143042904
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Physical organization of DNA by multiple non-specific DNA-binding modes of integration host factor (IHF).

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