Comparative Analysis of Blood‐Derived Endothelial Cells for Designing Next‐Generation Personalized Organ‐on‐Chips

Background Organ‐on‐chip technology has accelerated in vitro preclinical research of the vascular system, and a key strength of this platform is its promise to impact personalized medicine by providing a primary human cell–culture environment where endothelial cells are directly biopsied from indivi...

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Autores principales:	Tanmay Mathur, James J. Tronolone, Abhishek Jain
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	blood outgrowth endothelial cells experimental cell model organ‐on‐chip vascular bioengineering Diseases of the circulatory (Cardiovascular) system RC666-701
Acceso en línea:	https://doaj.org/article/b48b8829c2a748bab648457c1bd3b761
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spelling	oai:doaj.org-article:b48b8829c2a748bab648457c1bd3b7612021-11-16T10:22:43ZComparative Analysis of Blood‐Derived Endothelial Cells for Designing Next‐Generation Personalized Organ‐on‐Chips10.1161/JAHA.121.0227952047-9980https://doaj.org/article/b48b8829c2a748bab648457c1bd3b7612021-11-01T00:00:00Zhttps://www.ahajournals.org/doi/10.1161/JAHA.121.022795https://doaj.org/toc/2047-9980Background Organ‐on‐chip technology has accelerated in vitro preclinical research of the vascular system, and a key strength of this platform is its promise to impact personalized medicine by providing a primary human cell–culture environment where endothelial cells are directly biopsied from individual tissue or differentiated through stem cell biotechniques. However, these methods are difficult to adopt in laboratories, and often result in impurity and heterogeneity of cells. This limits the power of organ‐chips in making accurate physiological predictions. In this study, we report the use of blood‐derived endothelial cells as alternatives to primary and induced pluripotent stem cell–derived endothelial cells. Methods and Results Here, the genotype, phenotype, and organ‐chip functional characteristics of blood‐derived outgrowth endothelial cells were compared against commercially available and most used primary endothelial cells and induced pluripotent stem cell–derived endothelial cells. The methods include RNA‐sequencing, as well as criterion standard assays of cell marker expression, growth kinetics, migration potential, and vasculogenesis. Finally, thromboinflammatory responses under shear using vessel‐chips engineered with blood‐derived endothelial cells were assessed. Blood‐derived endothelial cells exhibit the criterion standard hallmarks of typical endothelial cells. There are differences in gene expression profiles between different sources of endothelial cells, but blood‐derived cells are relatively closer to primary cells than induced pluripotent stem cell–derived. Furthermore, blood‐derived endothelial cells are much easier to obtain from individuals and yet, they serve as an equally effective cell source for functional studies and organ‐chips compared with primary cells or induced pluripotent stem cell–derived cells. Conclusions Blood‐derived endothelial cells may be used in preclinical research for developing more robust and personalized next‐generation disease models using organ‐on‐chips.Tanmay MathurJames J. TronoloneAbhishek JainWileyarticleblood outgrowth endothelial cellsexperimental cell modelorgan‐on‐chipvascular bioengineeringDiseases of the circulatory (Cardiovascular) systemRC666-701ENJournal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, Vol 10, Iss 22 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	blood outgrowth endothelial cells experimental cell model organ‐on‐chip vascular bioengineering Diseases of the circulatory (Cardiovascular) system RC666-701
spellingShingle	blood outgrowth endothelial cells experimental cell model organ‐on‐chip vascular bioengineering Diseases of the circulatory (Cardiovascular) system RC666-701 Tanmay Mathur James J. Tronolone Abhishek Jain Comparative Analysis of Blood‐Derived Endothelial Cells for Designing Next‐Generation Personalized Organ‐on‐Chips
description	Background Organ‐on‐chip technology has accelerated in vitro preclinical research of the vascular system, and a key strength of this platform is its promise to impact personalized medicine by providing a primary human cell–culture environment where endothelial cells are directly biopsied from individual tissue or differentiated through stem cell biotechniques. However, these methods are difficult to adopt in laboratories, and often result in impurity and heterogeneity of cells. This limits the power of organ‐chips in making accurate physiological predictions. In this study, we report the use of blood‐derived endothelial cells as alternatives to primary and induced pluripotent stem cell–derived endothelial cells. Methods and Results Here, the genotype, phenotype, and organ‐chip functional characteristics of blood‐derived outgrowth endothelial cells were compared against commercially available and most used primary endothelial cells and induced pluripotent stem cell–derived endothelial cells. The methods include RNA‐sequencing, as well as criterion standard assays of cell marker expression, growth kinetics, migration potential, and vasculogenesis. Finally, thromboinflammatory responses under shear using vessel‐chips engineered with blood‐derived endothelial cells were assessed. Blood‐derived endothelial cells exhibit the criterion standard hallmarks of typical endothelial cells. There are differences in gene expression profiles between different sources of endothelial cells, but blood‐derived cells are relatively closer to primary cells than induced pluripotent stem cell–derived. Furthermore, blood‐derived endothelial cells are much easier to obtain from individuals and yet, they serve as an equally effective cell source for functional studies and organ‐chips compared with primary cells or induced pluripotent stem cell–derived cells. Conclusions Blood‐derived endothelial cells may be used in preclinical research for developing more robust and personalized next‐generation disease models using organ‐on‐chips.
format	article
author	Tanmay Mathur James J. Tronolone Abhishek Jain
author_facet	Tanmay Mathur James J. Tronolone Abhishek Jain
author_sort	Tanmay Mathur
title	Comparative Analysis of Blood‐Derived Endothelial Cells for Designing Next‐Generation Personalized Organ‐on‐Chips
title_short	Comparative Analysis of Blood‐Derived Endothelial Cells for Designing Next‐Generation Personalized Organ‐on‐Chips
title_full	Comparative Analysis of Blood‐Derived Endothelial Cells for Designing Next‐Generation Personalized Organ‐on‐Chips
title_fullStr	Comparative Analysis of Blood‐Derived Endothelial Cells for Designing Next‐Generation Personalized Organ‐on‐Chips
title_full_unstemmed	Comparative Analysis of Blood‐Derived Endothelial Cells for Designing Next‐Generation Personalized Organ‐on‐Chips
title_sort	comparative analysis of blood‐derived endothelial cells for designing next‐generation personalized organ‐on‐chips
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/b48b8829c2a748bab648457c1bd3b761
work_keys_str_mv	AT tanmaymathur comparativeanalysisofbloodderivedendothelialcellsfordesigningnextgenerationpersonalizedorganonchips AT jamesjtronolone comparativeanalysisofbloodderivedendothelialcellsfordesigningnextgenerationpersonalizedorganonchips AT abhishekjain comparativeanalysisofbloodderivedendothelialcellsfordesigningnextgenerationpersonalizedorganonchips
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Comparative Analysis of Blood‐Derived Endothelial Cells for Designing Next‐Generation Personalized Organ‐on‐Chips

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