An efficient method for in vitro gene delivery via regulation of cellular endocytosis pathway

Jing Luo,1,2,* Caixia Li,3,* Jianlin Chen,1,2 Gang Wang,2 Rong Gao,1 Zhongwei Gu2 1Key Laboratory for Bio-Resource and Eco-Environment of Ministry of Education, Key Laboratory for Animal Disease Prevention and Food Safety of Sichuan Province, College of Life Science, Sichuan University, C...

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Autores principales:	Luo J, Li C, Chen J, Wang G, Gao R, Gu Z
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b493516d16f143cd9c7e16fdccf42d54
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spelling	oai:doaj.org-article:b493516d16f143cd9c7e16fdccf42d542021-12-02T05:04:30ZAn efficient method for in vitro gene delivery via regulation of cellular endocytosis pathway1178-2013https://doaj.org/article/b493516d16f143cd9c7e16fdccf42d542015-03-01T00:00:00Zhttp://www.dovepress.com/an-efficient-method-for-in-vitro-gene-delivery-via-regulation-of-cellu-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Jing Luo,1,2,* Caixia Li,3,* Jianlin Chen,1,2 Gang Wang,2 Rong Gao,1 Zhongwei Gu2 1Key Laboratory for Bio-Resource and Eco-Environment of Ministry of Education, Key Laboratory for Animal Disease Prevention and Food Safety of Sichuan Province, College of Life Science, Sichuan University, Chengdu, People’s Republic of China; 2National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, People’s Republic of China; 3Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, People’s Republic of China *These authors contributed equally to this work Abstract: Transfection efficiency was the primary goal for in vitro gene delivery mediated by nonviral gene carriers. Here, we report a modified gene transfection method that could greatly increase the efficiency of, and accelerate the process mediated by, 25 kDa branched polyethyleneimine and Lipofectamine™ 2000 in a broad range of cell strains, including tumor, normal, primary, and embryonic stem cells. In this method, the combination of transfection procedure with optimized complexation volume had a determinant effect on gene delivery result. The superiorities of the method were found to be related to the change of cellular endocytosis pathway and decrease of particle size. The efficient and simple method established in this study can be widely used for in vitro gene delivery into cultured cells. We think it may also be applicable for many more nonviral gene delivery materials than polyethyleneimine and liposome. Keywords: gene delivery, gene expression, endocytosis, polyethyleneimine, Lipofectamine™ 2000Luo JLi CChen JWang GGao RGu ZDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 1667-1678 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Luo J Li C Chen J Wang G Gao R Gu Z An efficient method for in vitro gene delivery via regulation of cellular endocytosis pathway
description	Jing Luo,1,2,* Caixia Li,3,* Jianlin Chen,1,2 Gang Wang,2 Rong Gao,1 Zhongwei Gu2 1Key Laboratory for Bio-Resource and Eco-Environment of Ministry of Education, Key Laboratory for Animal Disease Prevention and Food Safety of Sichuan Province, College of Life Science, Sichuan University, Chengdu, People’s Republic of China; 2National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, People’s Republic of China; 3Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, People’s Republic of China *These authors contributed equally to this work Abstract: Transfection efficiency was the primary goal for in vitro gene delivery mediated by nonviral gene carriers. Here, we report a modified gene transfection method that could greatly increase the efficiency of, and accelerate the process mediated by, 25 kDa branched polyethyleneimine and Lipofectamine™ 2000 in a broad range of cell strains, including tumor, normal, primary, and embryonic stem cells. In this method, the combination of transfection procedure with optimized complexation volume had a determinant effect on gene delivery result. The superiorities of the method were found to be related to the change of cellular endocytosis pathway and decrease of particle size. The efficient and simple method established in this study can be widely used for in vitro gene delivery into cultured cells. We think it may also be applicable for many more nonviral gene delivery materials than polyethyleneimine and liposome. Keywords: gene delivery, gene expression, endocytosis, polyethyleneimine, Lipofectamine™ 2000
format	article
author	Luo J Li C Chen J Wang G Gao R Gu Z
author_facet	Luo J Li C Chen J Wang G Gao R Gu Z
author_sort	Luo J
title	An efficient method for in vitro gene delivery via regulation of cellular endocytosis pathway
title_short	An efficient method for in vitro gene delivery via regulation of cellular endocytosis pathway
title_full	An efficient method for in vitro gene delivery via regulation of cellular endocytosis pathway
title_fullStr	An efficient method for in vitro gene delivery via regulation of cellular endocytosis pathway
title_full_unstemmed	An efficient method for in vitro gene delivery via regulation of cellular endocytosis pathway
title_sort	efficient method for in vitro gene delivery via regulation of cellular endocytosis pathway
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/b493516d16f143cd9c7e16fdccf42d54
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An efficient method for in vitro gene delivery via regulation of cellular endocytosis pathway

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