Synergistic Use of Hypocotyl Explants and High Bap Preconditioning for Enhanced Transformation Frequency in Brinjal (Solanum melongena L.)

Poor regeneration is one of the limiting factors in the development of transgenic crops since Agrobacterium as a plant pathogen can disturb the fragile in vitro conditions with wounding and infection regimes. We have tried to optimize the transformation system in two important varieties of brinjal a...

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Autores principales: Vageeshbabu S Hanur, D P Prakash, B S Deepali, R Asokan, Y L Ramachandra, Riaz Mahmood, Lalitha Anand
Formato: article
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Publicado: Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2006
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Acceso en línea:https://doaj.org/article/b49cfc24cd7e43539ed13648ae36c40b
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spelling oai:doaj.org-article:b49cfc24cd7e43539ed13648ae36c40b2021-12-02T12:52:11ZSynergistic Use of Hypocotyl Explants and High Bap Preconditioning for Enhanced Transformation Frequency in Brinjal (Solanum melongena L.)0973-354X2582-4899https://doaj.org/article/b49cfc24cd7e43539ed13648ae36c40b2006-12-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/650https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899Poor regeneration is one of the limiting factors in the development of transgenic crops since Agrobacterium as a plant pathogen can disturb the fragile in vitro conditions with wounding and infection regimes. We have tried to optimize the transformation system in two important varieties of brinjal after Agrobacterium infection to the explants. The effect of explant was studied and hypocotyls were found to be better than cotyledonary leaves. High BAP during the preconditioning period was found to further enhance the regeneration rate. Therefore, use of hypocotyls and high BAP during preconditioning can improve the regeneration of transformed cells and recovery of transformants in vegetables especially brinjal.Vageeshbabu S HanurD P PrakashB S DeepaliR AsokanY L RamachandraRiaz MahmoodLalitha AnandSociety for Promotion of Horticulture - Indian Institute of Horticultural Researcharticlesolanum melongenatransformationhypocotylbappreconditioningPlant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 1, Iss 2, Pp 116-119 (2006)
institution DOAJ
collection DOAJ
language EN
topic solanum melongena
transformation
hypocotyl
bap
preconditioning
Plant culture
SB1-1110
spellingShingle solanum melongena
transformation
hypocotyl
bap
preconditioning
Plant culture
SB1-1110
Vageeshbabu S Hanur
D P Prakash
B S Deepali
R Asokan
Y L Ramachandra
Riaz Mahmood
Lalitha Anand
Synergistic Use of Hypocotyl Explants and High Bap Preconditioning for Enhanced Transformation Frequency in Brinjal (Solanum melongena L.)
description Poor regeneration is one of the limiting factors in the development of transgenic crops since Agrobacterium as a plant pathogen can disturb the fragile in vitro conditions with wounding and infection regimes. We have tried to optimize the transformation system in two important varieties of brinjal after Agrobacterium infection to the explants. The effect of explant was studied and hypocotyls were found to be better than cotyledonary leaves. High BAP during the preconditioning period was found to further enhance the regeneration rate. Therefore, use of hypocotyls and high BAP during preconditioning can improve the regeneration of transformed cells and recovery of transformants in vegetables especially brinjal.
format article
author Vageeshbabu S Hanur
D P Prakash
B S Deepali
R Asokan
Y L Ramachandra
Riaz Mahmood
Lalitha Anand
author_facet Vageeshbabu S Hanur
D P Prakash
B S Deepali
R Asokan
Y L Ramachandra
Riaz Mahmood
Lalitha Anand
author_sort Vageeshbabu S Hanur
title Synergistic Use of Hypocotyl Explants and High Bap Preconditioning for Enhanced Transformation Frequency in Brinjal (Solanum melongena L.)
title_short Synergistic Use of Hypocotyl Explants and High Bap Preconditioning for Enhanced Transformation Frequency in Brinjal (Solanum melongena L.)
title_full Synergistic Use of Hypocotyl Explants and High Bap Preconditioning for Enhanced Transformation Frequency in Brinjal (Solanum melongena L.)
title_fullStr Synergistic Use of Hypocotyl Explants and High Bap Preconditioning for Enhanced Transformation Frequency in Brinjal (Solanum melongena L.)
title_full_unstemmed Synergistic Use of Hypocotyl Explants and High Bap Preconditioning for Enhanced Transformation Frequency in Brinjal (Solanum melongena L.)
title_sort synergistic use of hypocotyl explants and high bap preconditioning for enhanced transformation frequency in brinjal (solanum melongena l.)
publisher Society for Promotion of Horticulture - Indian Institute of Horticultural Research
publishDate 2006
url https://doaj.org/article/b49cfc24cd7e43539ed13648ae36c40b
work_keys_str_mv AT vageeshbabushanur synergisticuseofhypocotylexplantsandhighbappreconditioningforenhancedtransformationfrequencyinbrinjalsolanummelongenal
AT dpprakash synergisticuseofhypocotylexplantsandhighbappreconditioningforenhancedtransformationfrequencyinbrinjalsolanummelongenal
AT bsdeepali synergisticuseofhypocotylexplantsandhighbappreconditioningforenhancedtransformationfrequencyinbrinjalsolanummelongenal
AT rasokan synergisticuseofhypocotylexplantsandhighbappreconditioningforenhancedtransformationfrequencyinbrinjalsolanummelongenal
AT ylramachandra synergisticuseofhypocotylexplantsandhighbappreconditioningforenhancedtransformationfrequencyinbrinjalsolanummelongenal
AT riazmahmood synergisticuseofhypocotylexplantsandhighbappreconditioningforenhancedtransformationfrequencyinbrinjalsolanummelongenal
AT lalithaanand synergisticuseofhypocotylexplantsandhighbappreconditioningforenhancedtransformationfrequencyinbrinjalsolanummelongenal
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