A transcriptomic view to wounding response in young Scots pine stems

Abstract We studied the stress response of five-year-old Scots pine xylem to mechanical wounding using RNA sequencing. In general, we observed a bimodal response in pine xylem after wounding. Transcripts associated with water deficit stress, defence, and cell wall modification were induced at the ea...

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Autores principales:	Kean-Jin Lim, Tanja Paasela, Anni Harju, Martti Venäläinen, Lars Paulin, Petri Auvinen, Katri Kärkkäinen, Teemu H. Teeri
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b4a93d52c5264fbcac2793d78ab1ffb2
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spelling	oai:doaj.org-article:b4a93d52c5264fbcac2793d78ab1ffb22021-12-02T13:30:28ZA transcriptomic view to wounding response in young Scots pine stems10.1038/s41598-021-82848-32045-2322https://doaj.org/article/b4a93d52c5264fbcac2793d78ab1ffb22021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82848-3https://doaj.org/toc/2045-2322Abstract We studied the stress response of five-year-old Scots pine xylem to mechanical wounding using RNA sequencing. In general, we observed a bimodal response in pine xylem after wounding. Transcripts associated with water deficit stress, defence, and cell wall modification were induced at the earliest time point of three hours; at the same time, growth-related processes were down-regulated. A second temporal wave was triggered either at the middle and/or at the late time points (one and four days). Secondary metabolism, such as stilbene and lignan biosynthesis started one day after wounding. Scots pine synthesises the stilbenes pinosylvin and its monomethyl ether both as constitutive and induced defence compounds. Stilbene biosynthesis is induced by wounding, pathogens and UV stress, but is also developmentally regulated when heartwood is formed. Comparison of wounding responses to heartwood formation shows that many induced processes (in addition to stilbene biosynthesis) are similar and relate to defence or desiccation stress, but often specific transcripts are up-regulated in the developmental and wounding induced contexts. Pine resin biosynthesis was not induced in response to wounding, at least not during the first four days.Kean-Jin LimTanja PaaselaAnni HarjuMartti VenäläinenLars PaulinPetri AuvinenKatri KärkkäinenTeemu H. TeeriNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kean-Jin Lim Tanja Paasela Anni Harju Martti Venäläinen Lars Paulin Petri Auvinen Katri Kärkkäinen Teemu H. Teeri A transcriptomic view to wounding response in young Scots pine stems
description	Abstract We studied the stress response of five-year-old Scots pine xylem to mechanical wounding using RNA sequencing. In general, we observed a bimodal response in pine xylem after wounding. Transcripts associated with water deficit stress, defence, and cell wall modification were induced at the earliest time point of three hours; at the same time, growth-related processes were down-regulated. A second temporal wave was triggered either at the middle and/or at the late time points (one and four days). Secondary metabolism, such as stilbene and lignan biosynthesis started one day after wounding. Scots pine synthesises the stilbenes pinosylvin and its monomethyl ether both as constitutive and induced defence compounds. Stilbene biosynthesis is induced by wounding, pathogens and UV stress, but is also developmentally regulated when heartwood is formed. Comparison of wounding responses to heartwood formation shows that many induced processes (in addition to stilbene biosynthesis) are similar and relate to defence or desiccation stress, but often specific transcripts are up-regulated in the developmental and wounding induced contexts. Pine resin biosynthesis was not induced in response to wounding, at least not during the first four days.
format	article
author	Kean-Jin Lim Tanja Paasela Anni Harju Martti Venäläinen Lars Paulin Petri Auvinen Katri Kärkkäinen Teemu H. Teeri
author_facet	Kean-Jin Lim Tanja Paasela Anni Harju Martti Venäläinen Lars Paulin Petri Auvinen Katri Kärkkäinen Teemu H. Teeri
author_sort	Kean-Jin Lim
title	A transcriptomic view to wounding response in young Scots pine stems
title_short	A transcriptomic view to wounding response in young Scots pine stems
title_full	A transcriptomic view to wounding response in young Scots pine stems
title_fullStr	A transcriptomic view to wounding response in young Scots pine stems
title_full_unstemmed	A transcriptomic view to wounding response in young Scots pine stems
title_sort	transcriptomic view to wounding response in young scots pine stems
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/b4a93d52c5264fbcac2793d78ab1ffb2
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A transcriptomic view to wounding response in young Scots pine stems

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