Phenotypes, Lung Microbiota and Cytokine Responses in Pneumonia After Hematopoietic Stem Cell Transplantation

Yan Hu,1 Yanwen Jiang,1 Shuang Liu,1 Jiawei Shen,2 Youzhong An2 1Department of Respiratory and Critical Care Medicine, Peking University International Hospital, Beijing, People’s Republic of China; 2Department of Critical Care Medicine, Peking University People’s Hospital, Beijing, People’s Republic...

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Autores principales:	Hu Y, Jiang Y, Liu S, Shen J, An Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	hematopoietic stem cell transplantation pneumonia phenotype microbiota cytokines. Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/b4aa1337e5aa4cd88667efc3b4fbcfbf
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spelling	oai:doaj.org-article:b4aa1337e5aa4cd88667efc3b4fbcfbf2021-11-21T19:08:51ZPhenotypes, Lung Microbiota and Cytokine Responses in Pneumonia After Hematopoietic Stem Cell Transplantation1178-7031https://doaj.org/article/b4aa1337e5aa4cd88667efc3b4fbcfbf2021-11-01T00:00:00Zhttps://www.dovepress.com/phenotypes-lung-microbiota-and-cytokine-responses-in-pneumonia-after-h-peer-reviewed-fulltext-article-JIRhttps://doaj.org/toc/1178-7031Yan Hu,1 Yanwen Jiang,1 Shuang Liu,1 Jiawei Shen,2 Youzhong An2 1Department of Respiratory and Critical Care Medicine, Peking University International Hospital, Beijing, People’s Republic of China; 2Department of Critical Care Medicine, Peking University People’s Hospital, Beijing, People’s Republic of ChinaCorrespondence: Jiawei Shen; Youzhong AnDepartment of Critical Care Medicine, Peking University People’s Hospital, Beijing, People’s Republic of ChinaEmail jiaweishn@gmail.com; bjicu_bmc@163.comObjective: We aim to identify phenotypes of hematopoietic stem cell transplantation (HSCT) patients with pneumonia, discover relations of microbiota composition, cytokine profile, and outcomes between phenotypes. Specific cytokines will be evaluated for their role in lung injury in a murine model.Methods: HSCT patients with pneumonia were included, and clustering of variables including cytokine levels provided the phenotypes. Outcomes were compared between phenotypes. Analysis of lung microbiota identified marker species of phenotypes. In the murine model, marker species-related cytokine regulations and the role of cytokines in lung injury were evaluated.Results: Seventy-two patients were included, and two phenotypes were identified, namely “reactive” (N=21) and “nonreactive” (N=51) phenotype. Compared to their counterparts, patients with nonreactive phenotype had lower serum IL-6, IL-8, less severe inflammation, worse outcomes and more viruses as marker species in lung microbiota. The animal study validated the pathogens specific cytokine responses that presented in the human study and the potential protective role of IL-6 in these patients.Conclusion: HSCT patients with pneumonia can be clustered into two phenotypes with different marker species and outcomes: the “nonreactive” phenotype and the “reactive” phenotype. Serum cytokine levels were different between the two phenotypes, which indicate the existence of the pathogen-related cytokine responses. For patients with the “nonreactive” phenotype, IL-6 therapy may improve their prognosis, which should be further tested in clinical studies.Keywords: hematopoietic stem cell transplantation, pneumonia, phenotype, microbiota, cytokinesHu YJiang YLiu SShen JAn YDove Medical Pressarticlehematopoietic stem cell transplantationpneumoniaphenotypemicrobiotacytokines.PathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 6055-6065 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hematopoietic stem cell transplantation pneumonia phenotype microbiota cytokines. Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	hematopoietic stem cell transplantation pneumonia phenotype microbiota cytokines. Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Hu Y Jiang Y Liu S Shen J An Y Phenotypes, Lung Microbiota and Cytokine Responses in Pneumonia After Hematopoietic Stem Cell Transplantation
description	Yan Hu,1 Yanwen Jiang,1 Shuang Liu,1 Jiawei Shen,2 Youzhong An2 1Department of Respiratory and Critical Care Medicine, Peking University International Hospital, Beijing, People’s Republic of China; 2Department of Critical Care Medicine, Peking University People’s Hospital, Beijing, People’s Republic of ChinaCorrespondence: Jiawei Shen; Youzhong AnDepartment of Critical Care Medicine, Peking University People’s Hospital, Beijing, People’s Republic of ChinaEmail jiaweishn@gmail.com; bjicu_bmc@163.comObjective: We aim to identify phenotypes of hematopoietic stem cell transplantation (HSCT) patients with pneumonia, discover relations of microbiota composition, cytokine profile, and outcomes between phenotypes. Specific cytokines will be evaluated for their role in lung injury in a murine model.Methods: HSCT patients with pneumonia were included, and clustering of variables including cytokine levels provided the phenotypes. Outcomes were compared between phenotypes. Analysis of lung microbiota identified marker species of phenotypes. In the murine model, marker species-related cytokine regulations and the role of cytokines in lung injury were evaluated.Results: Seventy-two patients were included, and two phenotypes were identified, namely “reactive” (N=21) and “nonreactive” (N=51) phenotype. Compared to their counterparts, patients with nonreactive phenotype had lower serum IL-6, IL-8, less severe inflammation, worse outcomes and more viruses as marker species in lung microbiota. The animal study validated the pathogens specific cytokine responses that presented in the human study and the potential protective role of IL-6 in these patients.Conclusion: HSCT patients with pneumonia can be clustered into two phenotypes with different marker species and outcomes: the “nonreactive” phenotype and the “reactive” phenotype. Serum cytokine levels were different between the two phenotypes, which indicate the existence of the pathogen-related cytokine responses. For patients with the “nonreactive” phenotype, IL-6 therapy may improve their prognosis, which should be further tested in clinical studies.Keywords: hematopoietic stem cell transplantation, pneumonia, phenotype, microbiota, cytokines
format	article
author	Hu Y Jiang Y Liu S Shen J An Y
author_facet	Hu Y Jiang Y Liu S Shen J An Y
author_sort	Hu Y
title	Phenotypes, Lung Microbiota and Cytokine Responses in Pneumonia After Hematopoietic Stem Cell Transplantation
title_short	Phenotypes, Lung Microbiota and Cytokine Responses in Pneumonia After Hematopoietic Stem Cell Transplantation
title_full	Phenotypes, Lung Microbiota and Cytokine Responses in Pneumonia After Hematopoietic Stem Cell Transplantation
title_fullStr	Phenotypes, Lung Microbiota and Cytokine Responses in Pneumonia After Hematopoietic Stem Cell Transplantation
title_full_unstemmed	Phenotypes, Lung Microbiota and Cytokine Responses in Pneumonia After Hematopoietic Stem Cell Transplantation
title_sort	phenotypes, lung microbiota and cytokine responses in pneumonia after hematopoietic stem cell transplantation
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/b4aa1337e5aa4cd88667efc3b4fbcfbf
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_version_	1718418721478803456

Phenotypes, Lung Microbiota and Cytokine Responses in Pneumonia After Hematopoietic Stem Cell Transplantation

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