Silver-enhanced lateral flow immunoassay for highly-sensitive detection of potato leafroll virus
Rapid non-laboratory screening of plants for pathogenic viruses crucially influences crop yields in modern agricultural technologies. The aim of this study was to develop a highly-sensitive lateral flow immunoassay (LFIA) for rapid detection of potato leafroll virus (PLRV), an infectious agent of on...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Taylor & Francis Group
2018
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/b4b87754839243bbb1ffc4bbda13f71e |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:b4b87754839243bbb1ffc4bbda13f71e |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:b4b87754839243bbb1ffc4bbda13f71e2021-11-26T11:19:46ZSilver-enhanced lateral flow immunoassay for highly-sensitive detection of potato leafroll virus0954-01051465-344310.1080/09540105.2017.1401044https://doaj.org/article/b4b87754839243bbb1ffc4bbda13f71e2018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2017.1401044https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443Rapid non-laboratory screening of plants for pathogenic viruses crucially influences crop yields in modern agricultural technologies. The aim of this study was to develop a highly-sensitive lateral flow immunoassay (LFIA) for rapid detection of potato leafroll virus (PLRV), an infectious agent of one of the most widespread potato diseases. The proposed LFIA combines the formation of sandwich immune complexes with gold nanoparticles (GNP) as labels and silver enhancement. The enhancement stage was realized using mixture of silver lactate and hydroquinone and subsequent addition of chloride-containing buffer to stop silver reduction. LFIA with silver enhancement was 15 times more sensitive (detection limit 0.2 ng/mL; 15 min) compared with conventional LFIA (detection limit 3 ng/mL; 10 min). The enhanced LFIA detected PLRV in leaves’ extracts of infected potato in dilutions higher than enzyme-linked immunosorbent assay.Vasily G. PanferovIrina V. SafenkovaNadezhda A. ByzovaYuri A. VaritsevAnatoly V. ZherdevBoris B. DzantievTaylor & Francis Grouparticlegold nanoparticleslateral flow immunoassaysilver enhancementpotato leafroll viruspotato infectionsAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 445-457 (2018) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
gold nanoparticles lateral flow immunoassay silver enhancement potato leafroll virus potato infections Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 |
| spellingShingle |
gold nanoparticles lateral flow immunoassay silver enhancement potato leafroll virus potato infections Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Vasily G. Panferov Irina V. Safenkova Nadezhda A. Byzova Yuri A. Varitsev Anatoly V. Zherdev Boris B. Dzantiev Silver-enhanced lateral flow immunoassay for highly-sensitive detection of potato leafroll virus |
| description |
Rapid non-laboratory screening of plants for pathogenic viruses crucially influences crop yields in modern agricultural technologies. The aim of this study was to develop a highly-sensitive lateral flow immunoassay (LFIA) for rapid detection of potato leafroll virus (PLRV), an infectious agent of one of the most widespread potato diseases. The proposed LFIA combines the formation of sandwich immune complexes with gold nanoparticles (GNP) as labels and silver enhancement. The enhancement stage was realized using mixture of silver lactate and hydroquinone and subsequent addition of chloride-containing buffer to stop silver reduction. LFIA with silver enhancement was 15 times more sensitive (detection limit 0.2 ng/mL; 15 min) compared with conventional LFIA (detection limit 3 ng/mL; 10 min). The enhanced LFIA detected PLRV in leaves’ extracts of infected potato in dilutions higher than enzyme-linked immunosorbent assay. |
| format |
article |
| author |
Vasily G. Panferov Irina V. Safenkova Nadezhda A. Byzova Yuri A. Varitsev Anatoly V. Zherdev Boris B. Dzantiev |
| author_facet |
Vasily G. Panferov Irina V. Safenkova Nadezhda A. Byzova Yuri A. Varitsev Anatoly V. Zherdev Boris B. Dzantiev |
| author_sort |
Vasily G. Panferov |
| title |
Silver-enhanced lateral flow immunoassay for highly-sensitive detection of potato leafroll virus |
| title_short |
Silver-enhanced lateral flow immunoassay for highly-sensitive detection of potato leafroll virus |
| title_full |
Silver-enhanced lateral flow immunoassay for highly-sensitive detection of potato leafroll virus |
| title_fullStr |
Silver-enhanced lateral flow immunoassay for highly-sensitive detection of potato leafroll virus |
| title_full_unstemmed |
Silver-enhanced lateral flow immunoassay for highly-sensitive detection of potato leafroll virus |
| title_sort |
silver-enhanced lateral flow immunoassay for highly-sensitive detection of potato leafroll virus |
| publisher |
Taylor & Francis Group |
| publishDate |
2018 |
| url |
https://doaj.org/article/b4b87754839243bbb1ffc4bbda13f71e |
| work_keys_str_mv |
AT vasilygpanferov silverenhancedlateralflowimmunoassayforhighlysensitivedetectionofpotatoleafrollvirus AT irinavsafenkova silverenhancedlateralflowimmunoassayforhighlysensitivedetectionofpotatoleafrollvirus AT nadezhdaabyzova silverenhancedlateralflowimmunoassayforhighlysensitivedetectionofpotatoleafrollvirus AT yuriavaritsev silverenhancedlateralflowimmunoassayforhighlysensitivedetectionofpotatoleafrollvirus AT anatolyvzherdev silverenhancedlateralflowimmunoassayforhighlysensitivedetectionofpotatoleafrollvirus AT borisbdzantiev silverenhancedlateralflowimmunoassayforhighlysensitivedetectionofpotatoleafrollvirus |
| _version_ |
1718409556489404416 |