A Simplified and Efficient Method for Himar-1 Transposon Sequencing in Bacteria, Demonstrated by Creation and Analysis of a Saturated Transposon-Mutant Library in <named-content content-type="genus-species">Mycobacterium abscessus</named-content>
ABSTRACT We present a technically simple, easy-to-perform method for generating the genomic libraries for Himar-1 transposon site sequencing (Tn-seq). In addition to being simpler than present methods in the technical aspect, it also allows more robust and straightforward identification of the inser...
Saved in:
Main Authors: | Mark Foreman, Moran Gershoni, Daniel Barkan |
---|---|
Format: | article |
Language: | EN |
Published: |
American Society for Microbiology
2020
|
Subjects: | |
Online Access: | https://doaj.org/article/b4be4e940148445aac5ec82e79ab3f5b |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Development of a Counterselectable Transposon To Create Markerless Knockouts from an 18,432-Clone Ordered <named-content content-type="genus-species">Mycobacterium bovis</named-content> Bacillus Calmette-Guérin Mutant Resource
by: Katlyn Borgers, et al.
Published: (2020) -
Dual β-Lactam Combinations Highly Active against <named-content content-type="genus-species">Mycobacterium abscessus</named-content> Complex <italic toggle="yes">In Vitro</italic>
by: R. Pandey, et al.
Published: (2019) -
RETROSPECTIVE ANALYSIS OF SLOVENIAN MYCOBACTERIUM AVIUM COMPLEX AND MYCOBACTERIUM ABSCESSUS COMPLEX ISOLATES AND MOLECULAR RESISTANCE PROFILE
by: S. Truden, et al.
Published: (2018) -
Transposon Mutagenesis in <named-content content-type="genus-species">Chlamydia trachomatis</named-content> Identifies CT339 as a ComEC Homolog Important for DNA Uptake and Lateral Gene Transfer
by: Scott D. LaBrie, et al.
Published: (2019) -
Global Assessment of <named-content content-type="genus-species">Mycobacterium avium</named-content> subsp. <italic toggle="yes">hominissuis</italic> Genetic Requirement for Growth and Virulence
by: Marte S. Dragset, et al.
Published: (2019)