A Simplified and Efficient Method for Himar-1 Transposon Sequencing in Bacteria, Demonstrated by Creation and Analysis of a Saturated Transposon-Mutant Library in <named-content content-type="genus-species">Mycobacterium abscessus</named-content>
ABSTRACT We present a technically simple, easy-to-perform method for generating the genomic libraries for Himar-1 transposon site sequencing (Tn-seq). In addition to being simpler than present methods in the technical aspect, it also allows more robust and straightforward identification of the inser...
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| Auteurs principaux: | , , |
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| Format: | article |
| Langue: | EN |
| Publié: |
American Society for Microbiology
2020
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| Sujets: | |
| Accès en ligne: | https://doaj.org/article/b4be4e940148445aac5ec82e79ab3f5b |
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