Poly(A) RNAs including coding proteins RNAs occur in plant Cajal bodies.

The localisation of poly(A) RNA in plant cells containing either reticular (Allium cepa) or chromocentric (Lupinus luteus, Arabidopsis thaliana) nuclei was studied through in situ hybridisation. In both types of nuclei, the amount of poly(A) RNA was much greater in the nucleus than in the cytoplasm....

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Janusz Niedojadło, Ewa Kubicka, Beata Kalich, Dariusz J Smoliński
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b4c875753f904a8bacd378d18035d309
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:b4c875753f904a8bacd378d18035d309
record_format	dspace
spelling	oai:doaj.org-article:b4c875753f904a8bacd378d18035d3092021-11-25T05:54:44ZPoly(A) RNAs including coding proteins RNAs occur in plant Cajal bodies.1932-620310.1371/journal.pone.0111780https://doaj.org/article/b4c875753f904a8bacd378d18035d3092014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0111780https://doaj.org/toc/1932-6203The localisation of poly(A) RNA in plant cells containing either reticular (Allium cepa) or chromocentric (Lupinus luteus, Arabidopsis thaliana) nuclei was studied through in situ hybridisation. In both types of nuclei, the amount of poly(A) RNA was much greater in the nucleus than in the cytoplasm. In the nuclei, poly(A) RNA was present in structures resembling nuclear bodies. The molecular composition as well as the characteristic ultrastructure of the bodies containing poly(A) RNA demonstrated that they were Cajal bodies. We showed that some poly(A) RNAs in Cajal bodies code for proteins. However, examination of the localisation of active RNA polymerase II and in situ run-on transcription assays both demonstrated that CBs are not sites of transcription and that BrU-containing RNA accumulates in these structures long after synthesis. In addition, it was demonstrated that accumulation of poly(A) RNA occurs in the nuclei and CBs of hypoxia-treated cells. Our findings indicated that CBs may be involved in the later stages of poly(A) RNA metabolism, playing a role storage or retention.Janusz NiedojadłoEwa KubickaBeata KalichDariusz J SmolińskiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 11, p e111780 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Janusz Niedojadło Ewa Kubicka Beata Kalich Dariusz J Smoliński Poly(A) RNAs including coding proteins RNAs occur in plant Cajal bodies.
description	The localisation of poly(A) RNA in plant cells containing either reticular (Allium cepa) or chromocentric (Lupinus luteus, Arabidopsis thaliana) nuclei was studied through in situ hybridisation. In both types of nuclei, the amount of poly(A) RNA was much greater in the nucleus than in the cytoplasm. In the nuclei, poly(A) RNA was present in structures resembling nuclear bodies. The molecular composition as well as the characteristic ultrastructure of the bodies containing poly(A) RNA demonstrated that they were Cajal bodies. We showed that some poly(A) RNAs in Cajal bodies code for proteins. However, examination of the localisation of active RNA polymerase II and in situ run-on transcription assays both demonstrated that CBs are not sites of transcription and that BrU-containing RNA accumulates in these structures long after synthesis. In addition, it was demonstrated that accumulation of poly(A) RNA occurs in the nuclei and CBs of hypoxia-treated cells. Our findings indicated that CBs may be involved in the later stages of poly(A) RNA metabolism, playing a role storage or retention.
format	article
author	Janusz Niedojadło Ewa Kubicka Beata Kalich Dariusz J Smoliński
author_facet	Janusz Niedojadło Ewa Kubicka Beata Kalich Dariusz J Smoliński
author_sort	Janusz Niedojadło
title	Poly(A) RNAs including coding proteins RNAs occur in plant Cajal bodies.
title_short	Poly(A) RNAs including coding proteins RNAs occur in plant Cajal bodies.
title_full	Poly(A) RNAs including coding proteins RNAs occur in plant Cajal bodies.
title_fullStr	Poly(A) RNAs including coding proteins RNAs occur in plant Cajal bodies.
title_full_unstemmed	Poly(A) RNAs including coding proteins RNAs occur in plant Cajal bodies.
title_sort	poly(a) rnas including coding proteins rnas occur in plant cajal bodies.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/b4c875753f904a8bacd378d18035d309
work_keys_str_mv	AT januszniedojadło polyarnasincludingcodingproteinsrnasoccurinplantcajalbodies AT ewakubicka polyarnasincludingcodingproteinsrnasoccurinplantcajalbodies AT beatakalich polyarnasincludingcodingproteinsrnasoccurinplantcajalbodies AT dariuszjsmolinski polyarnasincludingcodingproteinsrnasoccurinplantcajalbodies
_version_	1718414419932741632

Poly(A) RNAs including coding proteins RNAs occur in plant Cajal bodies.

Ejemplares similares