Characterization of Endothelial Cells Associated with Cerebral Arteriovenous Malformation

Yu-Chen Jia,1,* Jia-Yue Fu,2,* Ping Huang,3 Zhan-Pu Zhang,3 Bo Chao,3 Jie Bai3 1Inner Mongolia Key Laboratory of Molecular Biology, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot, People’s Republic of China; 2Inner Mongolia Medical University, Hohhot, People&am...

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Autores principales:	Jia YC, Fu JY, Huang P, Zhang ZP, Chao B, Bai J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	cerebral arteriovenous malformation endothelial cell angiogenesis vegf Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/b4d1cfab39c9491bb274ed3b002ab6a8
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spelling	oai:doaj.org-article:b4d1cfab39c9491bb274ed3b002ab6a82021-12-02T09:54:29ZCharacterization of Endothelial Cells Associated with Cerebral Arteriovenous Malformation1178-2021https://doaj.org/article/b4d1cfab39c9491bb274ed3b002ab6a82020-04-01T00:00:00Zhttps://www.dovepress.com/characterization-of-endothelial-cells-associated-with-cerebral-arterio-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Yu-Chen Jia,1,* Jia-Yue Fu,2,* Ping Huang,3 Zhan-Pu Zhang,3 Bo Chao,3 Jie Bai3 1Inner Mongolia Key Laboratory of Molecular Biology, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot, People’s Republic of China; 2Inner Mongolia Medical University, Hohhot, People’s Republic of China; 3Department of Neurosurgery, Affiliated Hospital, Inner Mongolia Medical University, Hohhot, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jie BaiDepartment of Neurosurgery, Affiliated Hospital, Inner Mongolia Medical University, No. 1 Tongdao North Road, Hohhot City, Inner Mongolia 010000, People’s Republic of ChinaTel +86-471-6351352Fax +86-471-6351184Email baijie0611@126.comIntroduction: Cerebral arteriovenous malformation (cAVM) is a disease characterized by the angiogenesis and remodeling of veins. However, whether vascular endothelial cells (ECs) exhibit morphological and functional changes during cAVM remains unclear. This study aimed to investigate the role of ECs in the pathogenesis of cAVM.Methods: Rat model of cAVM was established by anastomosing the common carotid artery with the external jugular vein. The digital subtraction angiography (DSA), HE, Masson and immunohistochemical staining were performed to evaluate the model. ECs were isolated from AVM rat model or control rats, and characterized by MTT, cell scratch, and tube formation assays. The secretion of vascular endothelial growth factor (VEGF) was detected by ELISA.Results: AVM rat model showed typical pathological characteristics of cAVM. In addition, the proliferation, migration and tube formation abilities of ECs of arterialized vein (AV-ECs) were significantly better than those of ECs of normal vein (NV-ECs). Moreover, the levels of secreted VEGF were significantly higher in AV-ECs than in NV-ECs.Conclusion: AV-ECs isolated from AVM rat model showed increased proliferation, migration and angiogenesis and may be potential target for the treatment of cAVM.Keywords: cerebral arteriovenous malformation, endothelial cell, angiogenesis, VEGFJia YCFu JYHuang PZhang ZPChao BBai JDove Medical Pressarticlecerebral arteriovenous malformationendothelial cellangiogenesisvegfNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 16, Pp 1015-1022 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	cerebral arteriovenous malformation endothelial cell angiogenesis vegf Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	cerebral arteriovenous malformation endothelial cell angiogenesis vegf Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Jia YC Fu JY Huang P Zhang ZP Chao B Bai J Characterization of Endothelial Cells Associated with Cerebral Arteriovenous Malformation
description	Yu-Chen Jia,1,* Jia-Yue Fu,2,* Ping Huang,3 Zhan-Pu Zhang,3 Bo Chao,3 Jie Bai3 1Inner Mongolia Key Laboratory of Molecular Biology, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot, People’s Republic of China; 2Inner Mongolia Medical University, Hohhot, People’s Republic of China; 3Department of Neurosurgery, Affiliated Hospital, Inner Mongolia Medical University, Hohhot, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jie BaiDepartment of Neurosurgery, Affiliated Hospital, Inner Mongolia Medical University, No. 1 Tongdao North Road, Hohhot City, Inner Mongolia 010000, People’s Republic of ChinaTel +86-471-6351352Fax +86-471-6351184Email baijie0611@126.comIntroduction: Cerebral arteriovenous malformation (cAVM) is a disease characterized by the angiogenesis and remodeling of veins. However, whether vascular endothelial cells (ECs) exhibit morphological and functional changes during cAVM remains unclear. This study aimed to investigate the role of ECs in the pathogenesis of cAVM.Methods: Rat model of cAVM was established by anastomosing the common carotid artery with the external jugular vein. The digital subtraction angiography (DSA), HE, Masson and immunohistochemical staining were performed to evaluate the model. ECs were isolated from AVM rat model or control rats, and characterized by MTT, cell scratch, and tube formation assays. The secretion of vascular endothelial growth factor (VEGF) was detected by ELISA.Results: AVM rat model showed typical pathological characteristics of cAVM. In addition, the proliferation, migration and tube formation abilities of ECs of arterialized vein (AV-ECs) were significantly better than those of ECs of normal vein (NV-ECs). Moreover, the levels of secreted VEGF were significantly higher in AV-ECs than in NV-ECs.Conclusion: AV-ECs isolated from AVM rat model showed increased proliferation, migration and angiogenesis and may be potential target for the treatment of cAVM.Keywords: cerebral arteriovenous malformation, endothelial cell, angiogenesis, VEGF
format	article
author	Jia YC Fu JY Huang P Zhang ZP Chao B Bai J
author_facet	Jia YC Fu JY Huang P Zhang ZP Chao B Bai J
author_sort	Jia YC
title	Characterization of Endothelial Cells Associated with Cerebral Arteriovenous Malformation
title_short	Characterization of Endothelial Cells Associated with Cerebral Arteriovenous Malformation
title_full	Characterization of Endothelial Cells Associated with Cerebral Arteriovenous Malformation
title_fullStr	Characterization of Endothelial Cells Associated with Cerebral Arteriovenous Malformation
title_full_unstemmed	Characterization of Endothelial Cells Associated with Cerebral Arteriovenous Malformation
title_sort	characterization of endothelial cells associated with cerebral arteriovenous malformation
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/b4d1cfab39c9491bb274ed3b002ab6a8
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Characterization of Endothelial Cells Associated with Cerebral Arteriovenous Malformation

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