Blood feeding and Plasmodium infection alters the miRNome of Anopheles stephensi.

Blood feeding is an integral process required for physiological functions and propagation of the malaria vector Anopheles. During blood feeding, presence of the malaria parasite, Plasmodium in the blood induces several host effector molecules including microRNAs which play important roles in the dev...

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Autores principales:	Shanu Jain, Vandita Rana, Jatin Shrinet, Anil Sharma, Adak Tridibes, Sujatha Sunil, Raj K Bhatnagar
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b4d61eb0aaf7478bb35308ad2cce782d
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spelling	oai:doaj.org-article:b4d61eb0aaf7478bb35308ad2cce782d2021-11-18T08:18:04ZBlood feeding and Plasmodium infection alters the miRNome of Anopheles stephensi.1932-620310.1371/journal.pone.0098402https://doaj.org/article/b4d61eb0aaf7478bb35308ad2cce782d2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24866389/?tool=EBIhttps://doaj.org/toc/1932-6203Blood feeding is an integral process required for physiological functions and propagation of the malaria vector Anopheles. During blood feeding, presence of the malaria parasite, Plasmodium in the blood induces several host effector molecules including microRNAs which play important roles in the development and maturation of the parasite within the mosquito. The present study was undertaken to elucidate the dynamic expression of miRNAs during gonotrophic cycle and parasite development in Anopheles stephensi. Using next generation sequencing technology, we identified 126 miRNAs of which 17 were novel miRNAs. The miRNAs were further validated by northern hybridization and cloning. Blood feeding and parasitized blood feeding in the mosquitoes revealed regulation of 13 and 16 miRNAs respectively. Expression profiling of these miRNAs revealed that significant miRNAs were down-regulated upon parasitized blood feeding with a repertoire of miRNAs showing stage specific up-regulation. Expression profiles of significantly modulated miRNAs were further validated by real time PCR. Target prediction of regulated miRNAs revealed overlapping targeting by different miRNAs. These targets included several metabolic pathways including metabolic, redox homeostasis and protein processing machinery components. Our analysis revealed tight regulation of specific miRNAs post blood feeding and parasite infection in An. stephensi. Such regulated expression suggests possible role of these miRNAs during gonotrophic cycle in mosquito. Another set of miRNAs were also significantly regulated at 42 h and 5 days post infection indicating parasite stage-specific role of host miRNAs. This study will result in better understanding of the role of miRNAs during gonotrophic cycle and parasite development in mosquito and can probably facilitate in devising novel malaria control strategies at vector level.Shanu JainVandita RanaJatin ShrinetAnil SharmaAdak TridibesSujatha SunilRaj K BhatnagarPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 5, p e98402 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shanu Jain Vandita Rana Jatin Shrinet Anil Sharma Adak Tridibes Sujatha Sunil Raj K Bhatnagar Blood feeding and Plasmodium infection alters the miRNome of Anopheles stephensi.
description	Blood feeding is an integral process required for physiological functions and propagation of the malaria vector Anopheles. During blood feeding, presence of the malaria parasite, Plasmodium in the blood induces several host effector molecules including microRNAs which play important roles in the development and maturation of the parasite within the mosquito. The present study was undertaken to elucidate the dynamic expression of miRNAs during gonotrophic cycle and parasite development in Anopheles stephensi. Using next generation sequencing technology, we identified 126 miRNAs of which 17 were novel miRNAs. The miRNAs were further validated by northern hybridization and cloning. Blood feeding and parasitized blood feeding in the mosquitoes revealed regulation of 13 and 16 miRNAs respectively. Expression profiling of these miRNAs revealed that significant miRNAs were down-regulated upon parasitized blood feeding with a repertoire of miRNAs showing stage specific up-regulation. Expression profiles of significantly modulated miRNAs were further validated by real time PCR. Target prediction of regulated miRNAs revealed overlapping targeting by different miRNAs. These targets included several metabolic pathways including metabolic, redox homeostasis and protein processing machinery components. Our analysis revealed tight regulation of specific miRNAs post blood feeding and parasite infection in An. stephensi. Such regulated expression suggests possible role of these miRNAs during gonotrophic cycle in mosquito. Another set of miRNAs were also significantly regulated at 42 h and 5 days post infection indicating parasite stage-specific role of host miRNAs. This study will result in better understanding of the role of miRNAs during gonotrophic cycle and parasite development in mosquito and can probably facilitate in devising novel malaria control strategies at vector level.
format	article
author	Shanu Jain Vandita Rana Jatin Shrinet Anil Sharma Adak Tridibes Sujatha Sunil Raj K Bhatnagar
author_facet	Shanu Jain Vandita Rana Jatin Shrinet Anil Sharma Adak Tridibes Sujatha Sunil Raj K Bhatnagar
author_sort	Shanu Jain
title	Blood feeding and Plasmodium infection alters the miRNome of Anopheles stephensi.
title_short	Blood feeding and Plasmodium infection alters the miRNome of Anopheles stephensi.
title_full	Blood feeding and Plasmodium infection alters the miRNome of Anopheles stephensi.
title_fullStr	Blood feeding and Plasmodium infection alters the miRNome of Anopheles stephensi.
title_full_unstemmed	Blood feeding and Plasmodium infection alters the miRNome of Anopheles stephensi.
title_sort	blood feeding and plasmodium infection alters the mirnome of anopheles stephensi.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/b4d61eb0aaf7478bb35308ad2cce782d
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Blood feeding and Plasmodium infection alters the miRNome of Anopheles stephensi.

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