Obesity in Children with Leptin Receptor Gene Polymorphisms

Introduction: The study of single nucleotide polymorphisms (SNPs) of the leptin receptor gene (LEPR) based on next generation genomic sequencing (NGS) data is becoming an increasingly important aspect of diagnosis, treatment and prevention of both metabolically healthy (MHO) and metabolically unheal...

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Autores principales:	Aleksandr Abaturov, Anna Nikulina
Formato:	article
Lenguaje:	EN
Publicado:	Karolinum Press 2021
Materias:	Medicine R
Acceso en línea:	https://doaj.org/article/b4d66ffc54a54251b35e51937e3d097b
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spelling	oai:doaj.org-article:b4d66ffc54a54251b35e51937e3d097b2021-11-22T09:31:01ZObesity in Children with Leptin Receptor Gene Polymorphisms1805-96941211-428610.14712/18059694.2021.27https://doaj.org/article/b4d66ffc54a54251b35e51937e3d097b2021-11-01T00:00:00Zhttps://actamedica.lfhk.cuni.cz/64/3/0158/https://doaj.org/toc/1211-4286https://doaj.org/toc/1805-9694Introduction: The study of single nucleotide polymorphisms (SNPs) of the leptin receptor gene (LEPR) based on next generation genomic sequencing (NGS) data is becoming an increasingly important aspect of diagnosis, treatment and prevention of both metabolically healthy (MHO) and metabolically unhealthy obesity (MUO) phenotypes. Material and methods: 35 obese children 6-18 years old were examined by the NGS method with bioinformatic analysis. The main group (n = 18) was formed by children with MUO, according to the recommendations of the expert group of the National Heart, Lung, and Blood Institute. The control group (n = 17) was represented by children with MHO. Statistical methods were used: analysis of variance, Wald’s sequential analysis, Spearman’s correlation analysis, analysis of nominal data and multiple discriminant analysis. Results: 10 types of non-synonymous SNPs (rs3790435, rs1137100, rs2186248, rs70940803, rs79639154, rs1359482195, rs1137101, rs1805094, rs13306520, rs13306522) of the LEPR gene in obese children have been identified. Multiple discriminant analysis demonstrated that the following LEPR SNPs are of greatest importance in the development of MUO: rs3790435, rs13306522, rs13306520. Analysis of nominal data revealed significant differences in the groups for Copy number variation (CNV) rs3790435 of the LEPR gene. Wald’s analysis allowed us to identify 6 important predictors of MUO (І ≥ 0.5): 2 CNV rs3790435 (Relative Risk, RR = 2, Prognostic coefficient, PC = +2.76); male gender of the child (RR = 1.3, PC = +1.35); rs3790435 (RR = 1.9, PC = +2.76); hyperleptinemia more than 40.56 ng/ml (RR = 2, PC = +3); CNV rs1359482195 ≥ 3 (RR = 1.9, PC = +5.8); SNP of the LEPR gene ≥4 (RR = 3.8, PC = +5.8). Conclusion: Children with the genotype rs3790435 gene LEPR had signs of MUO more often.Aleksandr AbaturovAnna NikulinaKarolinum PressarticleMedicineRENActa Medica, Vol 64, Iss 3, Pp 158-164 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R
spellingShingle	Medicine R Aleksandr Abaturov Anna Nikulina Obesity in Children with Leptin Receptor Gene Polymorphisms
description	Introduction: The study of single nucleotide polymorphisms (SNPs) of the leptin receptor gene (LEPR) based on next generation genomic sequencing (NGS) data is becoming an increasingly important aspect of diagnosis, treatment and prevention of both metabolically healthy (MHO) and metabolically unhealthy obesity (MUO) phenotypes. Material and methods: 35 obese children 6-18 years old were examined by the NGS method with bioinformatic analysis. The main group (n = 18) was formed by children with MUO, according to the recommendations of the expert group of the National Heart, Lung, and Blood Institute. The control group (n = 17) was represented by children with MHO. Statistical methods were used: analysis of variance, Wald’s sequential analysis, Spearman’s correlation analysis, analysis of nominal data and multiple discriminant analysis. Results: 10 types of non-synonymous SNPs (rs3790435, rs1137100, rs2186248, rs70940803, rs79639154, rs1359482195, rs1137101, rs1805094, rs13306520, rs13306522) of the LEPR gene in obese children have been identified. Multiple discriminant analysis demonstrated that the following LEPR SNPs are of greatest importance in the development of MUO: rs3790435, rs13306522, rs13306520. Analysis of nominal data revealed significant differences in the groups for Copy number variation (CNV) rs3790435 of the LEPR gene. Wald’s analysis allowed us to identify 6 important predictors of MUO (І ≥ 0.5): 2 CNV rs3790435 (Relative Risk, RR = 2, Prognostic coefficient, PC = +2.76); male gender of the child (RR = 1.3, PC = +1.35); rs3790435 (RR = 1.9, PC = +2.76); hyperleptinemia more than 40.56 ng/ml (RR = 2, PC = +3); CNV rs1359482195 ≥ 3 (RR = 1.9, PC = +5.8); SNP of the LEPR gene ≥4 (RR = 3.8, PC = +5.8). Conclusion: Children with the genotype rs3790435 gene LEPR had signs of MUO more often.
format	article
author	Aleksandr Abaturov Anna Nikulina
author_facet	Aleksandr Abaturov Anna Nikulina
author_sort	Aleksandr Abaturov
title	Obesity in Children with Leptin Receptor Gene Polymorphisms
title_short	Obesity in Children with Leptin Receptor Gene Polymorphisms
title_full	Obesity in Children with Leptin Receptor Gene Polymorphisms
title_fullStr	Obesity in Children with Leptin Receptor Gene Polymorphisms
title_full_unstemmed	Obesity in Children with Leptin Receptor Gene Polymorphisms
title_sort	obesity in children with leptin receptor gene polymorphisms
publisher	Karolinum Press
publishDate	2021
url	https://doaj.org/article/b4d66ffc54a54251b35e51937e3d097b
work_keys_str_mv	AT aleksandrabaturov obesityinchildrenwithleptinreceptorgenepolymorphisms AT annanikulina obesityinchildrenwithleptinreceptorgenepolymorphisms
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Obesity in Children with Leptin Receptor Gene Polymorphisms

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