Human intestinal cells modulate conjugational transfer of multidrug resistance plasmids between clinical Escherichia coli isolates.

Bacterial conjugation in the human gut microbiota is believed to play a major role in the dissemination of antibiotic resistance genes and virulence plasmids. However, the modulation of bacterial conjugation by the human host remains poorly understood and there is a need for controlled systems to st...

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Autores principales:	Ana Manuel Dantas Machado, Morten O A Sommer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b4dc25e063fd493997af954805c5f685
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spelling	oai:doaj.org-article:b4dc25e063fd493997af954805c5f6852021-11-18T08:14:44ZHuman intestinal cells modulate conjugational transfer of multidrug resistance plasmids between clinical Escherichia coli isolates.1932-620310.1371/journal.pone.0100739https://doaj.org/article/b4dc25e063fd493997af954805c5f6852014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24955767/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Bacterial conjugation in the human gut microbiota is believed to play a major role in the dissemination of antibiotic resistance genes and virulence plasmids. However, the modulation of bacterial conjugation by the human host remains poorly understood and there is a need for controlled systems to study this process. We established an in vitro co-culture system to study the interaction between human intestinal cells and bacteria. We show that the conjugation efficiency of a plasmid encoding an extended spectrum beta-lactamase is reduced when clinical isolates of Escherichia coli are co-cultured with human intestinal cells. We show that filtered media from co-cultures contain a factor that reduces conjugation efficiency. Protease treatment of the filtered media eliminates this inhibition of conjugation. This data suggests that a peptide or protein based factor is secreted on the apical side of the intestinal cells exposed to bacteria leading to a two-fold reduction in conjugation efficiency. These results show that human gut epithelial cells can modulate bacterial conjugation and may have relevance to gene exchange in the gut.Ana Manuel Dantas MachadoMorten O A SommerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e100739 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ana Manuel Dantas Machado Morten O A Sommer Human intestinal cells modulate conjugational transfer of multidrug resistance plasmids between clinical Escherichia coli isolates.
description	Bacterial conjugation in the human gut microbiota is believed to play a major role in the dissemination of antibiotic resistance genes and virulence plasmids. However, the modulation of bacterial conjugation by the human host remains poorly understood and there is a need for controlled systems to study this process. We established an in vitro co-culture system to study the interaction between human intestinal cells and bacteria. We show that the conjugation efficiency of a plasmid encoding an extended spectrum beta-lactamase is reduced when clinical isolates of Escherichia coli are co-cultured with human intestinal cells. We show that filtered media from co-cultures contain a factor that reduces conjugation efficiency. Protease treatment of the filtered media eliminates this inhibition of conjugation. This data suggests that a peptide or protein based factor is secreted on the apical side of the intestinal cells exposed to bacteria leading to a two-fold reduction in conjugation efficiency. These results show that human gut epithelial cells can modulate bacterial conjugation and may have relevance to gene exchange in the gut.
format	article
author	Ana Manuel Dantas Machado Morten O A Sommer
author_facet	Ana Manuel Dantas Machado Morten O A Sommer
author_sort	Ana Manuel Dantas Machado
title	Human intestinal cells modulate conjugational transfer of multidrug resistance plasmids between clinical Escherichia coli isolates.
title_short	Human intestinal cells modulate conjugational transfer of multidrug resistance plasmids between clinical Escherichia coli isolates.
title_full	Human intestinal cells modulate conjugational transfer of multidrug resistance plasmids between clinical Escherichia coli isolates.
title_fullStr	Human intestinal cells modulate conjugational transfer of multidrug resistance plasmids between clinical Escherichia coli isolates.
title_full_unstemmed	Human intestinal cells modulate conjugational transfer of multidrug resistance plasmids between clinical Escherichia coli isolates.
title_sort	human intestinal cells modulate conjugational transfer of multidrug resistance plasmids between clinical escherichia coli isolates.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/b4dc25e063fd493997af954805c5f685
work_keys_str_mv	AT anamanueldantasmachado humanintestinalcellsmodulateconjugationaltransferofmultidrugresistanceplasmidsbetweenclinicalescherichiacoliisolates AT mortenoasommer humanintestinalcellsmodulateconjugationaltransferofmultidrugresistanceplasmidsbetweenclinicalescherichiacoliisolates
_version_	1718422006128443392

Human intestinal cells modulate conjugational transfer of multidrug resistance plasmids between clinical Escherichia coli isolates.

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