The role of axonal Kv1 channels in CA3 pyramidal cell excitability

Abstract Axonal ion channels control spike initiation and propagation along the axon and determine action potential waveform. We show here that functional suppression of axonal Kv1 channels with local puff of dendrotoxin (DTx), laser or mechanical axotomy significantly increased excitability measure...

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Autores principales:	Sylvain Rama, Mickael Zbili, Aurélie Fékété, Mónica Tapia, Maria José Benitez, Norah Boumedine, Juan José Garrido, Dominique Debanne
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b4e80117a02f4e5d86fe9015fa2624b5
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spelling	oai:doaj.org-article:b4e80117a02f4e5d86fe9015fa2624b52021-12-02T15:05:16ZThe role of axonal Kv1 channels in CA3 pyramidal cell excitability10.1038/s41598-017-00388-12045-2322https://doaj.org/article/b4e80117a02f4e5d86fe9015fa2624b52017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00388-1https://doaj.org/toc/2045-2322Abstract Axonal ion channels control spike initiation and propagation along the axon and determine action potential waveform. We show here that functional suppression of axonal Kv1 channels with local puff of dendrotoxin (DTx), laser or mechanical axotomy significantly increased excitability measured in the cell body. Importantly, the functional effect of DTx puffing or axotomy was not limited to the axon initial segment but was also seen on axon collaterals. In contrast, no effects were observed when DTx was puffed on single apical dendrites or after single dendrotomy. A simple model with Kv1 located in the axon reproduced the experimental observations and showed that the distance at which the effects of axon collateral cuts are seen depends on the axon space constant. In conclusion, Kv1 channels located in the axon proper greatly participate in intrinsic excitability of CA3 pyramidal neurons. This finding stresses the importance of the axonal compartment in the regulation of intrinsic neuronal excitability.Sylvain RamaMickael ZbiliAurélie FékétéMónica TapiaMaria José BenitezNorah BoumedineJuan José GarridoDominique DebanneNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sylvain Rama Mickael Zbili Aurélie Fékété Mónica Tapia Maria José Benitez Norah Boumedine Juan José Garrido Dominique Debanne The role of axonal Kv1 channels in CA3 pyramidal cell excitability
description	Abstract Axonal ion channels control spike initiation and propagation along the axon and determine action potential waveform. We show here that functional suppression of axonal Kv1 channels with local puff of dendrotoxin (DTx), laser or mechanical axotomy significantly increased excitability measured in the cell body. Importantly, the functional effect of DTx puffing or axotomy was not limited to the axon initial segment but was also seen on axon collaterals. In contrast, no effects were observed when DTx was puffed on single apical dendrites or after single dendrotomy. A simple model with Kv1 located in the axon reproduced the experimental observations and showed that the distance at which the effects of axon collateral cuts are seen depends on the axon space constant. In conclusion, Kv1 channels located in the axon proper greatly participate in intrinsic excitability of CA3 pyramidal neurons. This finding stresses the importance of the axonal compartment in the regulation of intrinsic neuronal excitability.
format	article
author	Sylvain Rama Mickael Zbili Aurélie Fékété Mónica Tapia Maria José Benitez Norah Boumedine Juan José Garrido Dominique Debanne
author_facet	Sylvain Rama Mickael Zbili Aurélie Fékété Mónica Tapia Maria José Benitez Norah Boumedine Juan José Garrido Dominique Debanne
author_sort	Sylvain Rama
title	The role of axonal Kv1 channels in CA3 pyramidal cell excitability
title_short	The role of axonal Kv1 channels in CA3 pyramidal cell excitability
title_full	The role of axonal Kv1 channels in CA3 pyramidal cell excitability
title_fullStr	The role of axonal Kv1 channels in CA3 pyramidal cell excitability
title_full_unstemmed	The role of axonal Kv1 channels in CA3 pyramidal cell excitability
title_sort	role of axonal kv1 channels in ca3 pyramidal cell excitability
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/b4e80117a02f4e5d86fe9015fa2624b5
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The role of axonal Kv1 channels in CA3 pyramidal cell excitability

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