Integrated dual-tomography for refractive index analysis of free-floating single living cell with isotropic superresolution

Abstract Digital holographic microtomography is a promising technique for three-dimensional (3D) measurement of the refractive index (RI) profiles of biological specimens. Measurement of the RI distribution of a free-floating single living cell with an isotropic superresolution had not previously be...

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Autores principales: Vinoth B., Xin-Ji Lai, Yu-Chih Lin, Han-Yen Tu, Chau-Jern Cheng
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Lenguaje:EN
Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/b4ebdd09d8b94e7889ead319dd4bdd04
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spelling oai:doaj.org-article:b4ebdd09d8b94e7889ead319dd4bdd042021-12-02T15:07:59ZIntegrated dual-tomography for refractive index analysis of free-floating single living cell with isotropic superresolution10.1038/s41598-018-24408-w2045-2322https://doaj.org/article/b4ebdd09d8b94e7889ead319dd4bdd042018-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-24408-whttps://doaj.org/toc/2045-2322Abstract Digital holographic microtomography is a promising technique for three-dimensional (3D) measurement of the refractive index (RI) profiles of biological specimens. Measurement of the RI distribution of a free-floating single living cell with an isotropic superresolution had not previously been accomplished. To the best of our knowledge, this is the first study focusing on the development of an integrated dual-tomographic (IDT) imaging system for RI measurement of an unlabelled free-floating single living cell with an isotropic superresolution by combining the spatial frequencies of full-angle specimen rotation with those of beam rotation. A novel ‘UFO’ (unidentified flying object) like shaped coherent transfer function is obtained. The IDT imaging system does not require any complex image-processing algorithm for 3D reconstruction. The working principle was successfully demonstrated and a 3D RI profile of a single living cell, Candida rugosa, was obtained with an isotropic superresolution. This technology is expected to set a benchmark for free-floating single live sample measurements without labeling or any special sample preparations for the experiments.Vinoth B.Xin-Ji LaiYu-Chih LinHan-Yen TuChau-Jern ChengNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-7 (2018)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Vinoth B.
Xin-Ji Lai
Yu-Chih Lin
Han-Yen Tu
Chau-Jern Cheng
Integrated dual-tomography for refractive index analysis of free-floating single living cell with isotropic superresolution
description Abstract Digital holographic microtomography is a promising technique for three-dimensional (3D) measurement of the refractive index (RI) profiles of biological specimens. Measurement of the RI distribution of a free-floating single living cell with an isotropic superresolution had not previously been accomplished. To the best of our knowledge, this is the first study focusing on the development of an integrated dual-tomographic (IDT) imaging system for RI measurement of an unlabelled free-floating single living cell with an isotropic superresolution by combining the spatial frequencies of full-angle specimen rotation with those of beam rotation. A novel ‘UFO’ (unidentified flying object) like shaped coherent transfer function is obtained. The IDT imaging system does not require any complex image-processing algorithm for 3D reconstruction. The working principle was successfully demonstrated and a 3D RI profile of a single living cell, Candida rugosa, was obtained with an isotropic superresolution. This technology is expected to set a benchmark for free-floating single live sample measurements without labeling or any special sample preparations for the experiments.
format article
author Vinoth B.
Xin-Ji Lai
Yu-Chih Lin
Han-Yen Tu
Chau-Jern Cheng
author_facet Vinoth B.
Xin-Ji Lai
Yu-Chih Lin
Han-Yen Tu
Chau-Jern Cheng
author_sort Vinoth B.
title Integrated dual-tomography for refractive index analysis of free-floating single living cell with isotropic superresolution
title_short Integrated dual-tomography for refractive index analysis of free-floating single living cell with isotropic superresolution
title_full Integrated dual-tomography for refractive index analysis of free-floating single living cell with isotropic superresolution
title_fullStr Integrated dual-tomography for refractive index analysis of free-floating single living cell with isotropic superresolution
title_full_unstemmed Integrated dual-tomography for refractive index analysis of free-floating single living cell with isotropic superresolution
title_sort integrated dual-tomography for refractive index analysis of free-floating single living cell with isotropic superresolution
publisher Nature Portfolio
publishDate 2018
url https://doaj.org/article/b4ebdd09d8b94e7889ead319dd4bdd04
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