Activation of Wnt/β-catenin signalling affects differentiation of cells arising from the cerebellar ventricular zone.

Development of the cerebellum proceeds under the precise spatio-temporal control of several key developmental signalling pathways, including the Wnt/β-catenin pathway. We recently reported the activity of Wnt/β-catenin signalling in the perinatal cerebellar ventricular zone (VZ), a germinal centre i...

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Autores principales:	Hayden J Selvadurai, John O Mason
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b4f0766821304adc81a311a3fea18090
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spelling	oai:doaj.org-article:b4f0766821304adc81a311a3fea180902021-11-18T07:09:42ZActivation of Wnt/β-catenin signalling affects differentiation of cells arising from the cerebellar ventricular zone.1932-620310.1371/journal.pone.0042572https://doaj.org/article/b4f0766821304adc81a311a3fea180902012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22880037/?tool=EBIhttps://doaj.org/toc/1932-6203Development of the cerebellum proceeds under the precise spatio-temporal control of several key developmental signalling pathways, including the Wnt/β-catenin pathway. We recently reported the activity of Wnt/β-catenin signalling in the perinatal cerebellar ventricular zone (VZ), a germinal centre in the developing cerebellum that gives rise to GABAergic and glial cells. In order to investigate the normal function of Wnt/β-catenin signalling in the VZ and the cell lineages it gives rise to, we used a combination of ex vivo cerebellar slice culture and in vivo genetic manipulation to dysregulate its activity during late embryonic development. Activation of the pathway at the cerebellar ventricular zone led to a reduction in the number of cells expressing the glial lineage markers Sox9 and GFAP and the interneuron marker Pax2, but had no consistent effect on either proliferation or apoptosis. Our findings suggest that activation of the Wnt/β-catenin pathway in the cerebellar ventricular zone causes a shift in the cell types produced, most likely due to disruption of normal differentiation. Thus, we propose that regulation of Wnt/β-catenin signalling levels are required for normal development of cells arising from the cerebellar ventricular zone during late embryogenesis.Hayden J SelvaduraiJohn O MasonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e42572 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hayden J Selvadurai John O Mason Activation of Wnt/β-catenin signalling affects differentiation of cells arising from the cerebellar ventricular zone.
description	Development of the cerebellum proceeds under the precise spatio-temporal control of several key developmental signalling pathways, including the Wnt/β-catenin pathway. We recently reported the activity of Wnt/β-catenin signalling in the perinatal cerebellar ventricular zone (VZ), a germinal centre in the developing cerebellum that gives rise to GABAergic and glial cells. In order to investigate the normal function of Wnt/β-catenin signalling in the VZ and the cell lineages it gives rise to, we used a combination of ex vivo cerebellar slice culture and in vivo genetic manipulation to dysregulate its activity during late embryonic development. Activation of the pathway at the cerebellar ventricular zone led to a reduction in the number of cells expressing the glial lineage markers Sox9 and GFAP and the interneuron marker Pax2, but had no consistent effect on either proliferation or apoptosis. Our findings suggest that activation of the Wnt/β-catenin pathway in the cerebellar ventricular zone causes a shift in the cell types produced, most likely due to disruption of normal differentiation. Thus, we propose that regulation of Wnt/β-catenin signalling levels are required for normal development of cells arising from the cerebellar ventricular zone during late embryogenesis.
format	article
author	Hayden J Selvadurai John O Mason
author_facet	Hayden J Selvadurai John O Mason
author_sort	Hayden J Selvadurai
title	Activation of Wnt/β-catenin signalling affects differentiation of cells arising from the cerebellar ventricular zone.
title_short	Activation of Wnt/β-catenin signalling affects differentiation of cells arising from the cerebellar ventricular zone.
title_full	Activation of Wnt/β-catenin signalling affects differentiation of cells arising from the cerebellar ventricular zone.
title_fullStr	Activation of Wnt/β-catenin signalling affects differentiation of cells arising from the cerebellar ventricular zone.
title_full_unstemmed	Activation of Wnt/β-catenin signalling affects differentiation of cells arising from the cerebellar ventricular zone.
title_sort	activation of wnt/β-catenin signalling affects differentiation of cells arising from the cerebellar ventricular zone.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/b4f0766821304adc81a311a3fea18090
work_keys_str_mv	AT haydenjselvadurai activationofwntbcateninsignallingaffectsdifferentiationofcellsarisingfromthecerebellarventricularzone AT johnomason activationofwntbcateninsignallingaffectsdifferentiationofcellsarisingfromthecerebellarventricularzone
_version_	1718423875958603776

Activation of Wnt/β-catenin signalling affects differentiation of cells arising from the cerebellar ventricular zone.

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