An S-locus independent pollen factor confers self-compatibility in 'Katy' apricot.

Loss of pollen-S function in Prunus self-compatible cultivars has been mostly associated with deletions or insertions in the S-haplotype-specific F-box (SFB) genes. However, self-compatible pollen-part mutants defective for non-S-locus factors have also been found, for instance, in the apricot (Prun...

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Autores principales:	Elena Zuriaga, Juan V Muñoz-Sanz, Laura Molina, Ana D Gisbert, María L Badenes, Carlos Romero
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b4f0972451e845e1bb0bcaa93cfdcd3a
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spelling	oai:doaj.org-article:b4f0972451e845e1bb0bcaa93cfdcd3a2021-11-18T08:01:34ZAn S-locus independent pollen factor confers self-compatibility in 'Katy' apricot.1932-620310.1371/journal.pone.0053947https://doaj.org/article/b4f0972451e845e1bb0bcaa93cfdcd3a2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23342044/?tool=EBIhttps://doaj.org/toc/1932-6203Loss of pollen-S function in Prunus self-compatible cultivars has been mostly associated with deletions or insertions in the S-haplotype-specific F-box (SFB) genes. However, self-compatible pollen-part mutants defective for non-S-locus factors have also been found, for instance, in the apricot (Prunus armeniaca) cv. 'Canino'. In the present study, we report the genetic and molecular analysis of another self-compatible apricot cv. termed 'Katy'. S-genotype of 'Katy' was determined as S(1)S(2) and S-RNase PCR-typing of selfing and outcrossing populations from 'Katy' showed that pollen gametes bearing either the S(1)- or the S(2)-haplotype were able to overcome self-incompatibility (SI) barriers. Sequence analyses showed no SNP or indel affecting the SFB(1) and SFB(2) alleles from 'Katy' and, moreover, no evidence of pollen-S duplication was found. As a whole, the obtained results are compatible with the hypothesis that the loss-of-function of a S-locus unlinked factor gametophytically expressed in pollen (M'-locus) leads to SI breakdown in 'Katy'. A mapping strategy based on segregation distortion loci mapped the M'-locus within an interval of 9.4 cM at the distal end of chr.3 corresponding to ∼1.29 Mb in the peach (Prunus persica) genome. Interestingly, pollen-part mutations (PPMs) causing self-compatibility (SC) in the apricot cvs. 'Canino' and 'Katy' are located within an overlapping region of ∼273 Kb in chr.3. No evidence is yet available to discern if they affect the same gene or not, but molecular markers seem to indicate that both cultivars are genetically unrelated suggesting that every PPM may have arisen independently. Further research will be necessary to reveal the precise nature of 'Katy' PPM, but fine-mapping already enables SC marker-assisted selection and paves the way for future positional cloning of the underlying gene.Elena ZuriagaJuan V Muñoz-SanzLaura MolinaAna D GisbertMaría L BadenesCarlos RomeroPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e53947 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elena Zuriaga Juan V Muñoz-Sanz Laura Molina Ana D Gisbert María L Badenes Carlos Romero An S-locus independent pollen factor confers self-compatibility in 'Katy' apricot.
description	Loss of pollen-S function in Prunus self-compatible cultivars has been mostly associated with deletions or insertions in the S-haplotype-specific F-box (SFB) genes. However, self-compatible pollen-part mutants defective for non-S-locus factors have also been found, for instance, in the apricot (Prunus armeniaca) cv. 'Canino'. In the present study, we report the genetic and molecular analysis of another self-compatible apricot cv. termed 'Katy'. S-genotype of 'Katy' was determined as S(1)S(2) and S-RNase PCR-typing of selfing and outcrossing populations from 'Katy' showed that pollen gametes bearing either the S(1)- or the S(2)-haplotype were able to overcome self-incompatibility (SI) barriers. Sequence analyses showed no SNP or indel affecting the SFB(1) and SFB(2) alleles from 'Katy' and, moreover, no evidence of pollen-S duplication was found. As a whole, the obtained results are compatible with the hypothesis that the loss-of-function of a S-locus unlinked factor gametophytically expressed in pollen (M'-locus) leads to SI breakdown in 'Katy'. A mapping strategy based on segregation distortion loci mapped the M'-locus within an interval of 9.4 cM at the distal end of chr.3 corresponding to ∼1.29 Mb in the peach (Prunus persica) genome. Interestingly, pollen-part mutations (PPMs) causing self-compatibility (SC) in the apricot cvs. 'Canino' and 'Katy' are located within an overlapping region of ∼273 Kb in chr.3. No evidence is yet available to discern if they affect the same gene or not, but molecular markers seem to indicate that both cultivars are genetically unrelated suggesting that every PPM may have arisen independently. Further research will be necessary to reveal the precise nature of 'Katy' PPM, but fine-mapping already enables SC marker-assisted selection and paves the way for future positional cloning of the underlying gene.
format	article
author	Elena Zuriaga Juan V Muñoz-Sanz Laura Molina Ana D Gisbert María L Badenes Carlos Romero
author_facet	Elena Zuriaga Juan V Muñoz-Sanz Laura Molina Ana D Gisbert María L Badenes Carlos Romero
author_sort	Elena Zuriaga
title	An S-locus independent pollen factor confers self-compatibility in 'Katy' apricot.
title_short	An S-locus independent pollen factor confers self-compatibility in 'Katy' apricot.
title_full	An S-locus independent pollen factor confers self-compatibility in 'Katy' apricot.
title_fullStr	An S-locus independent pollen factor confers self-compatibility in 'Katy' apricot.
title_full_unstemmed	An S-locus independent pollen factor confers self-compatibility in 'Katy' apricot.
title_sort	s-locus independent pollen factor confers self-compatibility in 'katy' apricot.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/b4f0972451e845e1bb0bcaa93cfdcd3a
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An S-locus independent pollen factor confers self-compatibility in 'Katy' apricot.

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