Rapid fabrication of hydrogel micropatterns by projection stereolithography for studying self-organized developmental patterning.

Self-organized patterning of mammalian embryonic stem cells on micropatterned surfaces has previously been established as an in vitro platform for early mammalian developmental studies, complimentary to in vivo studies. Traditional micropatterning methods, such as micro-contact printing (μCP), invol...

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Autores principales:	Ye Zhu, Daniel Sazer, Jordan S Miller, Aryeh Warmflash
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/b4f83b5b33724c88b496438cbccd6ae4
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spelling	oai:doaj.org-article:b4f83b5b33724c88b496438cbccd6ae42021-12-02T20:05:24ZRapid fabrication of hydrogel micropatterns by projection stereolithography for studying self-organized developmental patterning.1932-620310.1371/journal.pone.0245634https://doaj.org/article/b4f83b5b33724c88b496438cbccd6ae42021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0245634https://doaj.org/toc/1932-6203Self-organized patterning of mammalian embryonic stem cells on micropatterned surfaces has previously been established as an in vitro platform for early mammalian developmental studies, complimentary to in vivo studies. Traditional micropatterning methods, such as micro-contact printing (μCP), involve relatively complicated fabrication procedures, which restricts widespread adoption by biologists. Here, we demonstrate a rapid method of micropatterning by printing hydrogel micro-features onto a glass-bottomed culture vessel. The micro-features are printed using a projection stereolithography bioprinter yielding hydrogel structures that geometrically restrict the attachment of cells or proteins. Compared to traditional and physical photomasks, a digitally tunable virtual photomask is used in the projector to generate blue light patterns that enable rapid iteration with minimal cost and effort. We show that a protocol that makes use of this method together with LN521 coating, an extracellular matrix coating, creates a surface suitable for human embryonic stem cell (hESC) attachment and growth with minimal non-specific adhesion. We further demonstrate that self-patterning of hESCs following previously published gastrulation and ectodermal induction protocols achieves results comparable with those obtained with commercially available plates.Ye ZhuDaniel SazerJordan S MillerAryeh WarmflashPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0245634 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ye Zhu Daniel Sazer Jordan S Miller Aryeh Warmflash Rapid fabrication of hydrogel micropatterns by projection stereolithography for studying self-organized developmental patterning.
description	Self-organized patterning of mammalian embryonic stem cells on micropatterned surfaces has previously been established as an in vitro platform for early mammalian developmental studies, complimentary to in vivo studies. Traditional micropatterning methods, such as micro-contact printing (μCP), involve relatively complicated fabrication procedures, which restricts widespread adoption by biologists. Here, we demonstrate a rapid method of micropatterning by printing hydrogel micro-features onto a glass-bottomed culture vessel. The micro-features are printed using a projection stereolithography bioprinter yielding hydrogel structures that geometrically restrict the attachment of cells or proteins. Compared to traditional and physical photomasks, a digitally tunable virtual photomask is used in the projector to generate blue light patterns that enable rapid iteration with minimal cost and effort. We show that a protocol that makes use of this method together with LN521 coating, an extracellular matrix coating, creates a surface suitable for human embryonic stem cell (hESC) attachment and growth with minimal non-specific adhesion. We further demonstrate that self-patterning of hESCs following previously published gastrulation and ectodermal induction protocols achieves results comparable with those obtained with commercially available plates.
format	article
author	Ye Zhu Daniel Sazer Jordan S Miller Aryeh Warmflash
author_facet	Ye Zhu Daniel Sazer Jordan S Miller Aryeh Warmflash
author_sort	Ye Zhu
title	Rapid fabrication of hydrogel micropatterns by projection stereolithography for studying self-organized developmental patterning.
title_short	Rapid fabrication of hydrogel micropatterns by projection stereolithography for studying self-organized developmental patterning.
title_full	Rapid fabrication of hydrogel micropatterns by projection stereolithography for studying self-organized developmental patterning.
title_fullStr	Rapid fabrication of hydrogel micropatterns by projection stereolithography for studying self-organized developmental patterning.
title_full_unstemmed	Rapid fabrication of hydrogel micropatterns by projection stereolithography for studying self-organized developmental patterning.
title_sort	rapid fabrication of hydrogel micropatterns by projection stereolithography for studying self-organized developmental patterning.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/b4f83b5b33724c88b496438cbccd6ae4
work_keys_str_mv	AT yezhu rapidfabricationofhydrogelmicropatternsbyprojectionstereolithographyforstudyingselforganizeddevelopmentalpatterning AT danielsazer rapidfabricationofhydrogelmicropatternsbyprojectionstereolithographyforstudyingselforganizeddevelopmentalpatterning AT jordansmiller rapidfabricationofhydrogelmicropatternsbyprojectionstereolithographyforstudyingselforganizeddevelopmentalpatterning AT aryehwarmflash rapidfabricationofhydrogelmicropatternsbyprojectionstereolithographyforstudyingselforganizeddevelopmentalpatterning
_version_	1718375504478732288

Rapid fabrication of hydrogel micropatterns by projection stereolithography for studying self-organized developmental patterning.

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