Whole cell microalgal-cyanobacterial array biosensor for monitoring Cd, Cr and Zn in aquatic systems

Bioavailable content of metals in aquatic systems has become critical in assessing the toxic effect of metals accumulating in the environment. Considering the need for rapid measurements, an optical microalgal-cyanobacterial array biosensor was developed using two strains of microalgae, Mesotaenium...

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Autores principales:	I. V. N. Rathnayake, Thilini Munagamage, A. Pathirathne, Mallavarapu Megharaj
Formato:	article
Lenguaje:	EN
Publicado:	IWA Publishing 2021
Materias:	array biosensor cyanobacteria fluorescence heavy metals microalgae Environmental technology. Sanitary engineering TD1-1066
Acceso en línea:	https://doaj.org/article/b4fe39a946be40689ce914ad644be455
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spelling	oai:doaj.org-article:b4fe39a946be40689ce914ad644be4552021-11-06T11:24:06ZWhole cell microalgal-cyanobacterial array biosensor for monitoring Cd, Cr and Zn in aquatic systems0273-12231996-973210.2166/wst.2021.339https://doaj.org/article/b4fe39a946be40689ce914ad644be4552021-10-01T00:00:00Zhttp://wst.iwaponline.com/content/84/7/1579https://doaj.org/toc/0273-1223https://doaj.org/toc/1996-9732Bioavailable content of metals in aquatic systems has become critical in assessing the toxic effect of metals accumulating in the environment. Considering the need for rapid measurements, an optical microalgal-cyanobacterial array biosensor was developed using two strains of microalgae, Mesotaenium sp. and a strain of cyanobacteria Synechococcus sp. to detect Cd2+, Cr6+ and Zn2+ in aquatic systems. Microalgal and cyanobacterial cells were immobilized in a 96-well microplate using sol-gel method using silica. Optimum operational conditions for the biosensor array such as exposure time, storage stability, pH, and multiple metal effect were tested. A 10 min exposure time yielded optimum fluorescence values. Metal toxicity increased with decreasing pH, resulting in low relative fluorescence (%) and decreased with increasing pH, resulting in higher relative fluorescence (%). The optimum storage time for biosensor strains were 4 weeks for microalgal cultures and 8 weeks for cyanobacterial culture, at 4 °C storage temperature. The metal mixtures showed less effect on the inhibition of relative fluorescence (%) of microalgal/cyanobacterial cultures, displaying an antagonistic behavior among the metals tested. As a single unit, this photosynthetic array biosensor will be a valuable tool in detecting multi-metals in aquatic systems. HIGHLIGHTS A whole-cell microalgal-cyanobacterial array biosensor was successfully developed for monitoring acute metal toxicity in water systems.; Biosensor organisms respond to metals within 10 minutes' exposure.; Survival potential of the biosensor organisms was higher at high pH.; Antagonistic effect was shown by the biosensor organisms in metal mixtures.; Biosensor was viable throughout 8 weeks of storage at 4 °C.;I. V. N. RathnayakeThilini MunagamageA. PathirathneMallavarapu MegharajIWA Publishingarticlearraybiosensorcyanobacteriafluorescenceheavy metalsmicroalgaeEnvironmental technology. Sanitary engineeringTD1-1066ENWater Science and Technology, Vol 84, Iss 7, Pp 1579-1593 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	array biosensor cyanobacteria fluorescence heavy metals microalgae Environmental technology. Sanitary engineering TD1-1066
spellingShingle	array biosensor cyanobacteria fluorescence heavy metals microalgae Environmental technology. Sanitary engineering TD1-1066 I. V. N. Rathnayake Thilini Munagamage A. Pathirathne Mallavarapu Megharaj Whole cell microalgal-cyanobacterial array biosensor for monitoring Cd, Cr and Zn in aquatic systems
description	Bioavailable content of metals in aquatic systems has become critical in assessing the toxic effect of metals accumulating in the environment. Considering the need for rapid measurements, an optical microalgal-cyanobacterial array biosensor was developed using two strains of microalgae, Mesotaenium sp. and a strain of cyanobacteria Synechococcus sp. to detect Cd2+, Cr6+ and Zn2+ in aquatic systems. Microalgal and cyanobacterial cells were immobilized in a 96-well microplate using sol-gel method using silica. Optimum operational conditions for the biosensor array such as exposure time, storage stability, pH, and multiple metal effect were tested. A 10 min exposure time yielded optimum fluorescence values. Metal toxicity increased with decreasing pH, resulting in low relative fluorescence (%) and decreased with increasing pH, resulting in higher relative fluorescence (%). The optimum storage time for biosensor strains were 4 weeks for microalgal cultures and 8 weeks for cyanobacterial culture, at 4 °C storage temperature. The metal mixtures showed less effect on the inhibition of relative fluorescence (%) of microalgal/cyanobacterial cultures, displaying an antagonistic behavior among the metals tested. As a single unit, this photosynthetic array biosensor will be a valuable tool in detecting multi-metals in aquatic systems. HIGHLIGHTS A whole-cell microalgal-cyanobacterial array biosensor was successfully developed for monitoring acute metal toxicity in water systems.; Biosensor organisms respond to metals within 10 minutes' exposure.; Survival potential of the biosensor organisms was higher at high pH.; Antagonistic effect was shown by the biosensor organisms in metal mixtures.; Biosensor was viable throughout 8 weeks of storage at 4 °C.;
format	article
author	I. V. N. Rathnayake Thilini Munagamage A. Pathirathne Mallavarapu Megharaj
author_facet	I. V. N. Rathnayake Thilini Munagamage A. Pathirathne Mallavarapu Megharaj
author_sort	I. V. N. Rathnayake
title	Whole cell microalgal-cyanobacterial array biosensor for monitoring Cd, Cr and Zn in aquatic systems
title_short	Whole cell microalgal-cyanobacterial array biosensor for monitoring Cd, Cr and Zn in aquatic systems
title_full	Whole cell microalgal-cyanobacterial array biosensor for monitoring Cd, Cr and Zn in aquatic systems
title_fullStr	Whole cell microalgal-cyanobacterial array biosensor for monitoring Cd, Cr and Zn in aquatic systems
title_full_unstemmed	Whole cell microalgal-cyanobacterial array biosensor for monitoring Cd, Cr and Zn in aquatic systems
title_sort	whole cell microalgal-cyanobacterial array biosensor for monitoring cd, cr and zn in aquatic systems
publisher	IWA Publishing
publishDate	2021
url	https://doaj.org/article/b4fe39a946be40689ce914ad644be455
work_keys_str_mv	AT ivnrathnayake wholecellmicroalgalcyanobacterialarraybiosensorformonitoringcdcrandzninaquaticsystems AT thilinimunagamage wholecellmicroalgalcyanobacterialarraybiosensorformonitoringcdcrandzninaquaticsystems AT apathirathne wholecellmicroalgalcyanobacterialarraybiosensorformonitoringcdcrandzninaquaticsystems AT mallavarapumegharaj wholecellmicroalgalcyanobacterialarraybiosensorformonitoringcdcrandzninaquaticsystems
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Whole cell microalgal-cyanobacterial array biosensor for monitoring Cd, Cr and Zn in aquatic systems

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