Identification of male sterile (T, S) of fertility (N) cytoplasm by PCR-based molecular markers to access maintainer lines in onion

Purpose: Modern onion breeding is almost completely based on the production of hybrid seed. This project was performed to evaluate the effectiveness of marker-assisted selection (MAS) in identification of the cytoplasmic types and Ms locus in 123 onion accessions. Research method: Three cytoplasmic...

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Autores principales:	Rahele Dehghani, Zahra Abbasi, Majid Talebi, Emad Shahmansouri, Badrodin Ebrahim Sayed Tabatabaei
Formato:	article
Lenguaje:	EN
Publicado:	University of Birjand 2021
Materias:	allium cepa cytoplasmic male sterility hybrid restoration locus Agriculture S
Acceso en línea:	https://doaj.org/article/b502113fdaff4cd4b44d02960f9759b3
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spelling	oai:doaj.org-article:b502113fdaff4cd4b44d02960f9759b32021-11-06T05:41:58ZIdentification of male sterile (T, S) of fertility (N) cytoplasm by PCR-based molecular markers to access maintainer lines in onion2588-48832588-616910.22077/jhpr.2020.3665.1163https://doaj.org/article/b502113fdaff4cd4b44d02960f9759b32021-09-01T00:00:00Zhttps://jhpr.birjand.ac.ir/article_1590_a4bbbb72ee9b0158f2c3a5784c9c863c.pdfhttps://doaj.org/toc/2588-4883https://doaj.org/toc/2588-6169Purpose: Modern onion breeding is almost completely based on the production of hybrid seed. This project was performed to evaluate the effectiveness of marker-assisted selection (MAS) in identification of the cytoplasmic types and Ms locus in 123 onion accessions. Research method: Three cytoplasmic markers cob, accD and MK were used to identify the sterility (S) from the fertility (N) cytoplasm and four nuclear molecular markers (OPT, PsaO, Jnurf-13 and AcSKP1) were used for genotyping of Ms alleles. Findings: The results showed that the two accD and cob markers were quite similar in the detection of the type of cytoplasm with 100% male sterility for male sterile lines and 100% fertility for maintainer lines. The MK marker was able to distinguish T-type cytoplasm as well. Based on the results, the frequency of fertility (N) was much more than the frequency of sterility (S and T) cytoplasm found to be 90% in Dorche (pop.1), 100% in Dorche (pop.2) and Kashan based on marker cob and accD and with MK marker, was found to be 80%, 90% and 82% in Dorche (pop.1), Dorche (pop.2) and Kashan, respectively. Limitations: In this study, nuclear markers were not successful due to not finding linkage disequilibrium with the Ms locus, suggested more markers to be evaluated. Originality/Value: Molecuar markers were very suitable for the indentification of S or N lines. Cytotype (N/S) determination of plants by usingmolecular markers (cob, accD and MK), could easily reduce the population size required for the production of onion hybrid seeds.Rahele DehghaniZahra AbbasiMajid TalebiEmad ShahmansouriBadrodin Ebrahim Sayed TabatabaeiUniversity of Birjandarticleallium cepacytoplasmic male sterilityhybridrestoration locusAgricultureSENJournal of Horticulture and Postharvest Research, Vol 4, Iss Issue 3 - September 2021, Pp 277-288 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	allium cepa cytoplasmic male sterility hybrid restoration locus Agriculture S
spellingShingle	allium cepa cytoplasmic male sterility hybrid restoration locus Agriculture S Rahele Dehghani Zahra Abbasi Majid Talebi Emad Shahmansouri Badrodin Ebrahim Sayed Tabatabaei Identification of male sterile (T, S) of fertility (N) cytoplasm by PCR-based molecular markers to access maintainer lines in onion
description	Purpose: Modern onion breeding is almost completely based on the production of hybrid seed. This project was performed to evaluate the effectiveness of marker-assisted selection (MAS) in identification of the cytoplasmic types and Ms locus in 123 onion accessions. Research method: Three cytoplasmic markers cob, accD and MK were used to identify the sterility (S) from the fertility (N) cytoplasm and four nuclear molecular markers (OPT, PsaO, Jnurf-13 and AcSKP1) were used for genotyping of Ms alleles. Findings: The results showed that the two accD and cob markers were quite similar in the detection of the type of cytoplasm with 100% male sterility for male sterile lines and 100% fertility for maintainer lines. The MK marker was able to distinguish T-type cytoplasm as well. Based on the results, the frequency of fertility (N) was much more than the frequency of sterility (S and T) cytoplasm found to be 90% in Dorche (pop.1), 100% in Dorche (pop.2) and Kashan based on marker cob and accD and with MK marker, was found to be 80%, 90% and 82% in Dorche (pop.1), Dorche (pop.2) and Kashan, respectively. Limitations: In this study, nuclear markers were not successful due to not finding linkage disequilibrium with the Ms locus, suggested more markers to be evaluated. Originality/Value: Molecuar markers were very suitable for the indentification of S or N lines. Cytotype (N/S) determination of plants by usingmolecular markers (cob, accD and MK), could easily reduce the population size required for the production of onion hybrid seeds.
format	article
author	Rahele Dehghani Zahra Abbasi Majid Talebi Emad Shahmansouri Badrodin Ebrahim Sayed Tabatabaei
author_facet	Rahele Dehghani Zahra Abbasi Majid Talebi Emad Shahmansouri Badrodin Ebrahim Sayed Tabatabaei
author_sort	Rahele Dehghani
title	Identification of male sterile (T, S) of fertility (N) cytoplasm by PCR-based molecular markers to access maintainer lines in onion
title_short	Identification of male sterile (T, S) of fertility (N) cytoplasm by PCR-based molecular markers to access maintainer lines in onion
title_full	Identification of male sterile (T, S) of fertility (N) cytoplasm by PCR-based molecular markers to access maintainer lines in onion
title_fullStr	Identification of male sterile (T, S) of fertility (N) cytoplasm by PCR-based molecular markers to access maintainer lines in onion
title_full_unstemmed	Identification of male sterile (T, S) of fertility (N) cytoplasm by PCR-based molecular markers to access maintainer lines in onion
title_sort	identification of male sterile (t, s) of fertility (n) cytoplasm by pcr-based molecular markers to access maintainer lines in onion
publisher	University of Birjand
publishDate	2021
url	https://doaj.org/article/b502113fdaff4cd4b44d02960f9759b3
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Identification of male sterile (T, S) of fertility (N) cytoplasm by PCR-based molecular markers to access maintainer lines in onion

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