Screening of Parkinson’s Differential MicroRNA Based on GEO Database and Its Clinical Verification

Objective. This study is set out to explore the potential difference of miR in PD through GEO data and provide diagnostic indicators for clinical practice. Methods. In this study, differential miR was screened through the Gene Expression Omnibus (GEO) database, 68 PD patients treated in our hospital...

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Autores principales:	Xuping Jiang, Lili Xiao, Xumei Jiang, Guangsheng Li, Zhijuan Lu
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Medicine R
Acceso en línea:	https://doaj.org/article/b504a22e58a046989373e12ef5cafc3e
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spelling	oai:doaj.org-article:b504a22e58a046989373e12ef5cafc3e2021-11-22T01:11:36ZScreening of Parkinson’s Differential MicroRNA Based on GEO Database and Its Clinical Verification2314-614110.1155/2021/8171236https://doaj.org/article/b504a22e58a046989373e12ef5cafc3e2021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/8171236https://doaj.org/toc/2314-6141Objective. This study is set out to explore the potential difference of miR in PD through GEO data and provide diagnostic indicators for clinical practice. Methods. In this study, differential miR was screened through the Gene Expression Omnibus (GEO) database, 68 PD patients treated in our hospital from May 2017 to March 2018 were collected as the research group (RG), and 50 normal subjects who underwent physical examination in our hospital during the same period were collected as the control group (CG). Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression and diagnostic value of miR-374a-5p in serum of patients. The potential target genes of miR-374a-5p were predicted, and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and Gene Ontology Consortium (GO) were carried out. Results. GEO2R analysis revealed that 193 miRs are expressed differentially, of which 78 were highly expressed and 115 were poorly expressed. The miR-374a-5p expression in the serum of the RG was reduced markedly and had a diagnostic value. Targetscan and miRDB online websites were used to predict their target genes, with 415 common target genes. miR-374a-5p may participate in 27 functional pathways and 8 signal pathways. Conclusion. miR-335-5p has low expression in PD and is expected to be a potential diagnostic indicator.Xuping JiangLili XiaoXumei JiangGuangsheng LiZhijuan LuHindawi LimitedarticleMedicineRENBioMed Research International, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R
spellingShingle	Medicine R Xuping Jiang Lili Xiao Xumei Jiang Guangsheng Li Zhijuan Lu Screening of Parkinson’s Differential MicroRNA Based on GEO Database and Its Clinical Verification
description	Objective. This study is set out to explore the potential difference of miR in PD through GEO data and provide diagnostic indicators for clinical practice. Methods. In this study, differential miR was screened through the Gene Expression Omnibus (GEO) database, 68 PD patients treated in our hospital from May 2017 to March 2018 were collected as the research group (RG), and 50 normal subjects who underwent physical examination in our hospital during the same period were collected as the control group (CG). Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression and diagnostic value of miR-374a-5p in serum of patients. The potential target genes of miR-374a-5p were predicted, and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and Gene Ontology Consortium (GO) were carried out. Results. GEO2R analysis revealed that 193 miRs are expressed differentially, of which 78 were highly expressed and 115 were poorly expressed. The miR-374a-5p expression in the serum of the RG was reduced markedly and had a diagnostic value. Targetscan and miRDB online websites were used to predict their target genes, with 415 common target genes. miR-374a-5p may participate in 27 functional pathways and 8 signal pathways. Conclusion. miR-335-5p has low expression in PD and is expected to be a potential diagnostic indicator.
format	article
author	Xuping Jiang Lili Xiao Xumei Jiang Guangsheng Li Zhijuan Lu
author_facet	Xuping Jiang Lili Xiao Xumei Jiang Guangsheng Li Zhijuan Lu
author_sort	Xuping Jiang
title	Screening of Parkinson’s Differential MicroRNA Based on GEO Database and Its Clinical Verification
title_short	Screening of Parkinson’s Differential MicroRNA Based on GEO Database and Its Clinical Verification
title_full	Screening of Parkinson’s Differential MicroRNA Based on GEO Database and Its Clinical Verification
title_fullStr	Screening of Parkinson’s Differential MicroRNA Based on GEO Database and Its Clinical Verification
title_full_unstemmed	Screening of Parkinson’s Differential MicroRNA Based on GEO Database and Its Clinical Verification
title_sort	screening of parkinson’s differential microrna based on geo database and its clinical verification
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/b504a22e58a046989373e12ef5cafc3e
work_keys_str_mv	AT xupingjiang screeningofparkinsonsdifferentialmicrornabasedongeodatabaseanditsclinicalverification AT lilixiao screeningofparkinsonsdifferentialmicrornabasedongeodatabaseanditsclinicalverification AT xumeijiang screeningofparkinsonsdifferentialmicrornabasedongeodatabaseanditsclinicalverification AT guangshengli screeningofparkinsonsdifferentialmicrornabasedongeodatabaseanditsclinicalverification AT zhijuanlu screeningofparkinsonsdifferentialmicrornabasedongeodatabaseanditsclinicalverification
_version_	1718418301882728448

Screening of Parkinson’s Differential MicroRNA Based on GEO Database and Its Clinical Verification

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