Dopamine-functionalized InP/ZnS quantum dots as fluorescence probes for the detection of adenosine in microfluidic chip
Seshadri Reddy Ankireddy, Jongsung Kim Department of Chemical and Biological Engineering, Gachon University, Seongnam, Gyeonggi-Do, South Korea Abstract: Microbeads are frequently used as solid supports for biomolecules such as proteins and nucleic acids in heterogeneous microfluidic assays. Chip-...
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| Formato: | article |
| Lenguaje: | EN |
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Dove Medical Press
2015
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| Acceso en línea: | https://doaj.org/article/b50f38e0bf22415c84657a5484e45d40 |
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| Sumario: | Seshadri Reddy Ankireddy, Jongsung Kim Department of Chemical and Biological Engineering, Gachon University, Seongnam, Gyeonggi-Do, South Korea Abstract: Microbeads are frequently used as solid supports for biomolecules such as proteins and nucleic acids in heterogeneous microfluidic assays. Chip-based, quantum dot (QD)-bead-biomolecule probes have been used for the detection of various types of DNA. In this study, we developed dopamine (DA)-functionalized InP/ZnS QDs (QDs-DA) as fluorescence probes for the detection of adenosine in microfluidic chips. The photoluminescence (PL) intensity of the QDs-DA is quenched by Zn2+ because of the strong coordination interactions. In the presence of adenosine, Zn2+ cations preferentially bind to adenosine, and the PL intensity of the QDs-DA is recovered. A polydimethylsiloxane-based microfluidic chip was fabricated, and adenosine detection was confirmed using QDs-DA probes. Keywords: quantum dots, photoluminescence, microfluidic chip, adenosine, mercaptopropionic acid, mercaptoundecanoic acid, PDMS |
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