Dopamine-functionalized InP/ZnS quantum dots as fluorescence probes for the detection of adenosine in microfluidic chip

Seshadri Reddy Ankireddy, Jongsung Kim Department of Chemical and Biological Engineering, Gachon University, Seongnam, Gyeonggi-Do, South Korea Abstract: Microbeads are frequently used as solid supports for biomolecules such as proteins and nucleic acids in heterogeneous microfluidic assays. Chip-...

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Autores principales:	Ankireddy SR, Kim J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/b50f38e0bf22415c84657a5484e45d40
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spelling	oai:doaj.org-article:b50f38e0bf22415c84657a5484e45d402021-12-02T08:07:34ZDopamine-functionalized InP/ZnS quantum dots as fluorescence probes for the detection of adenosine in microfluidic chip1178-2013https://doaj.org/article/b50f38e0bf22415c84657a5484e45d402015-08-01T00:00:00Zhttp://www.dovepress.com/dopamine-functionalized-inpzns-quantum-dots-as-fluorescence-probes-for-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Seshadri Reddy Ankireddy, Jongsung Kim Department of Chemical and Biological Engineering, Gachon University, Seongnam, Gyeonggi-Do, South Korea Abstract: Microbeads are frequently used as solid supports for biomolecules such as proteins and nucleic acids in heterogeneous microfluidic assays. Chip-based, quantum dot (QD)-bead-biomolecule probes have been used for the detection of various types of DNA. In this study, we developed dopamine (DA)-functionalized InP/ZnS QDs (QDs-DA) as fluorescence probes for the detection of adenosine in microfluidic chips. The photoluminescence (PL) intensity of the QDs-DA is quenched by Zn2+ because of the strong coordination interactions. In the presence of adenosine, Zn2+ cations preferentially bind to adenosine, and the PL intensity of the QDs-DA is recovered. A polydimethylsiloxane-based microfluidic chip was fabricated, and adenosine detection was confirmed using QDs-DA probes. Keywords: quantum dots, photoluminescence, microfluidic chip, adenosine, mercaptopropionic acid, mercaptoundecanoic acid, PDMSAnkireddy SRKim JDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss Special Issue on diverse applications in Nano-Theranostics, Pp 121-128 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Ankireddy SR Kim J Dopamine-functionalized InP/ZnS quantum dots as fluorescence probes for the detection of adenosine in microfluidic chip
description	Seshadri Reddy Ankireddy, Jongsung Kim Department of Chemical and Biological Engineering, Gachon University, Seongnam, Gyeonggi-Do, South Korea Abstract: Microbeads are frequently used as solid supports for biomolecules such as proteins and nucleic acids in heterogeneous microfluidic assays. Chip-based, quantum dot (QD)-bead-biomolecule probes have been used for the detection of various types of DNA. In this study, we developed dopamine (DA)-functionalized InP/ZnS QDs (QDs-DA) as fluorescence probes for the detection of adenosine in microfluidic chips. The photoluminescence (PL) intensity of the QDs-DA is quenched by Zn2+ because of the strong coordination interactions. In the presence of adenosine, Zn2+ cations preferentially bind to adenosine, and the PL intensity of the QDs-DA is recovered. A polydimethylsiloxane-based microfluidic chip was fabricated, and adenosine detection was confirmed using QDs-DA probes. Keywords: quantum dots, photoluminescence, microfluidic chip, adenosine, mercaptopropionic acid, mercaptoundecanoic acid, PDMS
format	article
author	Ankireddy SR Kim J
author_facet	Ankireddy SR Kim J
author_sort	Ankireddy SR
title	Dopamine-functionalized InP/ZnS quantum dots as fluorescence probes for the detection of adenosine in microfluidic chip
title_short	Dopamine-functionalized InP/ZnS quantum dots as fluorescence probes for the detection of adenosine in microfluidic chip
title_full	Dopamine-functionalized InP/ZnS quantum dots as fluorescence probes for the detection of adenosine in microfluidic chip
title_fullStr	Dopamine-functionalized InP/ZnS quantum dots as fluorescence probes for the detection of adenosine in microfluidic chip
title_full_unstemmed	Dopamine-functionalized InP/ZnS quantum dots as fluorescence probes for the detection of adenosine in microfluidic chip
title_sort	dopamine-functionalized inp/zns quantum dots as fluorescence probes for the detection of adenosine in microfluidic chip
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/b50f38e0bf22415c84657a5484e45d40
work_keys_str_mv	AT ankireddysr dopaminefunctionalizedinpznsquantumdotsasfluorescenceprobesforthedetectionofadenosineinmicrofluidicchip AT kimj dopaminefunctionalizedinpznsquantumdotsasfluorescenceprobesforthedetectionofadenosineinmicrofluidicchip
_version_	1718398681407815680

Dopamine-functionalized InP/ZnS quantum dots as fluorescence probes for the detection of adenosine in microfluidic chip

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